Restriction map for
pFN21AE1411
(Linear) (Six-base) MAPSORT of: KIEE1411.seq Check: 7596 from: 1 to: 597
KIEE1411.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 286 465 597
Size: 286 179 132
Fragments arranged by size:
286 179 132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 277 597
Size: 64 213 320
Fragments arranged by size:
320 213 64
AfeI AGC'GCT
Cuts at: 0 508 597
Size: 508 89
AflIII A'CryG_T
Cuts at: 0 587 597
Size: 587 10
AlwNI CAG_nnn'CTG
Cuts at: 0 378 537 597
Size: 378 159 60
Fragments arranged by size:
378 159 60
ApaI G_GGCC'C
Cuts at: 0 196 385 597
Size: 196 189 212
Fragments arranged by size:
212 196 189
BamHI G'GATC_C
Cuts at: 0 314 597
Size: 314 283
BanII G_rGCy'C
Cuts at: 0 196 385 398 597
Size: 196 189 13 199
Fragments arranged by size:
199 196 189 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 274 308 597
Size: 274 34 289
Fragments arranged by size:
289 274 34
Bme1580I G_kGCm'C
Cuts at: 0 196 385 597
Size: 196 189 212
Fragments arranged by size:
212 196 189
BmrI ACTGGGnnnn_n'
Cuts at: 0 320 597
Size: 320 277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 597
Size: 61 536
BsaWI w'CCGG_w
Cuts at: 0 152 597
Size: 152 445
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 8 38 597
Size: 8 30 559
Fragments arranged by size:
559 30 8
BseYI C'CCAG_C
Cuts at: 0 45 378 597
Size: 45 333 219
Fragments arranged by size:
333 219 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 487 523 568 597
Size: 71 416 36 45 29
Fragments arranged by size:
416 71 45 36 29
Bsp1286I G_dGCh'C
Cuts at: 0 196 385 398 597
Size: 196 189 13 199
Fragments arranged by size:
199 196 189 13
BspEI T'CCGG_A
Cuts at: 0 152 597
Size: 152 445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 286 296 465 597
Size: 286 10 169 132
Fragments arranged by size:
286 169 132 10
BsrBI CCG'CTC
Cuts at: 0 29 597
Size: 29 568
BsrFI r'CCGG_y
Cuts at: 0 350 597
Size: 350 247
BstYI r'GATC_y
Cuts at: 0 314 597
Size: 314 283
BtgI C'CryG_G
Cuts at: 0 398 597
Size: 398 199
DraIII CAC_nnn'GTG
Cuts at: 0 465 597
Size: 465 132
EarI CTCTTCn'nnn_
Cuts at: 0 266 597
Size: 266 331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 64 277 597
Size: 61 3 213 320
Fragments arranged by size:
320 213 61 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 91 243 597
Size: 91 152 354
Fragments arranged by size:
354 152 91
EcoO109I rG'GnC_Cy
Cuts at: 0 192 193 382 597
Size: 192 1 189 215
Fragments arranged by size:
215 192 189 1
EcoRV GAT'ATC
Cuts at: 0 177 432 597
Size: 177 255 165
Fragments arranged by size:
255 177 165
HaeII r_GCGC'y
Cuts at: 0 510 597
Size: 510 87
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 597
Size: 572 25
MspA1I CmG'CkG
Cuts at: 0 366 440 597
Size: 366 74 157
Fragments arranged by size:
366 157 74
NcoI C'CATG_G
Cuts at: 0 398 597
Size: 398 199
NspI r_CATG'y
Cuts at: 0 591 597
Size: 591 6
PasI CC'CwG_GG
Cuts at: 0 239 597
Size: 239 358
PciI A'CATG_T
Cuts at: 0 587 597
Size: 587 10
PflMI CCAn_nnn'nTGG
Cuts at: 0 378 597
Size: 378 219
PspOMI G'GGCC_C
Cuts at: 0 192 381 597
Size: 192 189 216
Fragments arranged by size:
216 192 189
PstI C_TGCA'G
Cuts at: 0 297 597
Size: 297 300
PvuII CAG'CTG
Cuts at: 0 366 597
Size: 366 231
SbfI CC_TGCA'GG
Cuts at: 0 297 597
Size: 297 300
ScaI AGT'ACT
Cuts at: 0 23 597
Size: 23 574
SfcI C'TryA_G
Cuts at: 0 226 293 597
Size: 226 67 304
Fragments arranged by size:
304 226 67
StuI AGG'CCT
Cuts at: 0 149 487 597
Size: 149 338 110
Fragments arranged by size:
338 149 110
StyI C'CwwG_G
Cuts at: 0 398 597
Size: 398 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 37 298 597
Size: 37 261 299
Fragments arranged by size:
299 261 37
TatI w'GTAC_w
Cuts at: 0 21 597
Size: 21 576
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApaI BamHI BanII
BcgI Bme1580I BmrI BpmI BsaWI BsaXI BseYI BsgI
Bsp1286I BspEI BspMI BsrBI BsrFI BstYI BtgI DraIII
EarI Eco57MI EcoNI EcoO109I EcoRV HaeII MmeI MspA1I
NcoI NspI PasI PciI PflMI PspOMI PstI PvuII
SbfI ScaI SfcI StuI StyI TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BseRI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI
EcoRI FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI