Restriction map for
pFN21AE1407
(Linear) (Six-base) MAPSORT of: KIEE1407.seq Check: 4377 from: 1 to: 975
KIEE1407.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 135 975
Size: 135 840
Acc65I G'GTAC_C
Cuts at: 0 60 975
Size: 60 915
AlwNI CAG_nnn'CTG
Cuts at: 0 795 975
Size: 795 180
ApaI G_GGCC'C
Cuts at: 0 968 975
Size: 968 7
ApoI r'AATT_y
Cuts at: 0 87 274 777 975
Size: 87 187 503 198
Fragments arranged by size:
503 198 187 87
AvaI C'yCGr_G
Cuts at: 0 943 975
Size: 943 32
AvrII C'CTAG_G
Cuts at: 0 772 975
Size: 772 203
BanI G'GyrC_C
Cuts at: 0 60 975
Size: 60 915
BanII G_rGCy'C
Cuts at: 0 968 975
Size: 968 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 684 757 905 975
Size: 684 73 148 70
Fragments arranged by size:
684 148 73 70
BfrBI ATG'CAT
Cuts at: 0 197 975
Size: 197 778
Bme1580I G_kGCm'C
Cuts at: 0 968 975
Size: 968 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 306 507 975
Size: 306 201 468
Fragments arranged by size:
468 306 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 975
Size: 562 413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 279 975
Size: 213 66 696
Fragments arranged by size:
696 213 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 751 781 975
Size: 751 30 194
Fragments arranged by size:
751 194 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 703 975
Size: 703 272
BseYI C'CCAG_C
Cuts at: 0 24 601 975
Size: 24 577 374
Fragments arranged by size:
577 374 24
BsmI GAATG_Cn'
Cuts at: 0 288 356 975
Size: 288 68 619
Fragments arranged by size:
619 288 68
Bsp1286I G_dGCh'C
Cuts at: 0 968 975
Size: 968 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 135 975
Size: 135 840
BsrBI CCG'CTC
Cuts at: 0 911 975
Size: 911 64
BsrDI GCAATG_nn'
Cuts at: 0 890 975
Size: 890 85
BstEII G'GTnAC_C
Cuts at: 0 247 975
Size: 247 728
BtgI C'CryG_G
Cuts at: 0 547 975
Size: 547 428
DrdI GACnn_nn'nnGTC
Cuts at: 0 526 649 975
Size: 526 123 326
Fragments arranged by size:
526 326 123
EaeI y'GGCC_r
Cuts at: 0 668 975
Size: 668 307
EarI CTCTTCn'nnn_
Cuts at: 0 788 975
Size: 788 187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 975
Size: 562 413
EcoO109I rG'GnC_Cy
Cuts at: 0 528 947 964 965 975
Size: 528 419 17 1 10
Fragments arranged by size:
528 419 17 10 1
EcoRI G'AATT_C
Cuts at: 0 87 777 975
Size: 87 690 198
Fragments arranged by size:
690 198 87
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 780 975
Size: 780 195
KpnI G_GTAC'C
Cuts at: 0 64 975
Size: 64 911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 975
Size: 836 139
MslI CAynn'nnrTG
Cuts at: 0 679 975
Size: 679 296
MspA1I CmG'CkG
Cuts at: 0 935 975
Size: 935 40
NcoI C'CATG_G
Cuts at: 0 547 975
Size: 547 428
NdeI CA'TA_TG
Cuts at: 0 383 975
Size: 383 592
NsiI A_TGCA'T
Cuts at: 0 199 975
Size: 199 776
PfoI T'CCnGG_A
Cuts at: 0 658 975
Size: 658 317
PpuMI rG'GwC_Cy
Cuts at: 0 528 947 975
Size: 528 419 28
Fragments arranged by size:
528 419 28
PsiI TTA'TAA
Cuts at: 0 461 975
Size: 461 514
PspOMI G'GGCC_C
Cuts at: 0 964 975
Size: 964 11
PstI C_TGCA'G
Cuts at: 0 940 975
Size: 940 35
PvuII CAG'CTG
Cuts at: 0 935 975
Size: 935 40
SanDI GG'GwC_CC
Cuts at: 0 947 975
Size: 947 28
ScaI AGT'ACT
Cuts at: 0 365 975
Size: 365 610
SfcI C'TryA_G
Cuts at: 0 112 936 975
Size: 112 824 39
Fragments arranged by size:
824 112 39
SmlI C'TyrA_G
Cuts at: 0 228 258 975
Size: 228 30 717
Fragments arranged by size:
717 228 30
StyI C'CwwG_G
Cuts at: 0 12 547 772 975
Size: 12 535 225 203
Fragments arranged by size:
535 225 203 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 16 678 975
Size: 16 662 297
Fragments arranged by size:
662 297 16
TatI w'GTAC_w
Cuts at: 0 363 975
Size: 363 612
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 300 332 975
Size: 300 32 643
Fragments arranged by size:
643 300 32
XmnI GAAnn'nnTTC
Cuts at: 0 87 497 975
Size: 87 410 478
Fragments arranged by size:
478 410 87
Enzymes that do cut:
AarI Acc65I AlwNI ApaI ApoI AvaI AvrII BanI
BanII BbsI BfrBI Bme1580I BmrI BpmI BpuEI BsaXI
BseRI BseYI BsmI Bsp1286I BspMI BsrBI BsrDI BstEII
BtgI DrdI EaeI EarI Eco57MI EcoO109I EcoRI FalI
KpnI MmeI MslI MspA1I NcoI NdeI NsiI PfoI
PpuMI PsiI PspOMI PstI PvuII SanDI ScaI SfcI
SmlI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII EagI EciI EcoICRI EcoNI EcoRV FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
MfeI MluI MscI NaeI NarI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI