Restriction map for
pFN21AE1418
(Linear) (Six-base) MAPSORT of: KIEE1418.seq Check: 7531 from: 1 to: 1227
KIEE1418.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 235 342 1227
Size: 213 22 107 885
Fragments arranged by size:
885 213 107 22
AflIII A'CryG_T
Cuts at: 0 171 725 979 1064 1227
Size: 171 554 254 85 163
Fragments arranged by size:
554 254 171 163 85
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1103 1227
Size: 1103 124
ApoI r'AATT_y
Cuts at: 0 319 1227
Size: 319 908
AvaI C'yCGr_G
Cuts at: 0 97 1227
Size: 97 1130
BamHI G'GATC_C
Cuts at: 0 968 1227
Size: 968 259
BanI G'GyrC_C
Cuts at: 0 1054 1227
Size: 1054 173
BanII G_rGCy'C
Cuts at: 0 412 1227
Size: 412 815
BbeI G_GCGC'C
Cuts at: 0 1058 1227
Size: 1058 169
BciVI GTATCCnnnnn_n'
Cuts at: 0 922 1227
Size: 922 305
BclI T'GATC_A
Cuts at: 0 425 1227
Size: 425 802
BglI GCCn_nnn'nGGC
Cuts at: 0 532 1031 1081 1227
Size: 532 499 50 146
Fragments arranged by size:
532 499 146 50
Bme1580I G_kGCm'C
Cuts at: 0 283 853 1227
Size: 283 570 374
Fragments arranged by size:
570 374 283
BmgBI CAC'GTC
Cuts at: 0 1067 1227
Size: 1067 160
BmrI ACTGGGnnnn_n'
Cuts at: 0 1102 1227
Size: 1102 125
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 814 1227
Size: 814 413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 716 756 854 1227
Size: 256 460 40 98 373
Fragments arranged by size:
460 373 256 98 40
BsaI GGTCTCn'nnnn_
Cuts at: 0 921 1227
Size: 921 306
BsaAI yAC'GTr
Cuts at: 0 728 1227
Size: 728 499
BsaHI Gr'CG_yC
Cuts at: 0 1055 1227
Size: 1055 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 830 860 1227
Size: 830 30 367
Fragments arranged by size:
830 367 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 851 1227
Size: 851 376
BseYI C'CCAG_C
Cuts at: 0 667 1227
Size: 667 560
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 729 1073 1227
Size: 383 346 344 154
Fragments arranged by size:
383 346 344 154
BsiHKAI G_wGCw'C
Cuts at: 0 755 788 1227
Size: 755 33 439
Fragments arranged by size:
755 439 33
BsmI GAATG_Cn'
Cuts at: 0 825 1227
Size: 825 402
Bsp1286I G_dGCh'C
Cuts at: 0 283 412 755 788 853 1227
Size: 283 129 343 33 65 374
Fragments arranged by size:
374 343 283 129 65 33
BspHI T'CATG_A
Cuts at: 0 203 428 1227
Size: 203 225 799
Fragments arranged by size:
799 225 203
BsrDI GCAATG_nn'
Cuts at: 0 638 683 902 1055 1227
Size: 638 45 219 153 172
Fragments arranged by size:
638 219 172 153 45
BsrGI T'GTAC_A
Cuts at: 0 168 288 1227
Size: 168 120 939
Fragments arranged by size:
939 168 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 529 780 1227
Size: 529 251 447
Fragments arranged by size:
529 447 251
BstYI r'GATC_y
Cuts at: 0 968 1227
Size: 968 259
BtgI C'CryG_G
Cuts at: 0 999 1227
Size: 999 228
BtsI GCAGTG_nn'
Cuts at: 0 240 1227
Size: 240 987
ClaI AT'CG_AT
Cuts at: 0 570 1227
Size: 570 657
EaeI y'GGCC_r
Cuts at: 0 680 1073 1227
Size: 680 393 154
Fragments arranged by size:
680 393 154
EarI CTCTTCn'nnn_
Cuts at: 0 596 1227
Size: 596 631
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 235 256 342 716 756 854 1227
Size: 213 22 21 86 374 40 98 373
Fragments arranged by size:
374 373 213 98 86 40 22 21
EcoO109I rG'GnC_Cy
Cuts at: 0 533 1212 1227
Size: 533 679 15
Fragments arranged by size:
679 533 15
EcoRV GAT'ATC
Cuts at: 0 380 1227
Size: 380 847
HaeII r_GCGC'y
Cuts at: 0 1058 1227
Size: 1058 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 627 659 1227
Size: 627 32 568
Fragments arranged by size:
627 568 32
KasI G'GCGC_C
Cuts at: 0 1054 1227
Size: 1054 173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1085 1101 1227
Size: 1085 16 126
Fragments arranged by size:
1085 126 16
MscI TGG'CCA
Cuts at: 0 1075 1227
Size: 1075 152
MslI CAynn'nnrTG
Cuts at: 0 418 1069 1227
Size: 418 651 158
Fragments arranged by size:
651 418 158
MspA1I CmG'CkG
Cuts at: 0 1087 1227
Size: 1087 140
NarI GG'CG_CC
Cuts at: 0 1055 1227
Size: 1055 172
NspI r_CATG'y
Cuts at: 0 175 983 1227
Size: 175 808 244
Fragments arranged by size:
808 244 175
PciI A'CATG_T
Cuts at: 0 171 979 1227
Size: 171 808 248
Fragments arranged by size:
808 248 171
PfoI T'CCnGG_A
Cuts at: 0 32 1227
Size: 32 1195
PpuMI rG'GwC_Cy
Cuts at: 0 1212 1227
Size: 1212 15
PvuII CAG'CTG
Cuts at: 0 1087 1227
Size: 1087 140
SexAI A'CCwGG_T
Cuts at: 0 470 1008 1227
Size: 470 538 219
Fragments arranged by size:
538 470 219
SfcI C'TryA_G
Cuts at: 0 26 551 1227
Size: 26 525 676
Fragments arranged by size:
676 525 26
SfoI GGC'GCC
Cuts at: 0 1056 1227
Size: 1056 171
SmlI C'TyrA_G
Cuts at: 0 97 1227
Size: 97 1130
SspI AAT'ATT
Cuts at: 0 485 1227
Size: 485 742
StuI AGG'CCT
Cuts at: 0 493 1025 1227
Size: 493 532 202
Fragments arranged by size:
532 493 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 430 890 1227
Size: 430 460 337
Fragments arranged by size:
460 430 337
TatI w'GTAC_w
Cuts at: 0 168 288 1227
Size: 168 120 939
Fragments arranged by size:
939 168 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 830 862 1227
Size: 830 32 365
Fragments arranged by size:
830 365 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 979 1006 1068 1227
Size: 979 27 62 159
Fragments arranged by size:
979 159 62 27
XhoI C'TCGA_G
Cuts at: 0 97 1227
Size: 97 1130
Enzymes that do cut:
AcuI AflIII AlfI ApoI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI Bme1580I BmgBI BmrI BplI
BpmI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BstXI BstYI
BtgI BtsI ClaI EaeI EarI Eco57MI EcoO109I EcoRV
HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI
NspI PciI PfoI PpuMI PvuII SexAI SfcI SfoI
SmlI SspI StuI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbsI BbvCI BcgI BfrBI BglII
BlpI BmtI Bpu10I BpuEI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StyI SwaI Tth111I
XbaI XmaI XmnI ZraI