(Linear) (Six-base) MAPSORT of: KIEE1400.seq  Check: 2130  from: 1  to: 309



KIEE1400.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:38  ..

AclI AA'CG_TT

Cuts at:      0     194     309 
   Size:        194     115
 
AflII C'TTAA_G

Cuts at:      0     175     309 
   Size:        175     134
 
AvaI C'yCGr_G

Cuts at:      0     164     309 
   Size:        164     145
 
BlpI GC'TnA_GC

Cuts at:      0      47     309 
   Size:         47     262
 
BsaHI Gr'CG_yC

Cuts at:      0     128     206     309 
   Size:        128      78     103

  Fragments arranged by size: 

                 128     103      78
 
BsaWI w'CCGG_w

Cuts at:      0     142     309 
   Size:        142     167
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     309 
   Size:        172     137
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     227     309 
   Size:         68     159      82

  Fragments arranged by size: 

                 159      82      68
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     197     309 
   Size:        197     112
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     227     309 
   Size:         68     159      82

  Fragments arranged by size: 

                 159      82      68
 
BsrFI r'CCGG_y

Cuts at:      0      96     309 
   Size:         96     213
 
BtgI C'CryG_G

Cuts at:      0     251     309 
   Size:        251      58
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      35     309 
   Size:         35     274
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     201     309 
   Size:        201     108
 
HaeII r_GCGC'y

Cuts at:      0      54     309 
   Size:         54     255
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     194     226     309 
   Size:        194      32      83

  Fragments arranged by size: 

                 194      83      32
 
MslI CAynn'nnrTG

Cuts at:      0     250     309 
   Size:        250      59
 
NcoI C'CATG_G

Cuts at:      0     251     309 
   Size:        251      58
 
PfoI T'CCnGG_A

Cuts at:      0      68     309 
   Size:         68     241
 
PspXI vC'TCGA_Gb

Cuts at:      0     164     309 
   Size:        164     145
 
SmlI C'TyrA_G

Cuts at:      0     164     175     309 
   Size:        164      11     134

  Fragments arranged by size: 

                 164     134      11
 
StyI C'CwwG_G

Cuts at:      0     251     309 
   Size:        251      58
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27      59     309 
   Size:         27      32     250

  Fragments arranged by size: 

                 250      32      27
 
Tth111I GACn'n_nGTC

Cuts at:      0     208     309 
   Size:        208     101
 
XhoI C'TCGA_G

Cuts at:      0     164     309 
   Size:        164     145
 

 Enzymes that do cut:

     AclI    AflII     AvaI     BlpI    BsaHI    BsaWI    BseRI  BsiHKAI
    BsmBI Bsp1286I    BsrFI     BtgI     EciI     FalI    HaeII    Hin4I
     MslI     NcoI     PfoI    PspXI     SmlI     StyI     TstI  Tth111I
     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     XbaI     XcmI
     XmaI     XmnI     ZraI