Restriction map for
pFN21AE1423
(Linear) (Six-base) MAPSORT of: KIEE1423.seq Check: 7326 from: 1 to: 1344
KIEE1423.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 25 1344
Size: 25 1319
AccI GT'mk_AC
Cuts at: 0 532 1344
Size: 532 812
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 1344
Size: 379 965
AflIII A'CryG_T
Cuts at: 0 180 1344
Size: 180 1164
AleI CACnn'nnGTG
Cuts at: 0 1084 1344
Size: 1084 260
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1165 1197 1234 1266 1344
Size: 1165 32 37 32 78
Fragments arranged by size:
1165 78 37 32 32
ApaI G_GGCC'C
Cuts at: 0 741 1167 1344
Size: 741 426 177
Fragments arranged by size:
741 426 177
ApaLI G'TGCA_C
Cuts at: 0 384 1213 1344
Size: 384 829 131
Fragments arranged by size:
829 384 131
ApoI r'AATT_y
Cuts at: 0 502 1344
Size: 502 842
AvaI C'yCGr_G
Cuts at: 0 188 1344
Size: 188 1156
BamHI G'GATC_C
Cuts at: 0 362 1344
Size: 362 982
BanI G'GyrC_C
Cuts at: 0 777 969 1037 1086 1344
Size: 777 192 68 49 258
Fragments arranged by size:
777 258 192 68 49
BanII G_rGCy'C
Cuts at: 0 68 551 741 1167 1344
Size: 68 483 190 426 177
Fragments arranged by size:
483 426 190 177 68
BfrBI ATG'CAT
Cuts at: 0 11 1344
Size: 11 1333
BglI GCCn_nnn'nGGC
Cuts at: 0 99 1046 1204 1344
Size: 99 947 158 140
Fragments arranged by size:
947 158 140 99
BlpI GC'TnA_GC
Cuts at: 0 1132 1344
Size: 1132 212
Bme1580I G_kGCm'C
Cuts at: 0 388 433 741 782 974 1042 1167 1217
Size: 388 45 308 41 192 68 125 50
Cuts at: 1217 1344
Size: 127
Fragments arranged by size:
388 308 192 127 125 68 50 45
41
BmrI ACTGGGnnnn_n'
Cuts at: 0 104 290 343 1344
Size: 104 186 53 1001
Fragments arranged by size:
1001 186 104 53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 486 1344
Size: 486 858
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 604 1303 1344
Size: 229 375 699 41
Fragments arranged by size:
699 375 229 41
BsaAI yAC'GTr
Cuts at: 0 24 183 1344
Size: 24 159 1161
Fragments arranged by size:
1161 159 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 953 983 1234 1264 1344
Size: 953 30 251 30 80
Fragments arranged by size:
953 251 80 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 696 1255 1312 1344
Size: 696 559 57 32
Fragments arranged by size:
696 559 57 32
BseYI C'CCAG_C
Cuts at: 0 59 516 1344
Size: 59 457 828
Fragments arranged by size:
828 457 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 1344
Size: 1132 212
BsiEI CG_ry'CG
Cuts at: 0 1145 1344
Size: 1145 199
BsiHKAI G_wGCw'C
Cuts at: 0 68 388 590 1217 1344
Size: 68 320 202 627 127
Fragments arranged by size:
627 320 202 127 68
BsmI GAATG_Cn'
Cuts at: 0 13 1019 1344
Size: 13 1006 325
Fragments arranged by size:
1006 325 13
Bsp1286I G_dGCh'C
Cuts at: 0 68 388 433 551 590 741 782 974
Size: 68 320 45 118 39 151 41 192
Cuts at: 974 1042 1167 1217 1344
Size: 68 125 50 127
Fragments arranged by size:
320 192 151 127 125 118 68 68
50 45 41 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 25 1344
Size: 25 1319
BsrBI CCG'CTC
Cuts at: 0 465 1344
Size: 465 879
BsrDI GCAATG_nn'
Cuts at: 0 57 992 1344
Size: 57 935 352
Fragments arranged by size:
935 352 57
BssSI C'ACGA_G
Cuts at: 0 847 1344
Size: 847 497
BstAPI GCAn_nnn'nTGC
Cuts at: 0 56 1344
Size: 56 1288
BstEII G'GTnAC_C
Cuts at: 0 1096 1344
Size: 1096 248
BstXI CCAn_nnnn'nTGG
Cuts at: 0 421 903 1344
Size: 421 482 441
Fragments arranged by size:
482 441 421
BstYI r'GATC_y
Cuts at: 0 204 230 362 1344
Size: 204 26 132 982
Fragments arranged by size:
982 204 132 26
BtgI C'CryG_G
Cuts at: 0 312 539 957 1344
Size: 312 227 418 387
Fragments arranged by size:
418 387 312 227
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1134 1344
Size: 1134 210
EaeI y'GGCC_r
Cuts at: 0 806 1103 1344
Size: 806 297 241
Fragments arranged by size:
806 297 241
EarI CTCTTCn'nnn_
Cuts at: 0 262 1344
Size: 262 1082
EcoICRI GAG'CTC
Cuts at: 0 66 1344
Size: 66 1278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 379 604 1303 1344
Size: 229 150 225 699 41
Fragments arranged by size:
699 229 225 150 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1105 1285 1344
Size: 1105 180 59
Fragments arranged by size:
1105 180 59
EcoO109I rG'GnC_Cy
Cuts at: 0 737 738 1344
Size: 737 1 606
Fragments arranged by size:
737 606 1
EcoRI G'AATT_C
Cuts at: 0 502 1344
Size: 502 842
HaeII r_GCGC'y
Cuts at: 0 663 1344
Size: 663 681
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 841 873 1344
Size: 841 32 471
Fragments arranged by size:
841 471 32
HincII GTy'rAC
Cuts at: 0 477 984 1344
Size: 477 507 360
Fragments arranged by size:
507 477 360
MscI TGG'CCA
Cuts at: 0 808 1105 1344
Size: 808 297 239
Fragments arranged by size:
808 297 239
MslI CAynn'nnrTG
Cuts at: 0 1084 1220 1344
Size: 1084 136 124
Fragments arranged by size:
1084 136 124
MspA1I CmG'CkG
Cuts at: 0 855 1344
Size: 855 489
NsiI A_TGCA'T
Cuts at: 0 13 1344
Size: 13 1331
NspI r_CATG'y
Cuts at: 0 1131 1344
Size: 1131 213
PmlI CAC'GTG
Cuts at: 0 24 1344
Size: 24 1320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1234 1266 1344
Size: 1234 32 78
Fragments arranged by size:
1234 78 32
PspOMI G'GGCC_C
Cuts at: 0 737 1163 1344
Size: 737 426 181
Fragments arranged by size:
737 426 181
PstI C_TGCA'G
Cuts at: 0 834 1344
Size: 834 510
PvuII CAG'CTG
Cuts at: 0 855 1344
Size: 855 489
SacI G_AGCT'C
Cuts at: 0 68 1344
Size: 68 1276
SapI GCTCTTCn'nnn_
Cuts at: 0 262 1344
Size: 262 1082
SexAI A'CCwGG_T
Cuts at: 0 773 1344
Size: 773 571
SfcI C'TryA_G
Cuts at: 0 533 830 1344
Size: 533 297 514
Fragments arranged by size:
533 514 297
SmaI CCC'GGG
Cuts at: 0 190 1344
Size: 190 1154
SphI G_CATG'C
Cuts at: 0 1131 1344
Size: 1131 213
StuI AGG'CCT
Cuts at: 0 1159 1344
Size: 1159 185
StyI C'CwwG_G
Cuts at: 0 974 1106 1344
Size: 974 132 238
Fragments arranged by size:
974 238 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 278 586 1216 1344
Size: 278 308 630 128
Fragments arranged by size:
630 308 278 128
TatI w'GTAC_w
Cuts at: 0 932 1344
Size: 932 412
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 953 985 1344
Size: 953 32 359
Fragments arranged by size:
953 359 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 421 1344
Size: 421 923
XmaI C'CCGG_G
Cuts at: 0 188 1344
Size: 188 1156
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AloI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BfrBI BglI BlpI
Bme1580I BmrI BplI BpmI BsaAI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HincII MscI MslI MspA1I NsiI NspI PmlI PpiI
PspOMI PstI PvuII SacI SapI SexAI SfcI SmaI
SphI StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AlwNI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BbvCI BcgI BciVI BclI BglII BmgBI BmtI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsiWI BsmBI BspEI
BspHI BsrFI BsrGI BssHII BstBI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PfoI
PmeI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XhoI XmnI ZraI