Restriction map for
pFN21AE1430
(Linear) (Six-base) MAPSORT of: KIEE1430.seq Check: 2636 from: 1 to: 846
KIEE1430.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 93 846
Size: 93 753
ApaLI G'TGCA_C
Cuts at: 0 114 846
Size: 114 732
ApoI r'AATT_y
Cuts at: 0 284 846
Size: 284 562
BanI G'GyrC_C
Cuts at: 0 102 846
Size: 102 744
BanII G_rGCy'C
Cuts at: 0 40 776 846
Size: 40 736 70
Fragments arranged by size:
736 70 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 593 824 846
Size: 593 231 22
Fragments arranged by size:
593 231 22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 342 376 846
Size: 342 34 470
Fragments arranged by size:
470 342 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 654 846
Size: 654 192
BglI GCCn_nnn'nGGC
Cuts at: 0 84 129 846
Size: 84 45 717
Fragments arranged by size:
717 84 45
BlpI GC'TnA_GC
Cuts at: 0 136 775 846
Size: 136 639 71
Fragments arranged by size:
639 136 71
Bme1580I G_kGCm'C
Cuts at: 0 26 118 846
Size: 26 92 728
Fragments arranged by size:
728 92 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 705 846
Size: 705 141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 462 846
Size: 462 384
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 808 846
Size: 338 470 38
Fragments arranged by size:
470 338 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 846
Size: 808 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 477 507 846
Size: 477 30 339
Fragments arranged by size:
477 339 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 451 846
Size: 30 421 395
Fragments arranged by size:
421 395 30
BseYI C'CCAG_C
Cuts at: 0 33 846
Size: 33 813
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 846
Size: 205 641
BsiEI CG_ry'CG
Cuts at: 0 166 735 846
Size: 166 569 111
Fragments arranged by size:
569 166 111
BsiHKAI G_wGCw'C
Cuts at: 0 118 776 794 846
Size: 118 658 18 52
Fragments arranged by size:
658 118 52 18
Bsp1286I G_dGCh'C
Cuts at: 0 26 40 118 776 794 846
Size: 26 14 78 658 18 52
Fragments arranged by size:
658 78 52 26 18 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 139 846
Size: 139 707
BsrBI CCG'CTC
Cuts at: 0 159 846
Size: 159 687
BsrFI r'CCGG_y
Cuts at: 0 49 99 846
Size: 49 50 747
Fragments arranged by size:
747 50 49
BssHII G'CGCG_C
Cuts at: 0 144 846
Size: 144 702
BstAPI GCAn_nnn'nTGC
Cuts at: 0 93 846
Size: 93 753
BstXI CCAn_nnnn'nTGG
Cuts at: 0 504 846
Size: 504 342
EaeI y'GGCC_r
Cuts at: 0 163 846
Size: 163 683
EagI C'GGCC_G
Cuts at: 0 163 846
Size: 163 683
EcoICRI GAG'CTC
Cuts at: 0 774 846
Size: 774 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 808 846
Size: 338 470 38
Fragments arranged by size:
470 338 38
EcoO109I rG'GnC_Cy
Cuts at: 0 540 846
Size: 540 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 477 509 595 627 846
Size: 477 32 86 32 219
Fragments arranged by size:
477 219 86 32 32
HindIII A'AGCT_T
Cuts at: 0 239 846
Size: 239 607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 558 846
Size: 530 28 288
Fragments arranged by size:
530 288 28
NaeI GCC'GGC
Cuts at: 0 51 101 846
Size: 51 50 745
Fragments arranged by size:
745 51 50
NgoMIV G'CCGG_C
Cuts at: 0 49 99 846
Size: 49 50 747
Fragments arranged by size:
747 50 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 717 846
Size: 717 129
PsiI TTA'TAA
Cuts at: 0 517 846
Size: 517 329
PstI C_TGCA'G
Cuts at: 0 98 846
Size: 98 748
SacI G_AGCT'C
Cuts at: 0 776 846
Size: 776 70
SfcI C'TryA_G
Cuts at: 0 94 846
Size: 94 752
SmlI C'TyrA_G
Cuts at: 0 823 846
Size: 823 23
StyI C'CwwG_G
Cuts at: 0 689 846
Size: 689 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 749 846
Size: 749 97
TatI w'GTAC_w
Cuts at: 0 800 846
Size: 800 46
Enzymes that do cut:
AlwNI ApaLI ApoI BanI BanII BbsI BcgI BciVI
BglI BlpI Bme1580I BmrI BplI BpmI BpuEI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BstAPI BstXI EaeI EagI EcoICRI Eco57MI
EcoO109I Hin4I HindIII MmeI NaeI NgoMIV PflMI PsiI
PstI SacI SfcI SmlI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI
BclI BfrBI BglII BmgBI BmtI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssSI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EarI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI