Restriction map for
pFN21AE1414
(Linear) (Six-base) MAPSORT of: KIEE1414.seq Check: 1801 from: 1 to: 672
KIEE1414.orf
With 182 enzymes: SgfI *
July 3, 2012 16:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 493 619 641 672
Size: 493 126 22 31
Fragments arranged by size:
493 126 31 22
Acc65I G'GTAC_C
Cuts at: 0 620 672
Size: 620 52
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 108 672
Size: 108 564
AlwNI CAG_nnn'CTG
Cuts at: 0 190 672
Size: 190 482
AvaI C'yCGr_G
Cuts at: 0 69 672
Size: 69 603
BanI G'GyrC_C
Cuts at: 0 607 620 672
Size: 607 13 52
Fragments arranged by size:
607 52 13
BanII G_rGCy'C
Cuts at: 0 13 407 672
Size: 13 394 265
Fragments arranged by size:
394 265 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 369 672
Size: 369 303
BbvCI CC'TCA_GC
Cuts at: 0 110 672
Size: 110 562
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 381 415 672
Size: 381 34 257
Fragments arranged by size:
381 257 34
BglI GCCn_nnn'nGGC
Cuts at: 0 72 672
Size: 72 600
BlpI GC'TnA_GC
Cuts at: 0 575 672
Size: 575 97
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 672
Size: 141 531
Bpu10I CC'TnA_GC
Cuts at: 0 110 672
Size: 110 562
BsaWI w'CCGG_w
Cuts at: 0 212 672
Size: 212 460
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 403 433 672
Size: 403 30 239
Fragments arranged by size:
403 239 30
BseYI C'CCAG_C
Cuts at: 0 29 75 672
Size: 29 46 597
Fragments arranged by size:
597 46 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 448 672
Size: 203 245 224
Fragments arranged by size:
245 224 203
Bsp1286I G_dGCh'C
Cuts at: 0 13 407 672
Size: 13 394 265
Fragments arranged by size:
394 265 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 493 619 641 672
Size: 493 126 22 31
Fragments arranged by size:
493 126 31 22
BstAPI GCAn_nnn'nTGC
Cuts at: 0 190 672
Size: 190 482
BstEII G'GTnAC_C
Cuts at: 0 565 672
Size: 565 107
EaeI y'GGCC_r
Cuts at: 0 176 672
Size: 176 496
EarI CTCTTCn'nnn_
Cuts at: 0 300 672
Size: 300 372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 672
Size: 141 531
EcoO109I rG'GnC_Cy
Cuts at: 0 357 414 672
Size: 357 57 258
Fragments arranged by size:
357 258 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 403 423 435 455 672
Size: 403 20 12 20 217
Fragments arranged by size:
403 217 20 20 12
HincII GTy'rAC
Cuts at: 0 603 657 672
Size: 603 54 15
Fragments arranged by size:
603 54 15
KpnI G_GTAC'C
Cuts at: 0 624 672
Size: 624 48
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 672
Size: 527 145
MspA1I CmG'CkG
Cuts at: 0 92 617 672
Size: 92 525 55
Fragments arranged by size:
525 92 55
NspI r_CATG'y
Cuts at: 0 89 601 672
Size: 89 512 71
Fragments arranged by size:
512 89 71
PasI CC'CwG_GG
Cuts at: 0 407 672
Size: 407 265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 542 574 672
Size: 542 32 98
Fragments arranged by size:
542 98 32
PpuMI rG'GwC_Cy
Cuts at: 0 357 414 672
Size: 357 57 258
Fragments arranged by size:
357 258 57
PstI C_TGCA'G
Cuts at: 0 616 672
Size: 616 56
PvuII CAG'CTG
Cuts at: 0 92 617 672
Size: 92 525 55
Fragments arranged by size:
525 92 55
SfcI C'TryA_G
Cuts at: 0 612 672
Size: 612 60
SmaI CCC'GGG
Cuts at: 0 71 672
Size: 71 601
SphI G_CATG'C
Cuts at: 0 89 672
Size: 89 583
StyI C'CwwG_G
Cuts at: 0 422 672
Size: 422 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 67 672
Size: 21 46 605
Fragments arranged by size:
605 46 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 463 672
Size: 463 209
XmaI C'CCGG_G
Cuts at: 0 69 672
Size: 69 603
Enzymes that do cut:
AarI Acc65I AlfI AlwNI AvaI BanI BanII BbsI
BbvCI BcgI BglI BlpI BpmI Bpu10I BsaWI BsaXI
BseYI BsgI Bsp1286I BspMI BstAPI BstEII EaeI EarI
Eco57MI EcoO109I Hin4I HincII KpnI MmeI MspA1I NspI
PasI PpiI PpuMI PstI PvuII SfcI SmaI SphI
StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI
BglII Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BseRI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI MfeI MluI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI