(Linear) (Six-base) MAPSORT of: KIEE1392.seq  Check: 6162  from: 1  to: 279



KIEE1392.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:37  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     279 
   Size:        257      22
 
BamHI G'GATC_C

Cuts at:      0     138     279 
   Size:        138     141
 
BanI G'GyrC_C

Cuts at:      0     102     279 
   Size:        102     177
 
Bme1580I G_kGCm'C

Cuts at:      0      83     279 
   Size:         83     196
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     279 
   Size:        277       2
 
Bpu10I CC'TnA_GC

Cuts at:      0     199     279 
   Size:        199      80
 
BseYI C'CCAG_C

Cuts at:      0     219     279 
   Size:        219      60
 
BsiHKAI G_wGCw'C

Cuts at:      0     185     279 
   Size:        185      94
 
Bsp1286I G_dGCh'C

Cuts at:      0      83     185     279 
   Size:         83     102      94

  Fragments arranged by size: 

                 102      94      83
 
BstEII G'GTnAC_C

Cuts at:      0      27     279 
   Size:         27     252
 
BstYI r'GATC_y

Cuts at:      0     138     279 
   Size:        138     141
 
DraIII CAC_nnn'GTG

Cuts at:      0     189     276     279 
   Size:        189      87       3

  Fragments arranged by size: 

                 189      87       3
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     277     279 
   Size:        257      20       2

  Fragments arranged by size: 

                 257      20       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     190     279 
   Size:        190      89
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     100     279 
   Size:        100     179
 
HaeII r_GCGC'y

Cuts at:      0     265     279 
   Size:        265      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     250     279 
   Size:        250      29
 
HincII GTy'rAC

Cuts at:      0      93     279 
   Size:         93     186
 
HindIII A'AGCT_T

Cuts at:      0     148     279 
   Size:        148     131
 

 Enzymes that do cut:

     AcuI    BamHI     BanI Bme1580I     BpmI   Bpu10I    BseYI  BsiHKAI
 Bsp1286I   BstEII    BstYI   DraIII  Eco57MI    EcoNI     FalI    HaeII
    Hin4I   HincII  HindIII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI