Restriction map for
pFN21AE1378
(Linear) (Six-base) MAPSORT of: KIEE1378.seq Check: 5515 from: 1 to: 747
KIEE1378.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AflIII A'CryG_T
Cuts at: 0 60 747
Size: 60 687
AlwNI CAG_nnn'CTG
Cuts at: 0 232 747
Size: 232 515
ApaI G_GGCC'C
Cuts at: 0 27 747
Size: 27 720
ApaLI G'TGCA_C
Cuts at: 0 118 747
Size: 118 629
ApoI r'AATT_y
Cuts at: 0 69 747
Size: 69 678
AvaI C'yCGr_G
Cuts at: 0 26 747
Size: 26 721
BanII G_rGCy'C
Cuts at: 0 27 623 747
Size: 27 596 124
Fragments arranged by size:
596 124 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 63 747
Size: 63 684
BglI GCCn_nnn'nGGC
Cuts at: 0 31 747
Size: 31 716
Bme1580I G_kGCm'C
Cuts at: 0 27 122 747
Size: 27 95 625
Fragments arranged by size:
625 95 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 60 747
Size: 60 687
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 466 704 747
Size: 122 344 238 43
Fragments arranged by size:
344 238 122 43
Bpu10I CC'TnA_GC
Cuts at: 0 593 747
Size: 593 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 747
Size: 607 140
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 119 747
Size: 119 628
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 747
Size: 387 360
BsiHKAI G_wGCw'C
Cuts at: 0 122 623 747
Size: 122 501 124
Fragments arranged by size:
501 124 122
Bsp1286I G_dGCh'C
Cuts at: 0 27 122 623 747
Size: 27 95 501 124
Fragments arranged by size:
501 124 95 27
BsrGI T'GTAC_A
Cuts at: 0 732 747
Size: 732 15
BstBI TT'CG_AA
Cuts at: 0 426 747
Size: 426 321
BtgI C'CryG_G
Cuts at: 0 11 747
Size: 11 736
EcoICRI GAG'CTC
Cuts at: 0 621 747
Size: 621 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 466 704 747
Size: 122 344 238 43
Fragments arranged by size:
344 238 122 43
EcoO109I rG'GnC_Cy
Cuts at: 0 533 718 747
Size: 533 185 29
Fragments arranged by size:
533 185 29
HaeII r_GCGC'y
Cuts at: 0 19 747
Size: 19 728
HindIII A'AGCT_T
Cuts at: 0 463 747
Size: 463 284
MfeI C'AATT_G
Cuts at: 0 194 747
Size: 194 553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 747
Size: 360 387
MspA1I CmG'CkG
Cuts at: 0 13 232 682 747
Size: 13 219 450 65
Fragments arranged by size:
450 219 65 13
NspI r_CATG'y
Cuts at: 0 64 423 747
Size: 64 359 324
Fragments arranged by size:
359 324 64
PciI A'CATG_T
Cuts at: 0 60 747
Size: 60 687
PflMI CCAn_nnn'nTGG
Cuts at: 0 658 747
Size: 658 89
PfoI T'CCnGG_A
Cuts at: 0 245 747
Size: 245 502
PpuMI rG'GwC_Cy
Cuts at: 0 718 747
Size: 718 29
PspOMI G'GGCC_C
Cuts at: 0 23 747
Size: 23 724
PvuII CAG'CTG
Cuts at: 0 232 682 747
Size: 232 450 65
Fragments arranged by size:
450 232 65
SacI G_AGCT'C
Cuts at: 0 623 747
Size: 623 124
SacII CC_GC'GG
Cuts at: 0 14 747
Size: 14 733
SmaI CCC'GGG
Cuts at: 0 28 747
Size: 28 719
SmlI C'TyrA_G
Cuts at: 0 622 747
Size: 622 125
SphI G_CATG'C
Cuts at: 0 423 747
Size: 423 324
SrfI GCCC'GGGC
Cuts at: 0 28 747
Size: 28 719
StuI AGG'CCT
Cuts at: 0 743 747
Size: 743 4
StyI C'CwwG_G
Cuts at: 0 652 713 747
Size: 652 61 34
Fragments arranged by size:
652 61 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 260 726 747
Size: 260 466 21
Fragments arranged by size:
466 260 21
TatI w'GTAC_w
Cuts at: 0 732 747
Size: 732 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 265 747
Size: 265 482
XmaI C'CCGG_G
Cuts at: 0 26 747
Size: 26 721
Enzymes that do cut:
AflIII AlwNI ApaI ApaLI ApoI AvaI BanII BbsI
BglI Bme1580I BmrI BpmI Bpu10I BpuEI BseRI BsgI
BsiHKAI Bsp1286I BsrGI BstBI BtgI EcoICRI Eco57MI EcoO109I
HaeII HindIII MfeI MmeI MspA1I NspI PciI PflMI
PfoI PpuMI PspOMI PvuII SacI SacII SmaI SmlI
SphI SrfI StuI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HpaI KasI KpnI MluI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SnaBI SpeI SspI SwaI TstI Tth111I
XbaI XhoI XmnI ZraI