Restriction map for
pFN21AE1406
(Linear) (Six-base) MAPSORT of: KIEE1406.seq Check: 3013 from: 1 to: 1716
KIEE1406.orf
With 182 enzymes: SgfI *
July 3, 2012 16:51 ..
AatII G_ACGT'C
Cuts at: 0 104 1716
Size: 104 1612
AccI GT'mk_AC
Cuts at: 0 104 497 518 1716
Size: 104 393 21 1198
Fragments arranged by size:
1198 393 104 21
Acc65I G'GTAC_C
Cuts at: 0 1515 1716
Size: 1515 201
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 1535 1599 1716
Size: 704 831 64 117
Fragments arranged by size:
831 704 117 64
AflII C'TTAA_G
Cuts at: 0 562 1716
Size: 562 1154
AflIII A'CryG_T
Cuts at: 0 218 1716
Size: 218 1498
ApaI G_GGCC'C
Cuts at: 0 815 1716
Size: 815 901
AvaI C'yCGr_G
Cuts at: 0 658 1054 1716
Size: 658 396 662
Fragments arranged by size:
662 658 396
BamHI G'GATC_C
Cuts at: 0 632 1716
Size: 632 1084
BanI G'GyrC_C
Cuts at: 0 859 1515 1627 1677 1716
Size: 859 656 112 50 39
Fragments arranged by size:
859 656 112 50 39
BanII G_rGCy'C
Cuts at: 0 815 833 925 1213 1716
Size: 815 18 92 288 503
Fragments arranged by size:
815 503 288 92 18
BbeI G_GCGC'C
Cuts at: 0 1681 1716
Size: 1681 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 172 1258 1370 1716
Size: 172 1086 112 346
Fragments arranged by size:
1086 346 172 112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 649 683 1716
Size: 649 34 1033
Fragments arranged by size:
1033 649 34
BclI T'GATC_A
Cuts at: 0 308 1716
Size: 308 1408
BglI GCCn_nnn'nGGC
Cuts at: 0 1021 1716
Size: 1021 695
BglII A'GATC_T
Cuts at: 0 1169 1716
Size: 1169 547
Bme1580I G_kGCm'C
Cuts at: 0 815 1632 1716
Size: 815 817 84
Fragments arranged by size:
817 815 84
BmgBI CAC'GTC
Cuts at: 0 1224 1716
Size: 1224 492
BmrI ACTGGGnnnn_n'
Cuts at: 0 891 1716
Size: 891 825
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 475 658 712 891 1716
Size: 130 345 183 54 179 825
Fragments arranged by size:
825 345 183 179 130 54
Bpu10I CC'TnA_GC
Cuts at: 0 617 673 919 1716
Size: 617 56 246 797
Fragments arranged by size:
797 617 246 56
BsaAI yAC'GTr
Cuts at: 0 221 414 1716
Size: 221 193 1302
Fragments arranged by size:
1302 221 193
BsaBI GATnn'nnATC
Cuts at: 0 646 1716
Size: 646 1070
BsaHI Gr'CG_yC
Cuts at: 0 101 479 1678 1716
Size: 101 378 1199 38
Fragments arranged by size:
1199 378 101 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 897 927 937 967 1417 1447 1530 1560
Size: 897 30 10 30 450 30 83 30
Cuts at: 1560 1716
Size: 156
Fragments arranged by size:
897 450 156 83 30 30 30 30
10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 463 1090 1716
Size: 40 423 627 626
Fragments arranged by size:
627 626 423 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 797 1716
Size: 487 310 919
Fragments arranged by size:
919 487 310
BsiEI CG_ry'CG
Cuts at: 0 1091 1716
Size: 1091 625
BsiHKAI G_wGCw'C
Cuts at: 0 1430 1716
Size: 1430 286
Bsp1286I G_dGCh'C
Cuts at: 0 815 833 925 1213 1430 1632 1716
Size: 815 18 92 288 217 202 84
Fragments arranged by size:
815 288 217 202 92 84 18
BspHI T'CATG_A
Cuts at: 0 764 1716
Size: 764 952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 233 969 1716
Size: 233 736 747
Fragments arranged by size:
747 736 233
BsrBI CCG'CTC
Cuts at: 0 1080 1546 1694 1716
Size: 1080 466 148 22
Fragments arranged by size:
1080 466 148 22
BsrDI GCAATG_nn'
Cuts at: 0 722 833 1716
Size: 722 111 883
Fragments arranged by size:
883 722 111
BsrFI r'CCGG_y
Cuts at: 0 1660 1716
Size: 1660 56
BsrGI T'GTAC_A
Cuts at: 0 749 1716
Size: 749 967
BssSI C'ACGA_G
Cuts at: 0 36 1716
Size: 36 1680
BstYI r'GATC_y
Cuts at: 0 632 1169 1716
Size: 632 537 547
Fragments arranged by size:
632 547 537
BtgI C'CryG_G
Cuts at: 0 1205 1317 1408 1716
Size: 1205 112 91 308
Fragments arranged by size:
1205 308 112 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 774 1716
Size: 774 942
BtsI GCAGTG_nn'
Cuts at: 0 1010 1716
Size: 1010 706
DrdI GACnn_nn'nnGTC
Cuts at: 0 478 979 1716
Size: 478 501 737
Fragments arranged by size:
737 501 478
EaeI y'GGCC_r
Cuts at: 0 797 993 1662 1689 1716
Size: 797 196 669 27 27
Fragments arranged by size:
797 669 196 27 27
EarI CTCTTCn'nnn_
Cuts at: 0 13 680 1716
Size: 13 667 1036
Fragments arranged by size:
1036 667 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 201 1212 1716
Size: 201 1011 504
Fragments arranged by size:
1011 504 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 475 658 704 712 891 1535 1599
Size: 130 345 183 46 8 179 644 64
Cuts at: 1599 1716
Size: 117
Fragments arranged by size:
644 345 183 179 130 117 64 46
8
EcoO109I rG'GnC_Cy
Cuts at: 0 567 655 811 812 1236 1716
Size: 567 88 156 1 424 480
Fragments arranged by size:
567 480 424 156 88 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1056 1374 1716
Size: 1056 318 342
Fragments arranged by size:
1056 342 318
HaeII r_GCGC'y
Cuts at: 0 1681 1716
Size: 1681 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 38 56 70 88 102 373 405 566
Size: 38 18 14 18 14 271 32 161
Cuts at: 566 598 897 929 1716
Size: 32 299 32 787
Fragments arranged by size:
787 299 271 161 38 32 32 32
18 18 14 14
HincII GTy'rAC
Cuts at: 0 216 1065 1383 1716
Size: 216 849 318 333
Fragments arranged by size:
849 333 318 216
KasI G'GCGC_C
Cuts at: 0 1677 1716
Size: 1677 39
KpnI G_GTAC'C
Cuts at: 0 1519 1716
Size: 1519 197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 1634 1716
Size: 554 1080 82
Fragments arranged by size:
1080 554 82
MscI TGG'CCA
Cuts at: 0 799 1716
Size: 799 917
MspA1I CmG'CkG
Cuts at: 0 379 992 1410 1716
Size: 379 613 418 306
Fragments arranged by size:
613 418 379 306
NarI GG'CG_CC
Cuts at: 0 1678 1716
Size: 1678 38
NspI r_CATG'y
Cuts at: 0 1494 1716
Size: 1494 222
PasI CC'CwG_GG
Cuts at: 0 240 1716
Size: 240 1476
PfoI T'CCnGG_A
Cuts at: 0 198 635 1421 1716
Size: 198 437 786 295
Fragments arranged by size:
786 437 295 198
PmlI CAC'GTG
Cuts at: 0 414 1716
Size: 414 1302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 1716
Size: 691 32 993
Fragments arranged by size:
993 691 32
PpuMI rG'GwC_Cy
Cuts at: 0 655 1236 1716
Size: 655 581 480
Fragments arranged by size:
655 581 480
PshAI GACnn'nnGTC
Cuts at: 0 1090 1716
Size: 1090 626
PspOMI G'GGCC_C
Cuts at: 0 811 1716
Size: 811 905
PstI C_TGCA'G
Cuts at: 0 230 1716
Size: 230 1486
PvuII CAG'CTG
Cuts at: 0 379 1716
Size: 379 1337
SacII CC_GC'GG
Cuts at: 0 1411 1716
Size: 1411 305
SanDI GG'GwC_CC
Cuts at: 0 655 1236 1716
Size: 655 581 480
Fragments arranged by size:
655 581 480
SapI GCTCTTCn'nnn_
Cuts at: 0 13 680 1716
Size: 13 667 1036
Fragments arranged by size:
1036 667 13
SfcI C'TryA_G
Cuts at: 0 226 1005 1716
Size: 226 779 711
Fragments arranged by size:
779 711 226
SfoI GGC'GCC
Cuts at: 0 1679 1716
Size: 1679 37
SmlI C'TyrA_G
Cuts at: 0 562 1716
Size: 562 1154
SpeI A'CTAG_T
Cuts at: 0 1329 1716
Size: 1329 387
StyI C'CwwG_G
Cuts at: 0 270 758 887 1186 1716
Size: 270 488 129 299 530
Fragments arranged by size:
530 488 299 270 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 59 868 877 1542 1602 1716
Size: 59 809 9 665 60 114
Fragments arranged by size:
809 665 114 60 59 9
TatI w'GTAC_w
Cuts at: 0 749 1289 1716
Size: 749 540 427
Fragments arranged by size:
749 540 427
Tth111I GACn'n_nGTC
Cuts at: 0 1225 1716
Size: 1225 491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 186 285 598 1716
Size: 186 99 313 1118
Fragments arranged by size:
1118 313 186 99
XmnI GAAnn'nnTTC
Cuts at: 0 1417 1716
Size: 1417 299
ZraI GAC'GTC
Cuts at: 0 102 1716
Size: 102 1614
Enzymes that do cut:
AatII AccI Acc65I AcuI AflII AflIII ApaI AvaI
BamHI BanI BanII BbeI BbsI BcgI BclI BglI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaBI
BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstYI BtgI
BtgZI BtsI DrdI EaeI EarI EciI Eco57MI EcoO109I
FalI HaeII Hin4I HincII KasI KpnI MmeI MscI
MspA1I NarI NspI PasI PfoI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuII SacII SanDI SapI SfcI
SfoI SmlI SpeI StyI TaqII TatI Tth111I XcmI
XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AgeI AhdI AleI AlfI AloI
AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbvCI BciVI BfrBI BlpI BmtI BplI BpuEI BsaI
BsaWI BseYI BsiWI BsmI BsmBI BspEI BssHII BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI
DraIII EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI MfeI MluI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PmeI PsiI PspXI PsrI PvuI RsrII SacI
SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI StuI SwaI TstI XbaI
XhoI XmaI