Restriction map for
pFN21AE1421
(Linear) (Six-base) MAPSORT of: KIEE1421.seq Check: 5646 from: 1 to: 1446
KIEE1421.orf
With 182 enzymes: SgfI *
July 3, 2012 16:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 399 1446
Size: 399 1047
AccI GT'mk_AC
Cuts at: 0 1274 1446
Size: 1274 172
AfeI AGC'GCT
Cuts at: 0 806 1446
Size: 806 640
AflII C'TTAA_G
Cuts at: 0 903 1446
Size: 903 543
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 426 1446
Size: 426 1020
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 688 720 1446
Size: 688 32 726
Fragments arranged by size:
726 688 32
AlwNI CAG_nnn'CTG
Cuts at: 0 282 303 1113 1290 1446
Size: 282 21 810 177 156
Fragments arranged by size:
810 282 177 156 21
ApaI G_GGCC'C
Cuts at: 0 33 1446
Size: 33 1413
ApaLI G'TGCA_C
Cuts at: 0 1318 1446
Size: 1318 128
AvaI C'yCGr_G
Cuts at: 0 18 743 925 983 1070 1083 1124 1210
Size: 18 725 182 58 87 13 41 86
Cuts at: 1210 1446
Size: 236
Fragments arranged by size:
725 236 182 87 86 58 41 18
13
AvrII C'CTAG_G
Cuts at: 0 783 1446
Size: 783 663
BamHI G'GATC_C
Cuts at: 0 1056 1446
Size: 1056 390
BanI G'GyrC_C
Cuts at: 0 323 417 457 931 1146 1159 1446
Size: 323 94 40 474 215 13 287
Fragments arranged by size:
474 323 287 215 94 40 13
BanII G_rGCy'C
Cuts at: 0 33 98 508 1077 1211 1427 1446
Size: 33 65 410 569 134 216 19
Fragments arranged by size:
569 410 216 134 65 33 19
BbeI G_GCGC'C
Cuts at: 0 327 935 1150 1446
Size: 327 608 215 296
Fragments arranged by size:
608 327 296 215
BbsI GAAGACnn'nnnn_
Cuts at: 0 714 1446
Size: 714 732
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1061 1095 1446
Size: 1061 34 351
Fragments arranged by size:
1061 351 34
BclI T'GATC_A
Cuts at: 0 425 1446
Size: 425 1021
BglI GCCn_nnn'nGGC
Cuts at: 0 400 988 1446
Size: 400 588 458
Fragments arranged by size:
588 458 400
BlpI GC'TnA_GC
Cuts at: 0 946 1339 1446
Size: 946 393 107
Fragments arranged by size:
946 393 107
Bme1580I G_kGCm'C
Cuts at: 0 33 449 1322 1446
Size: 33 416 873 124
Fragments arranged by size:
873 416 124 33
BmgBI CAC'GTC
Cuts at: 0 436 1278 1446
Size: 436 842 168
Fragments arranged by size:
842 436 168
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 279 1446
Size: 279 1167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 325 493 874 1446
Size: 274 51 168 381 572
Fragments arranged by size:
572 381 274 168 51
Bpu10I CC'TnA_GC
Cuts at: 0 145 971 1446
Size: 145 826 475
Fragments arranged by size:
826 475 145
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 1446
Size: 253 1193
BsaBI GATnn'nnATC
Cuts at: 0 229 1446
Size: 229 1217
BsaHI Gr'CG_yC
Cuts at: 0 324 932 1147 1446
Size: 324 608 215 299
Fragments arranged by size:
608 324 299 215
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 664 694 1446
Size: 664 30 752
Fragments arranged by size:
752 664 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 877 1150 1446
Size: 271 606 273 296
Fragments arranged by size:
606 296 273 271
BseYI C'CCAG_C
Cuts at: 0 1231 1446
Size: 1231 215
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1186 1446
Size: 1186 260
BsiEI CG_ry'CG
Cuts at: 0 1389 1446
Size: 1389 57
BsiHKAI G_wGCw'C
Cuts at: 0 1084 1211 1322 1427 1446
Size: 1084 127 111 105 19
Fragments arranged by size:
1084 127 111 105 19
BsmI GAATG_Cn'
Cuts at: 0 345 1446
Size: 345 1101
BsmBI CGTCTCn'nnnn_
Cuts at: 0 125 1446
Size: 125 1321
Bsp1286I G_dGCh'C
Cuts at: 0 33 98 449 508 1077 1084 1211 1322
Size: 33 65 351 59 569 7 127 111
Cuts at: 1322 1427 1446
Size: 105 19
Fragments arranged by size:
569 351 127 111 105 65 59 33
19 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 399 1446
Size: 399 1047
BsrBI CCG'CTC
Cuts at: 0 685 1032 1242 1446
Size: 685 347 210 204
Fragments arranged by size:
685 347 210 204
BsrFI r'CCGG_y
Cuts at: 0 1103 1446
Size: 1103 343
BssHII G'CGCG_C
Cuts at: 0 194 1391 1446
Size: 194 1197 55
Fragments arranged by size:
1197 194 55
BssSI C'ACGA_G
Cuts at: 0 667 1204 1446
Size: 667 537 242
Fragments arranged by size:
667 537 242
BstAPI GCAn_nnn'nTGC
Cuts at: 0 525 1113 1446
Size: 525 588 333
Fragments arranged by size:
588 525 333
BstEII G'GTnAC_C
Cuts at: 0 915 1446
Size: 915 531
BstXI CCAn_nnnn'nTGG
Cuts at: 0 225 320 1446
Size: 225 95 1126
Fragments arranged by size:
1126 225 95
BstYI r'GATC_y
Cuts at: 0 249 1056 1446
Size: 249 807 390
Fragments arranged by size:
807 390 249
BtgI C'CryG_G
Cuts at: 0 620 815 992 1163 1296 1446
Size: 620 195 177 171 133 150
Fragments arranged by size:
620 195 177 171 150 133
EaeI y'GGCC_r
Cuts at: 0 173 227 310 989 1020 1446
Size: 173 54 83 679 31 426
Fragments arranged by size:
679 426 173 83 54 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1234 1446
Size: 1234 212
EcoICRI GAG'CTC
Cuts at: 0 1209 1425 1446
Size: 1209 216 21
Fragments arranged by size:
1209 216 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 325 493 874 1446
Size: 274 51 168 381 572
Fragments arranged by size:
572 381 274 168 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1366 1446
Size: 1366 80
EcoO109I rG'GnC_Cy
Cuts at: 0 29 30 279 909 1301 1446
Size: 29 1 249 630 392 145
Fragments arranged by size:
630 392 249 145 29 1
HaeII r_GCGC'y
Cuts at: 0 327 808 871 935 980 1000 1150 1183
Size: 327 481 63 64 45 20 150 33
Cuts at: 1183 1195 1375 1446
Size: 12 180 71
Fragments arranged by size:
481 327 180 150 71 64 63 45
33 20 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 479 511 664 696 954 986 994 1026
Size: 479 32 153 32 258 32 8 32
Cuts at: 1026 1446
Size: 420
Fragments arranged by size:
479 420 258 153 32 32 32 32
8
HincII GTy'rAC
Cuts at: 0 1275 1446
Size: 1275 171
KasI G'GCGC_C
Cuts at: 0 323 931 1146 1446
Size: 323 608 215 300
Fragments arranged by size:
608 323 300 215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1409 1446
Size: 258 1151 37
Fragments arranged by size:
1151 258 37
MscI TGG'CCA
Cuts at: 0 229 312 1446
Size: 229 83 1134
Fragments arranged by size:
1134 229 83
MslI CAynn'nnrTG
Cuts at: 0 223 1446
Size: 223 1223
MspA1I CmG'CkG
Cuts at: 0 178 303 431 570 950 994 1110 1188
Size: 178 125 128 139 380 44 116 78
Cuts at: 1188 1343 1446
Size: 155 103
Fragments arranged by size:
380 178 155 139 128 125 116 103
78 44
NaeI GCC'GGC
Cuts at: 0 1105 1446
Size: 1105 341
NarI GG'CG_CC
Cuts at: 0 324 932 1147 1446
Size: 324 608 215 299
Fragments arranged by size:
608 324 299 215
NcoI C'CATG_G
Cuts at: 0 815 1446
Size: 815 631
NgoMIV G'CCGG_C
Cuts at: 0 1103 1446
Size: 1103 343
NspI r_CATG'y
Cuts at: 0 407 1446
Size: 407 1039
PasI CC'CwG_GG
Cuts at: 0 828 1446
Size: 828 618
PflMI CCAn_nnn'nTGG
Cuts at: 0 282 852 1446
Size: 282 570 594
Fragments arranged by size:
594 570 282
PfoI T'CCnGG_A
Cuts at: 0 107 483 1446
Size: 107 376 963
Fragments arranged by size:
963 376 107
PpuMI rG'GwC_Cy
Cuts at: 0 909 1301 1446
Size: 909 392 145
Fragments arranged by size:
909 392 145
PspOMI G'GGCC_C
Cuts at: 0 29 1446
Size: 29 1417
PstI C_TGCA'G
Cuts at: 0 1367 1446
Size: 1367 79
PvuII CAG'CTG
Cuts at: 0 303 570 1110 1446
Size: 303 267 540 336
Fragments arranged by size:
540 336 303 267
SacI G_AGCT'C
Cuts at: 0 1211 1427 1446
Size: 1211 216 19
Fragments arranged by size:
1211 216 19
SacII CC_GC'GG
Cuts at: 0 995 1446
Size: 995 451
SalI G'TCGA_C
Cuts at: 0 1273 1446
Size: 1273 173
SanDI GG'GwC_CC
Cuts at: 0 1301 1446
Size: 1301 145
SbfI CC_TGCA'GG
Cuts at: 0 1367 1446
Size: 1367 79
SexAI A'CCwGG_T
Cuts at: 0 911 1446
Size: 911 535
SfcI C'TryA_G
Cuts at: 0 1363 1446
Size: 1363 83
SfoI GGC'GCC
Cuts at: 0 325 933 1148 1446
Size: 325 608 215 298
Fragments arranged by size:
608 325 298 215
SmaI CCC'GGG
Cuts at: 0 20 745 985 1126 1446
Size: 20 725 240 141 320
Fragments arranged by size:
725 320 240 141 20
SmlI C'TyrA_G
Cuts at: 0 903 1446
Size: 903 543
StuI AGG'CCT
Cuts at: 0 319 1446
Size: 319 1127
StyI C'CwwG_G
Cuts at: 0 461 783 815 1017 1446
Size: 461 322 32 202 429
Fragments arranged by size:
461 429 322 202 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 352 1446
Size: 352 1094
Tth111I GACn'n_nGTC
Cuts at: 0 370 437 913 1446
Size: 370 67 476 533
Fragments arranged by size:
533 476 370 67
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 357 1446
Size: 357 1089
XmaI C'CCGG_G
Cuts at: 0 18 743 983 1124 1446
Size: 18 725 240 141 322
Fragments arranged by size:
725 322 240 141 18
Enzymes that do cut:
AarI AccI AfeI AflII AlfI AloI AlwNI ApaI
ApaLI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BclI BglI BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI BtgI EaeI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SacII SalI SanDI SbfI SexAI SfcI
SfoI SmaI SmlI StuI StyI TaqII Tth111I XcmI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflIII AgeI AhdI AleI
ApoI AscI AseI AsiSI BaeI BbvCI BciVI BfrBI
BglII BmrI BmtI BpuEI BsaAI BsaWI BsiWI BspEI
BspHI BsrDI BsrGI BstBI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EarI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SapI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI XbaI
XhoI XmnI ZraI