(Linear) (Six-base) MAPSORT of: KIEE1412.seq  Check: 829  from: 1  to: 447



KIEE1412.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:38  ..

AflIII A'CryG_T

Cuts at:      0     321     447 
   Size:        321     126
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     124     447 
   Size:        124     323
 
ApoI r'AATT_y

Cuts at:      0      34     447 
   Size:         34     413
 
AvaI C'yCGr_G

Cuts at:      0     199     447 
   Size:        199     248
 
BanI G'GyrC_C

Cuts at:      0     184     447 
   Size:        184     263
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     279     313     447 
   Size:        279      34     134

  Fragments arranged by size: 

                 279     134      34
 
Bme1580I G_kGCm'C

Cuts at:      0     103     447 
   Size:        103     344
 
BmgBI CAC'GTC

Cuts at:      0     324     447 
   Size:        324     123
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      66     447 
   Size:         66     381
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34     262     265     373     433     447 
   Size:         34     228       3     108      60      14

  Fragments arranged by size: 

                 228     108      60      34      14       3
 
BseYI C'CCAG_C

Cuts at:      0      96     336     447 
   Size:         96     240     111

  Fragments arranged by size: 

                 240     111      96
 
BsiEI CG_ry'CG

Cuts at:      0     171     447 
   Size:        171     276
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     392     447 
   Size:        392      55
 
Bsp1286I G_dGCh'C

Cuts at:      0     103     447 
   Size:        103     344
 
BspHI T'CATG_A

Cuts at:      0     326     447 
   Size:        326     121
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      15     447 
   Size:         15     432
 
BstYI r'GATC_y

Cuts at:      0     254     447 
   Size:        254     193
 
BtgI C'CryG_G

Cuts at:      0     131     188     383     447 
   Size:        131      57     195      64

  Fragments arranged by size: 

                 195     131      64      57
 
EaeI y'GGCC_r

Cuts at:      0       2     218     447 
   Size:          2     216     229

  Fragments arranged by size: 

                 229     216       2
 
EcoRI G'AATT_C

Cuts at:      0      34     447 
   Size:         34     413
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      63     447 
   Size:         63     384
 
HaeII r_GCGC'y

Cuts at:      0     308     447 
   Size:        308     139
 
MscI TGG'CCA

Cuts at:      0     220     447 
   Size:        220     227
 
MslI CAynn'nnrTG

Cuts at:      0     106     447 
   Size:        106     341
 
MspA1I CmG'CkG

Cuts at:      0      12     385     447 
   Size:         12     373      62

  Fragments arranged by size: 

                 373      62      12
 
PasI CC'CwG_GG

Cuts at:      0     117     447 
   Size:        117     330
 
PvuII CAG'CTG

Cuts at:      0      12     447 
   Size:         12     435
 
SacII CC_GC'GG

Cuts at:      0     386     447 
   Size:        386      61
 
StuI AGG'CCT

Cuts at:      0      46     265     447 
   Size:         46     219     182

  Fragments arranged by size: 

                 219     182      46
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      93     447 
   Size:         93     354
 

 Enzymes that do cut:

   AflIII     AlfI     ApoI     AvaI     BanI     BcgI Bme1580I    BmgBI
     BplI    BseRI    BseYI    BsiEI    BsmBI Bsp1286I    BspHI    BspMI
    BstYI     BtgI     EaeI    EcoRI     FalI    HaeII     MscI     MslI
   MspA1I     PasI    PvuII    SacII     StuI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI  BsiHKAI    BsiWI     BsmI    BspEI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI