Restriction map for
pFN21AE1409
(Linear) (Six-base) MAPSORT of: KIEE1409.seq Check: 9292 from: 1 to: 1404
KIEE1409.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1049 1404
Size: 1049 355
AfeI AGC'GCT
Cuts at: 0 161 1404
Size: 161 1243
AgeI A'CCGG_T
Cuts at: 0 437 1404
Size: 437 967
AhdI GACnn_n'nnGTC
Cuts at: 0 915 1404
Size: 915 489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 582 1404
Size: 582 822
AlwNI CAG_nnn'CTG
Cuts at: 0 1008 1027 1387 1404
Size: 1008 19 360 17
Fragments arranged by size:
1008 360 19 17
ApaI G_GGCC'C
Cuts at: 0 204 1404
Size: 204 1200
ApaLI G'TGCA_C
Cuts at: 0 441 1404
Size: 441 963
AscI GG'CGCG_CC
Cuts at: 0 213 1404
Size: 213 1191
AvaI C'yCGr_G
Cuts at: 0 58 197 203 1404
Size: 58 139 6 1201
Fragments arranged by size:
1201 139 58 6
BanI G'GyrC_C
Cuts at: 0 3 629 1404
Size: 3 626 775
Fragments arranged by size:
775 626 3
BanII G_rGCy'C
Cuts at: 0 118 204 1404
Size: 118 86 1200
Fragments arranged by size:
1200 118 86
BbeI G_GCGC'C
Cuts at: 0 7 1404
Size: 7 1397
BbvCI CC'TCA_GC
Cuts at: 0 872 1404
Size: 872 532
BclI T'GATC_A
Cuts at: 0 342 1404
Size: 342 1062
BlpI GC'TnA_GC
Cuts at: 0 318 1404
Size: 318 1086
Bme1580I G_kGCm'C
Cuts at: 0 198 204 445 634 1127 1404
Size: 198 6 241 189 493 277
Fragments arranged by size:
493 277 241 198 189 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 185 1404
Size: 185 1219
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 178 1404
Size: 178 1226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 690 1404
Size: 446 244 714
Fragments arranged by size:
714 446 244
Bpu10I CC'TnA_GC
Cuts at: 0 795 872 1404
Size: 795 77 532
Fragments arranged by size:
795 532 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 856 1040 1404
Size: 856 184 364
Fragments arranged by size:
856 364 184
BsaHI Gr'CG_yC
Cuts at: 0 4 1404
Size: 4 1400
BsaWI w'CCGG_w
Cuts at: 0 437 584 1404
Size: 437 147 820
Fragments arranged by size:
820 437 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 988 1018 1404
Size: 988 30 386
Fragments arranged by size:
988 386 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 170 1056 1059 1404
Size: 170 886 3 345
Fragments arranged by size:
886 345 170 3
BseYI C'CCAG_C
Cuts at: 0 100 1011 1404
Size: 100 911 393
Fragments arranged by size:
911 393 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 639 1404
Size: 522 117 765
Fragments arranged by size:
765 522 117
BsiHKAI G_wGCw'C
Cuts at: 0 445 1404
Size: 445 959
BsmBI CGTCTCn'nnnn_
Cuts at: 0 933 1404
Size: 933 471
Bsp1286I G_dGCh'C
Cuts at: 0 118 198 204 445 634 1127 1404
Size: 118 80 6 241 189 493 277
Fragments arranged by size:
493 277 241 189 118 80 6
BspEI T'CCGG_A
Cuts at: 0 584 1404
Size: 584 820
BspHI T'CATG_A
Cuts at: 0 339 1148 1404
Size: 339 809 256
Fragments arranged by size:
809 339 256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 294 1404
Size: 294 1110
BsrDI GCAATG_nn'
Cuts at: 0 370 711 1235 1404
Size: 370 341 524 169
Fragments arranged by size:
524 370 341 169
BsrFI r'CCGG_y
Cuts at: 0 231 437 957 1017 1404
Size: 231 206 520 60 387
Fragments arranged by size:
520 387 231 206 60
BsrGI T'GTAC_A
Cuts at: 0 499 981 1404
Size: 499 482 423
Fragments arranged by size:
499 482 423
BssHII G'CGCG_C
Cuts at: 0 213 1404
Size: 213 1191
BssSI C'ACGA_G
Cuts at: 0 140 1404
Size: 140 1264
BstAPI GCAn_nnn'nTGC
Cuts at: 0 629 1069 1404
Size: 629 440 335
Fragments arranged by size:
629 440 335
BstXI CCAn_nnnn'nTGG
Cuts at: 0 642 822 1404
Size: 642 180 582
Fragments arranged by size:
642 582 180
BstYI r'GATC_y
Cuts at: 0 403 1404
Size: 403 1001
BtgI C'CryG_G
Cuts at: 0 89 122 173 1404
Size: 89 33 51 1231
Fragments arranged by size:
1231 89 51 33
BtsI GCAGTG_nn'
Cuts at: 0 50 1404
Size: 50 1354
ClaI AT'CG_AT
Cuts at: 0 1181 1404
Size: 1181 223
EaeI y'GGCC_r
Cuts at: 0 92 1404
Size: 92 1312
EarI CTCTTCn'nnn_
Cuts at: 0 123 518 1301 1404
Size: 123 395 783 103
Fragments arranged by size:
783 395 123 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 690 1049 1404
Size: 446 244 359 355
Fragments arranged by size:
446 359 355 244
EcoNI CCTnn'n_nnAGG
Cuts at: 0 301 1296 1404
Size: 301 995 108
Fragments arranged by size:
995 301 108
EcoO109I rG'GnC_Cy
Cuts at: 0 223 294 810 862 1335 1404
Size: 223 71 516 52 473 69
Fragments arranged by size:
516 473 223 71 69 52
HaeII r_GCGC'y
Cuts at: 0 7 163 846 1404
Size: 7 156 683 558
Fragments arranged by size:
683 558 156 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 39 71 629 661 901 933 1404
Size: 39 32 558 32 240 32 471
Fragments arranged by size:
558 471 240 39 32 32 32
HincII GTy'rAC
Cuts at: 0 1248 1404
Size: 1248 156
KasI G'GCGC_C
Cuts at: 0 3 1404
Size: 3 1401
MfeI C'AATT_G
Cuts at: 0 350 1404
Size: 350 1054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1404
Size: 497 907
MslI CAynn'nnrTG
Cuts at: 0 29 363 564 1404
Size: 29 334 201 840
Fragments arranged by size:
840 334 201 29
MspA1I CmG'CkG
Cuts at: 0 91 124 322 1158 1404
Size: 91 33 198 836 246
Fragments arranged by size:
836 246 198 91 33
NaeI GCC'GGC
Cuts at: 0 233 959 1404
Size: 233 726 445
Fragments arranged by size:
726 445 233
NarI GG'CG_CC
Cuts at: 0 4 1404
Size: 4 1400
NcoI C'CATG_G
Cuts at: 0 173 1404
Size: 173 1231
NgoMIV G'CCGG_C
Cuts at: 0 231 957 1404
Size: 231 726 447
Fragments arranged by size:
726 447 231
NspI r_CATG'y
Cuts at: 0 563 1318 1404
Size: 563 755 86
Fragments arranged by size:
755 563 86
PasI CC'CwG_GG
Cuts at: 0 991 1210 1404
Size: 991 219 194
Fragments arranged by size:
991 219 194
PflMI CCAn_nnn'nTGG
Cuts at: 0 1066 1404
Size: 1066 338
PfoI T'CCnGG_A
Cuts at: 0 423 1404
Size: 423 981
PpuMI rG'GwC_Cy
Cuts at: 0 223 294 810 1335 1404
Size: 223 71 516 525 69
Fragments arranged by size:
525 516 223 71 69
PshAI GACnn'nnGTC
Cuts at: 0 973 1404
Size: 973 431
PspOMI G'GGCC_C
Cuts at: 0 200 1404
Size: 200 1204
PstI C_TGCA'G
Cuts at: 0 305 1404
Size: 305 1099
PvuII CAG'CTG
Cuts at: 0 322 1158 1404
Size: 322 836 246
Fragments arranged by size:
836 322 246
SacII CC_GC'GG
Cuts at: 0 92 125 1404
Size: 92 33 1279
Fragments arranged by size:
1279 92 33
SanDI GG'GwC_CC
Cuts at: 0 810 1404
Size: 810 594
SapI GCTCTTCn'nnn_
Cuts at: 0 123 1404
Size: 123 1281
SfcI C'TryA_G
Cuts at: 0 301 1404
Size: 301 1103
SfoI GGC'GCC
Cuts at: 0 5 1404
Size: 5 1399
SmaI CCC'GGG
Cuts at: 0 60 199 205 1404
Size: 60 139 6 1199
Fragments arranged by size:
1199 139 60 6
SrfI GCCC'GGGC
Cuts at: 0 199 205 1404
Size: 199 6 1199
Fragments arranged by size:
1199 199 6
StyI C'CwwG_G
Cuts at: 0 173 1292 1397 1404
Size: 173 1119 105 7
Fragments arranged by size:
1119 173 105 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 114 711 1404
Size: 114 597 693
Fragments arranged by size:
693 597 114
TatI w'GTAC_w
Cuts at: 0 21 499 981 1404
Size: 21 478 482 423
Fragments arranged by size:
482 478 423 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 802 817 834 849 988 1020 1404
Size: 802 15 17 15 139 32 384
Fragments arranged by size:
802 384 139 32 17 15 15
XmaI C'CCGG_G
Cuts at: 0 58 197 203 1404
Size: 58 139 6 1201
Fragments arranged by size:
1201 139 58 6
Enzymes that do cut:
AcuI AfeI AgeI AhdI AlfI AlwNI ApaI ApaLI
AscI AvaI BanI BanII BbeI BbvCI BclI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstXI BstYI BtgI BtsI ClaI EaeI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MfeI MmeI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacII
SanDI SapI SfcI SfoI SmaI SrfI StyI TaqII
TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AleI
AloI ApoI AseI AsiSI AvrII BaeI BamHI BbsI
BcgI BciVI BfrBI BglI BglII BmgBI BmtI BpuEI
BsaAI BsaBI BsiEI BsiWI BsmI BsrBI BstBI BstEII
BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MluI MscI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI
ZraI