(Linear) (Six-base) MAPSORT of: KIEE1403.seq  Check: 82  from: 1  to: 1122



KIEE1403.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:38  ..

AatII G_ACGT'C

Cuts at:      0     248    1122 
   Size:        248     874
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1003    1122 
   Size:       1003     119
 
AflIII A'CryG_T

Cuts at:      0     701    1122 
   Size:        701     421
 
AgeI A'CCGG_T

Cuts at:      0     322     425    1122 
   Size:        322     103     697

  Fragments arranged by size: 

                 697     322     103
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1104    1122 
   Size:       1104      18
 
ApoI r'AATT_y

Cuts at:      0      18     735     837    1122 
   Size:         18     717     102     285

  Fragments arranged by size: 

                 717     285     102      18
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     583     616    1122 
   Size:        583      33     506

  Fragments arranged by size: 

                 583     506      33
 
BanII G_rGCy'C

Cuts at:      0     620    1122 
   Size:        620     502
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     543    1122 
   Size:        543     579
 
BclI T'GATC_A

Cuts at:      0     203    1122 
   Size:        203     919
 
BglII A'GATC_T

Cuts at:      0     986    1122 
   Size:        986     136
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     117     519    1122 
   Size:         24      93     402     603

  Fragments arranged by size: 

                 603     402      93      24
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     670    1122 
   Size:        670     452
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1078    1122 
   Size:       1078      44
 
BsaAI yAC'GTr

Cuts at:      0     350    1122 
   Size:        350     772
 
BsaHI Gr'CG_yC

Cuts at:      0     245    1122 
   Size:        245     877
 
BsaWI w'CCGG_w

Cuts at:      0     322     425    1122 
   Size:        322     103     697

  Fragments arranged by size: 

                 697     322     103
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     904    1122 
   Size:        904     218
 
BsiHKAI G_wGCw'C

Cuts at:      0     109     944    1122 
   Size:        109     835     178

  Fragments arranged by size: 

                 835     178     109
 
Bsp1286I G_dGCh'C

Cuts at:      0     109     620     944    1122 
   Size:        109     511     324     178

  Fragments arranged by size: 

                 511     324     178     109
 
BspHI T'CATG_A

Cuts at:      0     686    1122 
   Size:        686     436
 
BsrBI CCG'CTC

Cuts at:      0       5    1122 
   Size:          5    1117
 
BsrDI GCAATG_nn'

Cuts at:      0     266     348    1122 
   Size:        266      82     774

  Fragments arranged by size: 

                 774     266      82
 
BsrFI r'CCGG_y

Cuts at:      0     322     425    1122 
   Size:        322     103     697

  Fragments arranged by size: 

                 697     322     103
 
BsrGI T'GTAC_A

Cuts at:      0     441    1122 
   Size:        441     681
 
BstYI r'GATC_y

Cuts at:      0     986    1122 
   Size:        986     136
 
Bsu36I CC'TnA_GG

Cuts at:      0    1029    1122 
   Size:       1029      93
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      41     417     596    1122 
   Size:         41     376     179     526

  Fragments arranged by size: 

                 526     376     179      41
 
BtsI GCAGTG_nn'

Cuts at:      0     314     921    1122 
   Size:        314     607     201

  Fragments arranged by size: 

                 607     314     201
 
EaeI y'GGCC_r

Cuts at:      0     263    1122 
   Size:        263     859
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     117     519    1003    1122 
   Size:         24      93     402     484     119

  Fragments arranged by size: 

                 484     402     119      93      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     996    1122 
   Size:        996     126
 
EcoRI G'AATT_C

Cuts at:      0     735    1122 
   Size:        735     387
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1090    1122 
   Size:       1090      32
 
MscI TGG'CCA

Cuts at:      0     265    1122 
   Size:        265     857
 
MslI CAynn'nnrTG

Cuts at:      0     418     772    1122 
   Size:        418     354     350

  Fragments arranged by size: 

                 418     354     350
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     626    1122 
   Size:        626     496
 
PmlI CAC'GTG

Cuts at:      0     350    1122 
   Size:        350     772
 
PpuMI rG'GwC_Cy

Cuts at:      0     996    1122 
   Size:        996     126
 
PshAI GACnn'nnGTC

Cuts at:      0     244    1122 
   Size:        244     878
 
PsiI TTA'TAA

Cuts at:      0     221    1122 
   Size:        221     901
 
SgrAI Cr'CCGG_yG

Cuts at:      0     322    1122 
   Size:        322     800
 
SmlI C'TyrA_G

Cuts at:      0     649    1122 
   Size:        649     473
 
SspI AAT'ATT

Cuts at:      0     192    1122 
   Size:        192     930
 
StyI C'CwwG_G

Cuts at:      0     573     719    1122 
   Size:        573     146     403

  Fragments arranged by size: 

                 573     403     146
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     586    1122 
   Size:        586     536
 
TatI w'GTAC_w

Cuts at:      0     441    1019    1122 
   Size:        441     578     103

  Fragments arranged by size: 

                 578     441     103
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     988    1020    1122 
   Size:        988      32     102

  Fragments arranged by size: 

                 988     102      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     453    1122 
   Size:        453     669
 
ZraI GAC'GTC

Cuts at:      0     246    1122 
   Size:        246     876
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AgeI     AhdI     ApoI     BaeI    BanII
     BbsI     BclI    BglII     BpmI    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI    BstYI   Bsu36I    BtgZI     BtsI     EaeI  Eco57MI EcoO109I
    EcoRI    Hin4I     MscI     MslI    PflMI     PmlI    PpuMI    PshAI
     PsiI    SgrAI     SmlI     SspI     StyI    TaqII     TatI     TstI
  Tth111I     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BsaBI    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspMI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PpiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     XbaI     XcmI     XhoI
     XmaI     XmnI