Restriction map for
pFN21AE1431
(Linear) (Six-base) MAPSORT of: KIEE1431.seq Check: 9348 from: 1 to: 2292
KIEE1431.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 439 2292
Size: 439 1853
AatII G_ACGT'C
Cuts at: 0 1115 2292
Size: 1115 1177
AccI GT'mk_AC
Cuts at: 0 1934 2292
Size: 1934 358
Acc65I G'GTAC_C
Cuts at: 0 1459 2199 2292
Size: 1459 740 93
Fragments arranged by size:
1459 740 93
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 779 995 1142 2275 2292
Size: 274 505 216 147 1133 17
Fragments arranged by size:
1133 505 274 216 147 17
AflIII A'CryG_T
Cuts at: 0 179 217 610 1350 2292
Size: 179 38 393 740 942
Fragments arranged by size:
942 740 393 179 38
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2219 2292
Size: 2219 73
AlwNI CAG_nnn'CTG
Cuts at: 0 171 1422 2292
Size: 171 1251 870
Fragments arranged by size:
1251 870 171
ApoI r'AATT_y
Cuts at: 0 956 1703 1711 2292
Size: 956 747 8 581
Fragments arranged by size:
956 747 581 8
AseI AT'TA_AT
Cuts at: 0 1682 2292
Size: 1682 610
AvaI C'yCGr_G
Cuts at: 0 501 1811 2213 2292
Size: 501 1310 402 79
Fragments arranged by size:
1310 501 402 79
BamHI G'GATC_C
Cuts at: 0 103 2292
Size: 103 2189
BanI G'GyrC_C
Cuts at: 0 1459 2199 2292
Size: 1459 740 93
Fragments arranged by size:
1459 740 93
BanII G_rGCy'C
Cuts at: 0 418 2292
Size: 418 1874
BbsI GAAGACnn'nnnn_
Cuts at: 0 262 557 698 1040 2292
Size: 262 295 141 342 1252
Fragments arranged by size:
1252 342 295 262 141
BbvCI CC'TCA_GC
Cuts at: 0 740 2292
Size: 740 1552
BclI T'GATC_A
Cuts at: 0 1736 2292
Size: 1736 556
BglI GCCn_nnn'nGGC
Cuts at: 0 1078 2292
Size: 1078 1214
BglII A'GATC_T
Cuts at: 0 229 377 1493 2292
Size: 229 148 1116 799
Fragments arranged by size:
1116 799 229 148
Bme1580I G_kGCm'C
Cuts at: 0 752 2292
Size: 752 1540
BmrI ACTGGGnnnn_n'
Cuts at: 0 1008 2292
Size: 1008 1284
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1844 1994 2292
Size: 1844 150 298
Fragments arranged by size:
1844 298 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 136 193 264 1060 1663 2069 2292
Size: 80 56 57 71 796 603 406 223
Fragments arranged by size:
796 603 406 223 80 71 57 56
Bpu10I CC'TnA_GC
Cuts at: 0 740 2292
Size: 740 1552
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 665 1729 2292
Size: 245 420 1064 563
Fragments arranged by size:
1064 563 420 245
BsaI GGTCTCn'nnnn_
Cuts at: 0 356 712 2292
Size: 356 356 1580
Fragments arranged by size:
1580 356 356
BsaAI yAC'GTr
Cuts at: 0 180 220 2292
Size: 180 40 2072
Fragments arranged by size:
2072 180 40
BsaHI Gr'CG_yC
Cuts at: 0 1112 2292
Size: 1112 1180
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 60 90 166 196 2292
Size: 60 30 76 30 2096
Fragments arranged by size:
2096 76 60 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1918 1921 2066 2292
Size: 1918 3 145 226
Fragments arranged by size:
1918 226 145 3
BseYI C'CCAG_C
Cuts at: 0 599 2153 2292
Size: 599 1554 139
Fragments arranged by size:
1554 599 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 312 1779 1856 2292
Size: 232 80 1467 77 436
Fragments arranged by size:
1467 436 232 80 77
BsiHKAI G_wGCw'C
Cuts at: 0 250 2292
Size: 250 2042
Bsp1286I G_dGCh'C
Cuts at: 0 250 418 752 2292
Size: 250 168 334 1540
Fragments arranged by size:
1540 334 250 168
BspHI T'CATG_A
Cuts at: 0 1157 2292
Size: 1157 1135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 232 439 2187 2211 2292
Size: 232 207 1748 24 81
Fragments arranged by size:
1748 232 207 81 24
BsrBI CCG'CTC
Cuts at: 0 484 635 2292
Size: 484 151 1657
Fragments arranged by size:
1657 484 151
BsrFI r'CCGG_y
Cuts at: 0 446 2292
Size: 446 1846
BsrGI T'GTAC_A
Cuts at: 0 1085 2292
Size: 1085 1207
BssSI C'ACGA_G
Cuts at: 0 1522 2292
Size: 1522 770
BstEII G'GTnAC_C
Cuts at: 0 2025 2292
Size: 2025 267
BstXI CCAn_nnnn'nTGG
Cuts at: 0 442 2292
Size: 442 1850
BstYI r'GATC_y
Cuts at: 0 103 229 377 1211 1493 2292
Size: 103 126 148 834 282 799
Fragments arranged by size:
834 799 282 148 126 103
BtgI C'CryG_G
Cuts at: 0 79 1480 2292
Size: 79 1401 812
Fragments arranged by size:
1401 812 79
BtsI GCAGTG_nn'
Cuts at: 0 387 567 1088 2292
Size: 387 180 521 1204
Fragments arranged by size:
1204 521 387 180
ClaI AT'CG_AT
Cuts at: 0 1406 2292
Size: 1406 886
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 509 544 2292
Size: 509 35 1748
Fragments arranged by size:
1748 509 35
EaeI y'GGCC_r
Cuts at: 0 444 689 1102 1764 2292
Size: 444 245 413 662 528
Fragments arranged by size:
662 528 444 413 245
EarI CTCTTCn'nnn_
Cuts at: 0 1853 2292
Size: 1853 439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 136 193 264 274 779 995 1060
Size: 80 56 57 71 10 505 216 65
Cuts at: 1060 1142 1663 2069 2275 2292
Size: 82 521 406 206 17
Fragments arranged by size:
521 505 406 216 206 82 80 71
65 57 56 17 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1121 2292
Size: 1121 1171
EcoO109I rG'GnC_Cy
Cuts at: 0 239 1071 1188 1638 2292
Size: 239 832 117 450 654
Fragments arranged by size:
832 654 450 239 117
EcoRI G'AATT_C
Cuts at: 0 1703 2292
Size: 1703 589
EcoRV GAT'ATC
Cuts at: 0 1881 2292
Size: 1881 411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 60 92 364 396 2292
Size: 60 32 272 32 1896
Fragments arranged by size:
1896 272 60 32 32
HincII GTy'rAC
Cuts at: 0 882 2292
Size: 882 1410
HindIII A'AGCT_T
Cuts at: 0 695 2292
Size: 695 1597
KpnI G_GTAC'C
Cuts at: 0 1463 2203 2292
Size: 1463 740 89
Fragments arranged by size:
1463 740 89
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 521 1855 2292
Size: 171 350 1334 437
Fragments arranged by size:
1334 437 350 171
MscI TGG'CCA
Cuts at: 0 1104 1766 2292
Size: 1104 662 526
Fragments arranged by size:
1104 662 526
MslI CAynn'nnrTG
Cuts at: 0 392 1156 2292
Size: 392 764 1136
Fragments arranged by size:
1136 764 392
MspA1I CmG'CkG
Cuts at: 0 603 1964 2153 2292
Size: 603 1361 189 139
Fragments arranged by size:
1361 603 189 139
NcoI C'CATG_G
Cuts at: 0 79 1480 2292
Size: 79 1401 812
Fragments arranged by size:
1401 812 79
NdeI CA'TA_TG
Cuts at: 0 939 2292
Size: 939 1353
NspI r_CATG'y
Cuts at: 0 1354 2292
Size: 1354 938
PasI CC'CwG_GG
Cuts at: 0 99 757 2292
Size: 99 658 1535
Fragments arranged by size:
1535 658 99
PciI A'CATG_T
Cuts at: 0 1350 2292
Size: 1350 942
PflMI CCAn_nnn'nTGG
Cuts at: 0 80 171 925 959 2292
Size: 80 91 754 34 1333
Fragments arranged by size:
1333 754 91 80 34
PfoI T'CCnGG_A
Cuts at: 0 241 785 1214 2292
Size: 241 544 429 1078
Fragments arranged by size:
1078 544 429 241
PpuMI rG'GwC_Cy
Cuts at: 0 239 1188 1638 2292
Size: 239 949 450 654
Fragments arranged by size:
949 654 450 239
PspXI vC'TCGA_Gb
Cuts at: 0 1811 2292
Size: 1811 481
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 322 354 2292
Size: 322 32 1938
Fragments arranged by size:
1938 322 32
PstI C_TGCA'G
Cuts at: 0 229 574 2292
Size: 229 345 1718
Fragments arranged by size:
1718 345 229
PvuII CAG'CTG
Cuts at: 0 1964 2153 2292
Size: 1964 189 139
Fragments arranged by size:
1964 189 139
SanDI GG'GwC_CC
Cuts at: 0 1188 2292
Size: 1188 1104
SapI GCTCTTCn'nnn_
Cuts at: 0 1853 2292
Size: 1853 439
ScaI AGT'ACT
Cuts at: 0 1555 2292
Size: 1555 737
SexAI A'CCwGG_T
Cuts at: 0 815 2292
Size: 815 1477
SfcI C'TryA_G
Cuts at: 0 225 570 2292
Size: 225 345 1722
Fragments arranged by size:
1722 345 225
SmaI CCC'GGG
Cuts at: 0 503 2292
Size: 503 1789
SmlI C'TyrA_G
Cuts at: 0 260 680 1744 1811 2292
Size: 260 420 1064 67 481
Fragments arranged by size:
1064 481 420 260 67
StyI C'CwwG_G
Cuts at: 0 79 1252 1480 2292
Size: 79 1173 228 812
Fragments arranged by size:
1173 812 228 79
TatI w'GTAC_w
Cuts at: 0 1085 1553 2044 2292
Size: 1085 468 491 248
Fragments arranged by size:
1085 491 468 248
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 166 198 2292
Size: 166 32 2094
Fragments arranged by size:
2094 166 32
Tth111I GACn'n_nGTC
Cuts at: 0 556 1000 2002 2292
Size: 556 444 1002 290
Fragments arranged by size:
1002 556 444 290
XhoI C'TCGA_G
Cuts at: 0 1811 2292
Size: 1811 481
XmaI C'CCGG_G
Cuts at: 0 501 2292
Size: 501 1791
ZraI GAC'GTC
Cuts at: 0 1113 2292
Size: 1113 1179
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AlfI AlwNI
ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI
BclI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI
BstEII BstXI BstYI BtgI BtsI ClaI CspCI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I HincII
HindIII KpnI MmeI MscI MslI MspA1I NcoI NdeI
NspI PasI PciI PflMI PfoI PpuMI PspXI PsrI
PstI PvuII SanDI SapI ScaI SexAI SfcI SmaI
SmlI StyI TatI TstI Tth111I XhoI XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI AleI AloI ApaI
ApaLI AscI AsiSI AvrII BaeI BbeI BcgI BciVI
BfrBI BlpI BmgBI BmtI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BsrDI BssHII BstAPI BstBI BstZ17I
Bsu36I BtgZI DraI DraIII DrdI EagI EciI EcoICRI
FalI FseI FspI FspAI HaeII HpaI KasI MfeI
MluI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PshAI PsiI PspOMI PvuI
RsrII SacI SacII SalI SbfI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII XbaI XcmI XmnI