(Linear) (Six-base) MAPSORT of: KIEE1408.seq  Check: 2890  from: 1  to: 441



KIEE1408.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:38  ..

AccI GT'mk_AC

Cuts at:      0     431     441 
   Size:        431      10
 
Acc65I G'GTAC_C

Cuts at:      0      93     441 
   Size:         93     348
 
AfeI AGC'GCT

Cuts at:      0     136     441 
   Size:        136     305
 
AflIII A'CryG_T

Cuts at:      0     102     441 
   Size:        102     339
 
BanI G'GyrC_C

Cuts at:      0      93     441 
   Size:         93     348
 
BanII G_rGCy'C

Cuts at:      0     350     441 
   Size:        350      91
 
Bme1580I G_kGCm'C

Cuts at:      0     118     441 
   Size:        118     323
 
BmgBI CAC'GTC

Cuts at:      0     288     441 
   Size:        288     153
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     334     441 
   Size:        332       2     107

  Fragments arranged by size: 

                 332     107       2
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     358     409     441 
   Size:        358      51      32

  Fragments arranged by size: 

                 358      51      32
 
BsiHKAI G_wGCw'C

Cuts at:      0     350     441 
   Size:        350      91
 
Bsp1286I G_dGCh'C

Cuts at:      0     118     350     441 
   Size:        118     232      91

  Fragments arranged by size: 

                 232     118      91
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     155     441 
   Size:        155     286
 
BtgI C'CryG_G

Cuts at:      0     219     441 
   Size:        219     222
 
EaeI y'GGCC_r

Cuts at:      0      15     124     164     441 
   Size:         15     109      40     277

  Fragments arranged by size: 

                 277     109      40      15
 
EcoICRI GAG'CTC

Cuts at:      0     348     441 
   Size:        348      93
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     334     441 
   Size:        332       2     107

  Fragments arranged by size: 

                 332     107       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0     224     441 
   Size:        224     217
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     387     441 
   Size:        387      54
 
HaeII r_GCGC'y

Cuts at:      0     138     441 
   Size:        138     303
 
KpnI G_GTAC'C

Cuts at:      0      97     441 
   Size:         97     344
 
MscI TGG'CCA

Cuts at:      0      17     441 
   Size:         17     424
 
MslI CAynn'nnrTG

Cuts at:      0     218     290     326     441 
   Size:        218      72      36     115

  Fragments arranged by size: 

                 218     115      72      36
 
MspA1I CmG'CkG

Cuts at:      0     120     441 
   Size:        120     321
 
NcoI C'CATG_G

Cuts at:      0     219     441 
   Size:        219     222
 
NspI r_CATG'y

Cuts at:      0     106     441 
   Size:        106     335
 
PciI A'CATG_T

Cuts at:      0     102     441 
   Size:        102     339
 
PpuMI rG'GwC_Cy

Cuts at:      0     224     441 
   Size:        224     217
 
PshAI GACnn'nnGTC

Cuts at:      0     149     441 
   Size:        149     292
 
SacI G_AGCT'C

Cuts at:      0     350     441 
   Size:        350      91
 
SfcI C'TryA_G

Cuts at:      0      38     441 
   Size:         38     403
 
StyI C'CwwG_G

Cuts at:      0     219     441 
   Size:        219     222
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     257     289     441 
   Size:        257      32     152

  Fragments arranged by size: 

                 257     152      32
 

 Enzymes that do cut:

     AccI   Acc65I     AfeI   AflIII     BanI    BanII Bme1580I    BmgBI
     BpmI    BseRI  BsiHKAI Bsp1286I    BspMI     BtgI     EaeI  EcoICRI
  Eco57MI EcoO109I     FalI    HaeII     KpnI     MscI     MslI   MspA1I
     NcoI     NspI     PciI    PpuMI    PshAI     SacI     SfcI     StyI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI