Restriction map for
pFN21AE1426
(Linear) (Six-base) MAPSORT of: KIEE1426.seq Check: 2182 from: 1 to: 1257
KIEE1426.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AccI GT'mk_AC
Cuts at: 0 476 1257
Size: 476 781
AflIII A'CryG_T
Cuts at: 0 740 1257
Size: 740 517
ApoI r'AATT_y
Cuts at: 0 322 997 1257
Size: 322 675 260
Fragments arranged by size:
675 322 260
AvaI C'yCGr_G
Cuts at: 0 57 1257
Size: 57 1200
BanI G'GyrC_C
Cuts at: 0 72 1257
Size: 72 1185
BanII G_rGCy'C
Cuts at: 0 128 1257
Size: 128 1129
BbeI G_GCGC'C
Cuts at: 0 76 1257
Size: 76 1181
BbvCI CC'TCA_GC
Cuts at: 0 541 1257
Size: 541 716
BfrBI ATG'CAT
Cuts at: 0 392 632 1257
Size: 392 240 625
Fragments arranged by size:
625 392 240
BglII A'GATC_T
Cuts at: 0 162 350 1257
Size: 162 188 907
Fragments arranged by size:
907 188 162
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 401 1257
Size: 401 856
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 824 1257
Size: 187 637 433
Fragments arranged by size:
637 433 187
Bpu10I CC'TnA_GC
Cuts at: 0 253 541 773 1257
Size: 253 288 232 484
Fragments arranged by size:
484 288 253 232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 563 1257
Size: 285 278 694
Fragments arranged by size:
694 285 278
BsaI GGTCTCn'nnnn_
Cuts at: 0 859 1257
Size: 859 398
BsaHI Gr'CG_yC
Cuts at: 0 73 1257
Size: 73 1184
BsaWI w'CCGG_w
Cuts at: 0 100 1257
Size: 100 1157
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 160 190 1257
Size: 160 30 1067
Fragments arranged by size:
1067 160 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 1257
Size: 184 1073
BseYI C'CCAG_C
Cuts at: 0 9 973 1257
Size: 9 964 284
Fragments arranged by size:
964 284 9
Bsp1286I G_dGCh'C
Cuts at: 0 128 1257
Size: 128 1129
BspEI T'CCGG_A
Cuts at: 0 100 1257
Size: 100 1157
BspHI T'CATG_A
Cuts at: 0 206 1257
Size: 206 1051
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 733 1045 1257
Size: 733 312 212
Fragments arranged by size:
733 312 212
BsrBI CCG'CTC
Cuts at: 0 889 1257
Size: 889 368
BsrGI T'GTAC_A
Cuts at: 0 239 1084 1257
Size: 239 845 173
Fragments arranged by size:
845 239 173
BssSI C'ACGA_G
Cuts at: 0 531 1257
Size: 531 726
BstXI CCAn_nnnn'nTGG
Cuts at: 0 230 1257
Size: 230 1027
BstYI r'GATC_y
Cuts at: 0 162 350 1257
Size: 162 188 907
Fragments arranged by size:
907 188 162
BtgI C'CryG_G
Cuts at: 0 143 397 1257
Size: 143 254 860
Fragments arranged by size:
860 254 143
BtsI GCAGTG_nn'
Cuts at: 0 520 1257
Size: 520 737
DraI TTT'AAA
Cuts at: 0 456 1257
Size: 456 801
EaeI y'GGCC_r
Cuts at: 0 1156 1257
Size: 1156 101
EarI CTCTTCn'nnn_
Cuts at: 0 305 857 973 1257
Size: 305 552 116 284
Fragments arranged by size:
552 305 284 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 824 1257
Size: 187 637 433
Fragments arranged by size:
637 433 187
EcoNI CCTnn'n_nnAGG
Cuts at: 0 772 1257
Size: 772 485
EcoO109I rG'GnC_Cy
Cuts at: 0 61 583 1257
Size: 61 522 674
Fragments arranged by size:
674 522 61
HaeII r_GCGC'y
Cuts at: 0 76 1257
Size: 76 1181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 95 127 922 954 1257
Size: 95 32 795 32 303
Fragments arranged by size:
795 303 95 32 32
KasI G'GCGC_C
Cuts at: 0 72 1257
Size: 72 1185
MslI CAynn'nnrTG
Cuts at: 0 945 1017 1101 1257
Size: 945 72 84 156
Fragments arranged by size:
945 156 84 72
MspA1I CmG'CkG
Cuts at: 0 46 908 1145 1257
Size: 46 862 237 112
Fragments arranged by size:
862 237 112 46
NarI GG'CG_CC
Cuts at: 0 73 1257
Size: 73 1184
NruI TCG'CGA
Cuts at: 0 68 439 1257
Size: 68 371 818
Fragments arranged by size:
818 371 68
NsiI A_TGCA'T
Cuts at: 0 394 634 1257
Size: 394 240 623
Fragments arranged by size:
623 394 240
NspI r_CATG'y
Cuts at: 0 396 636 744 1016 1142 1257
Size: 396 240 108 272 126 115
Fragments arranged by size:
396 272 240 126 115 108
PciI A'CATG_T
Cuts at: 0 740 1257
Size: 740 517
PfoI T'CCnGG_A
Cuts at: 0 1062 1257
Size: 1062 195
PpuMI rG'GwC_Cy
Cuts at: 0 61 1257
Size: 61 1196
PsiI TTA'TAA
Cuts at: 0 341 1257
Size: 341 916
PstI C_TGCA'G
Cuts at: 0 333 515 763 886 1257
Size: 333 182 248 123 371
Fragments arranged by size:
371 333 248 182 123
PvuII CAG'CTG
Cuts at: 0 908 1145 1257
Size: 908 237 112
Fragments arranged by size:
908 237 112
SfcI C'TryA_G
Cuts at: 0 329 477 511 759 882 1257
Size: 329 148 34 248 123 375
Fragments arranged by size:
375 329 248 148 123 34
SfoI GGC'GCC
Cuts at: 0 74 1257
Size: 74 1183
SmaI CCC'GGG
Cuts at: 0 59 1257
Size: 59 1198
SmlI C'TyrA_G
Cuts at: 0 300 578 1257
Size: 300 278 679
Fragments arranged by size:
679 300 278
SphI G_CATG'C
Cuts at: 0 396 1257
Size: 396 861
SspI AAT'ATT
Cuts at: 0 229 1257
Size: 229 1028
TatI w'GTAC_w
Cuts at: 0 213 239 1084 1257
Size: 213 26 845 173
Fragments arranged by size:
845 213 173 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 835 1257
Size: 835 422
XmaI C'CCGG_G
Cuts at: 0 57 1257
Size: 57 1200
Enzymes that do cut:
AccI AflIII ApoI AvaI BanI BanII BbeI BbvCI
BfrBI BglII BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI Bsp1286I BspEI BspHI BspMI
BsrBI BsrGI BssSI BstXI BstYI BtgI BtsI DraI
EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
MslI MspA1I NarI NruI NsiI NspI PciI PfoI
PpuMI PsiI PstI PvuII SfcI SfoI SmaI SmlI
SphI SspI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BclI BglI BlpI Bme1580I BmgBI BmrI BmtI BsaAI
BsaBI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BsrDI
BsrFI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI NaeI NcoI NdeI
NgoMIV NheI NotI PacI PasI PflMI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SrfI StuI StyI SwaI
TaqII TstI Tth111I XbaI XhoI XmnI ZraI