Restriction map for
pFN21AE1379
(Linear) (Six-base) MAPSORT of: KIEE1379.seq Check: 6260 from: 1 to: 759
KIEE1379.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 759
Size: 397 362
AfeI AGC'GCT
Cuts at: 0 397 432 759
Size: 397 35 327
Fragments arranged by size:
397 327 35
AflIII A'CryG_T
Cuts at: 0 28 759
Size: 28 731
AleI CACnn'nnGTG
Cuts at: 0 464 759
Size: 464 295
AlwNI CAG_nnn'CTG
Cuts at: 0 351 551 759
Size: 351 200 208
Fragments arranged by size:
351 208 200
BamHI G'GATC_C
Cuts at: 0 506 759
Size: 506 253
BanI G'GyrC_C
Cuts at: 0 34 163 759
Size: 34 129 596
Fragments arranged by size:
596 129 34
BanII G_rGCy'C
Cuts at: 0 365 759
Size: 365 394
BbsI GAAGACnn'nnnn_
Cuts at: 0 28 759
Size: 28 731
BciVI GTATCCnnnnn_n'
Cuts at: 0 334 759
Size: 334 425
Bme1580I G_kGCm'C
Cuts at: 0 205 759
Size: 205 554
BmrI ACTGGGnnnn_n'
Cuts at: 0 230 759
Size: 230 529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 759
Size: 328 431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 115 349 727 759
Size: 31 84 234 378 32
Fragments arranged by size:
378 234 84 32 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 578 631 759
Size: 578 53 128
Fragments arranged by size:
578 128 53
BsaAI yAC'GTr
Cuts at: 0 722 759
Size: 722 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 577 607 759
Size: 577 30 152
Fragments arranged by size:
577 152 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 759
Size: 280 479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 759
Size: 718 41
BsiHKAI G_wGCw'C
Cuts at: 0 365 656 755 759
Size: 365 291 99 4
Fragments arranged by size:
365 291 99 4
Bsp1286I G_dGCh'C
Cuts at: 0 205 365 656 755 759
Size: 205 160 291 99 4
Fragments arranged by size:
291 205 160 99 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 272 690 759
Size: 62 210 418 69
Fragments arranged by size:
418 210 69 62
BsrBI CCG'CTC
Cuts at: 0 512 759
Size: 512 247
BsrDI GCAATG_nn'
Cuts at: 0 686 759
Size: 686 73
BsrFI r'CCGG_y
Cuts at: 0 523 759
Size: 523 236
BsrGI T'GTAC_A
Cuts at: 0 731 759
Size: 731 28
BstAPI GCAn_nnn'nTGC
Cuts at: 0 41 759
Size: 41 718
BstXI CCAn_nnnn'nTGG
Cuts at: 0 435 466 759
Size: 435 31 293
Fragments arranged by size:
435 293 31
BstYI r'GATC_y
Cuts at: 0 506 759
Size: 506 253
Bsu36I CC'TnA_GG
Cuts at: 0 159 658 759
Size: 159 499 101
Fragments arranged by size:
499 159 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 300 759
Size: 300 459
BtsI GCAGTG_nn'
Cuts at: 0 113 459 759
Size: 113 346 300
Fragments arranged by size:
346 300 113
EaeI y'GGCC_r
Cuts at: 0 173 528 759
Size: 173 355 231
Fragments arranged by size:
355 231 173
EcoICRI GAG'CTC
Cuts at: 0 363 759
Size: 363 396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 397 759
Size: 328 69 362
Fragments arranged by size:
362 328 69
HaeII r_GCGC'y
Cuts at: 0 399 434 759
Size: 399 35 325
Fragments arranged by size:
399 325 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 577 609 759
Size: 577 32 150
Fragments arranged by size:
577 150 32
MfeI C'AATT_G
Cuts at: 0 69 759
Size: 69 690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 614 620 759
Size: 238 376 6 139
Fragments arranged by size:
376 238 139 6
MscI TGG'CCA
Cuts at: 0 175 759
Size: 175 584
MslI CAynn'nnrTG
Cuts at: 0 106 190 464 759
Size: 106 84 274 295
Fragments arranged by size:
295 274 106 84
MspA1I CmG'CkG
Cuts at: 0 351 444 548 759
Size: 351 93 104 211
Fragments arranged by size:
351 211 104 93
NspI r_CATG'y
Cuts at: 0 32 759
Size: 32 727
PciI A'CATG_T
Cuts at: 0 28 759
Size: 28 731
PfoI T'CCnGG_A
Cuts at: 0 647 759
Size: 647 112
PmlI CAC'GTG
Cuts at: 0 722 759
Size: 722 37
PshAI GACnn'nnGTC
Cuts at: 0 223 759
Size: 223 536
PstI C_TGCA'G
Cuts at: 0 449 759
Size: 449 310
PvuII CAG'CTG
Cuts at: 0 351 444 548 759
Size: 351 93 104 211
Fragments arranged by size:
351 211 104 93
SacI G_AGCT'C
Cuts at: 0 365 759
Size: 365 394
SfcI C'TryA_G
Cuts at: 0 445 759
Size: 445 314
SmlI C'TyrA_G
Cuts at: 0 46 130 364 742 759
Size: 46 84 234 378 17
Fragments arranged by size:
378 234 84 46 17
TatI w'GTAC_w
Cuts at: 0 275 731 759
Size: 275 456 28
Fragments arranged by size:
456 275 28
Enzymes that do cut:
AcuI AfeI AflIII AleI AlwNI BamHI BanI BanII
BbsI BciVI Bme1580I BmrI BpmI BpuEI BsaI BsaAI
BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgZI BtsI
EaeI EcoICRI Eco57MI HaeII Hin4I MfeI MmeI MscI
MslI MspA1I NspI PciI PfoI PmlI PshAI PstI
PvuII SacI SfcI SmlI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaBI
BsaHI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BssHII BssSI BstBI BstEII BstZ17I BtgI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PpiI PpuMI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI