Restriction map for
pFN21AE1428
(Linear) (Six-base) MAPSORT of: KIEE1428.seq Check: 9122 from: 1 to: 930
KIEE1428.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 138 930
Size: 138 792
AflIII A'CryG_T
Cuts at: 0 332 930
Size: 332 598
AhdI GACnn_n'nnGTC
Cuts at: 0 57 930
Size: 57 873
AleI CACnn'nnGTG
Cuts at: 0 572 930
Size: 572 358
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 911 930
Size: 911 19
ApoI r'AATT_y
Cuts at: 0 517 930
Size: 517 413
AvaI C'yCGr_G
Cuts at: 0 160 930
Size: 160 770
BamHI G'GATC_C
Cuts at: 0 531 930
Size: 531 399
BanI G'GyrC_C
Cuts at: 0 452 930
Size: 452 478
BanII G_rGCy'C
Cuts at: 0 167 930
Size: 167 763
BbvCI CC'TCA_GC
Cuts at: 0 358 448 930
Size: 358 90 482
Fragments arranged by size:
482 358 90
BciVI GTATCCnnnnn_n'
Cuts at: 0 763 930
Size: 763 167
BglI GCCn_nnn'nGGC
Cuts at: 0 344 930
Size: 344 586
BmrI ACTGGGnnnn_n'
Cuts at: 0 54 162 698 930
Size: 54 108 536 232
Fragments arranged by size:
536 232 108 54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 255 817 930
Size: 255 562 113
Fragments arranged by size:
562 255 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 388 439 930
Size: 169 219 51 491
Fragments arranged by size:
491 219 169 51
Bpu10I CC'TnA_GC
Cuts at: 0 358 448 930
Size: 358 90 482
Fragments arranged by size:
482 358 90
BsaI GGTCTCn'nnnn_
Cuts at: 0 790 930
Size: 790 140
BsaAI yAC'GTr
Cuts at: 0 335 930
Size: 335 595
BsaBI GATnn'nnATC
Cuts at: 0 667 930
Size: 667 263
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 319 349 930
Size: 319 30 581
Fragments arranged by size:
581 319 30
BseYI C'CCAG_C
Cuts at: 0 65 121 289 649 930
Size: 65 56 168 360 281
Fragments arranged by size:
360 281 168 65 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 570 801 930
Size: 570 231 129
Fragments arranged by size:
570 231 129
BsiHKAI G_wGCw'C
Cuts at: 0 185 404 930
Size: 185 219 526
Fragments arranged by size:
526 219 185
Bsp1286I G_dGCh'C
Cuts at: 0 167 185 404 930
Size: 167 18 219 526
Fragments arranged by size:
526 219 167 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 138 930
Size: 138 792
BstYI r'GATC_y
Cuts at: 0 531 930
Size: 531 399
BtsI GCAGTG_nn'
Cuts at: 0 582 930
Size: 582 348
EaeI y'GGCC_r
Cuts at: 0 345 704 930
Size: 345 359 226
Fragments arranged by size:
359 345 226
EarI CTCTTCn'nnn_
Cuts at: 0 660 899 930
Size: 660 239 31
Fragments arranged by size:
660 239 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 388 439 930
Size: 169 219 51 491
Fragments arranged by size:
491 219 169 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 425 457 744 776 930
Size: 43 32 350 32 287 32 154
Fragments arranged by size:
350 287 154 43 32 32 32
MscI TGG'CCA
Cuts at: 0 347 930
Size: 347 583
MslI CAynn'nnrTG
Cuts at: 0 572 893 930
Size: 572 321 37
Fragments arranged by size:
572 321 37
MspA1I CmG'CkG
Cuts at: 0 97 323 461 930
Size: 97 226 138 469
Fragments arranged by size:
469 226 138 97
NdeI CA'TA_TG
Cuts at: 0 80 930
Size: 80 850
NspI r_CATG'y
Cuts at: 0 212 930
Size: 212 718
PflMI CCAn_nnn'nTGG
Cuts at: 0 248 930
Size: 248 682
PfoI T'CCnGG_A
Cuts at: 0 185 425 930
Size: 185 240 505
Fragments arranged by size:
505 240 185
PmlI CAC'GTG
Cuts at: 0 335 930
Size: 335 595
PspXI vC'TCGA_Gb
Cuts at: 0 160 930
Size: 160 770
PstI C_TGCA'G
Cuts at: 0 135 742 930
Size: 135 607 188
Fragments arranged by size:
607 188 135
PvuII CAG'CTG
Cuts at: 0 97 461 930
Size: 97 364 469
Fragments arranged by size:
469 364 97
SapI GCTCTTCn'nnn_
Cuts at: 0 660 899 930
Size: 660 239 31
Fragments arranged by size:
660 239 31
SfcI C'TryA_G
Cuts at: 0 131 294 698 719 738 930
Size: 131 163 404 21 19 192
Fragments arranged by size:
404 192 163 131 21 19
SmlI C'TyrA_G
Cuts at: 0 160 930
Size: 160 770
StyI C'CwwG_G
Cuts at: 0 70 930
Size: 70 860
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 292 930
Size: 292 638
TatI w'GTAC_w
Cuts at: 0 329 611 930
Size: 329 282 319
Fragments arranged by size:
329 319 282
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 319 351 930
Size: 319 32 579
Fragments arranged by size:
579 319 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 568 930
Size: 568 362
XhoI C'TCGA_G
Cuts at: 0 160 930
Size: 160 770
Enzymes that do cut:
AarI AflIII AhdI AleI AlfI ApoI AvaI BamHI
BanI BanII BbvCI BciVI BglI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaBI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BspMI BstYI BtsI EaeI EarI Eco57MI Hin4I
MscI MslI MspA1I NdeI NspI PflMI PfoI PmlI
PspXI PstI PvuII SapI SfcI SmlI StyI TaqII
TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BcgI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmtI BpuEI BsaHI BsaWI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XmaI XmnI ZraI