Restriction map for
pFN21AE1420
(Linear) (Six-base) MAPSORT of: KIEE1420.seq Check: 9902 from: 1 to: 627
KIEE1420.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 443 627
Size: 443 184
Acc65I G'GTAC_C
Cuts at: 0 140 627
Size: 140 487
ApaI G_GGCC'C
Cuts at: 0 327 627
Size: 327 300
AvaI C'yCGr_G
Cuts at: 0 473 627
Size: 473 154
BanI G'GyrC_C
Cuts at: 0 140 627
Size: 140 487
BanII G_rGCy'C
Cuts at: 0 229 327 568 627
Size: 229 98 241 59
Fragments arranged by size:
241 229 98 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 52 627
Size: 52 575
BciVI GTATCCnnnnn_n'
Cuts at: 0 385 627
Size: 385 242
BglI GCCn_nnn'nGGC
Cuts at: 0 79 627
Size: 79 548
Bme1580I G_kGCm'C
Cuts at: 0 327 627
Size: 327 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 202 627
Size: 84 118 425
Fragments arranged by size:
425 118 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 627
Size: 403 224
BsaI GGTCTCn'nnnn_
Cuts at: 0 7 627
Size: 7 620
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 558 627
Size: 558 69
BseYI C'CCAG_C
Cuts at: 0 66 161 548 613 627
Size: 66 95 387 65 14
Fragments arranged by size:
387 95 66 65 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 452 627
Size: 415 37 175
Fragments arranged by size:
415 175 37
BsiHKAI G_wGCw'C
Cuts at: 0 29 627
Size: 29 598
Bsp1286I G_dGCh'C
Cuts at: 0 29 229 327 568 627
Size: 29 200 98 241 59
Fragments arranged by size:
241 200 98 59 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 443 627
Size: 443 184
Bsu36I CC'TnA_GG
Cuts at: 0 353 627
Size: 353 274
BtgI C'CryG_G
Cuts at: 0 417 627
Size: 417 210
EaeI y'GGCC_r
Cuts at: 0 313 627
Size: 313 314
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 202 627
Size: 84 118 425
Fragments arranged by size:
425 118 84
EcoO109I rG'GnC_Cy
Cuts at: 0 323 324 462 592 627
Size: 323 1 138 130 35
Fragments arranged by size:
323 138 130 35 1
HaeII r_GCGC'y
Cuts at: 0 215 627
Size: 215 412
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 627
Size: 550 32 45
Fragments arranged by size:
550 45 32
KpnI G_GTAC'C
Cuts at: 0 144 627
Size: 144 483
MspA1I CmG'CkG
Cuts at: 0 138 246 312 429 627
Size: 138 108 66 117 198
Fragments arranged by size:
198 138 117 108 66
PasI CC'CwG_GG
Cuts at: 0 600 627
Size: 600 27
PfoI T'CCnGG_A
Cuts at: 0 368 627
Size: 368 259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 627
Size: 4 32 591
Fragments arranged by size:
591 32 4
PpuMI rG'GwC_Cy
Cuts at: 0 462 592 627
Size: 462 130 35
Fragments arranged by size:
462 130 35
PshAI GACnn'nnGTC
Cuts at: 0 101 627
Size: 101 526
PspOMI G'GGCC_C
Cuts at: 0 323 627
Size: 323 304
PstI C_TGCA'G
Cuts at: 0 245 364 627
Size: 245 119 263
Fragments arranged by size:
263 245 119
PvuII CAG'CTG
Cuts at: 0 246 627
Size: 246 381
SanDI GG'GwC_CC
Cuts at: 0 462 627
Size: 462 165
SfcI C'TryA_G
Cuts at: 0 241 360 627
Size: 241 119 267
Fragments arranged by size:
267 241 119
SmlI C'TyrA_G
Cuts at: 0 382 627
Size: 382 245
StuI AGG'CCT
Cuts at: 0 358 506 627
Size: 358 148 121
Fragments arranged by size:
358 148 121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 74 106 521 553 627
Size: 74 32 415 32 74
Fragments arranged by size:
415 74 74 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 316 627
Size: 316 311
Enzymes that do cut:
AarI Acc65I ApaI AvaI BanI BanII BbsI BciVI
BglI Bme1580I BpmI BpuEI BsaI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI Bsu36I BtgI EaeI Eco57MI EcoO109I
HaeII Hin4I KpnI MspA1I PasI PfoI PpiI PpuMI
PshAI PspOMI PstI PvuII SanDI SfcI SmlI StuI
TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI MfeI MluI
MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StyI SwaI TaqII TatI Tth111I XbaI XhoI XmaI
XmnI ZraI