(Linear) (Six-base) MAPSORT of: KIEE1375.seq  Check: 8390  from: 1  to: 636



KIEE1375.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:37  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598     636 
   Size:        598      38
 
AlwNI CAG_nnn'CTG

Cuts at:      0     129     636 
   Size:        129     507
 
ApoI r'AATT_y

Cuts at:      0     194     295     335     496     636 
   Size:        194     101      40     161     140

  Fragments arranged by size: 

                 194     161     140     101      40
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     435     636 
   Size:        435     201
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      89     636 
   Size:         89     547
 
Bme1580I G_kGCm'C

Cuts at:      0     477     636 
   Size:        477     159
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      31     175     636 
   Size:         31     144     461

  Fragments arranged by size: 

                 461     144      31
 
BsiHKAI G_wGCw'C

Cuts at:      0     327     636 
   Size:        327     309
 
Bsp1286I G_dGCh'C

Cuts at:      0     327     477     636 
   Size:        327     150     159

  Fragments arranged by size: 

                 327     159     150
 
BssSI C'ACGA_G

Cuts at:      0     320     636 
   Size:        320     316
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      48     636 
   Size:         48     588
 
BstYI r'GATC_y

Cuts at:      0     347     636 
   Size:        347     289
 
BtgI C'CryG_G

Cuts at:      0      47     636 
   Size:         47     589
 
DraI TTT'AAA

Cuts at:      0     546     636 
   Size:        546      90
 
EarI CTCTTCn'nnn_

Cuts at:      0      11     260     636 
   Size:         11     249     376

  Fragments arranged by size: 

                 376     249      11
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598     636 
   Size:        598      38
 
EcoRI G'AATT_C

Cuts at:      0     295     636 
   Size:        295     341
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     439     636 
   Size:        439     197
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      28      60     636 
   Size:         28      32     576

  Fragments arranged by size: 

                 576      32      28
 
HincII GTy'rAC

Cuts at:      0     222     636 
   Size:        222     414
 
HpaI GTT'AAC

Cuts at:      0     222     636 
   Size:        222     414
 
MslI CAynn'nnrTG

Cuts at:      0      46     187     636 
   Size:         46     141     449

  Fragments arranged by size: 

                 449     141      46
 
MspA1I CmG'CkG

Cuts at:      0     253     636 
   Size:        253     383
 
NcoI C'CATG_G

Cuts at:      0      47     636 
   Size:         47     589
 
PstI C_TGCA'G

Cuts at:      0     120     386     636 
   Size:        120     266     250

  Fragments arranged by size: 

                 266     250     120
 
PvuII CAG'CTG

Cuts at:      0     253     636 
   Size:        253     383
 
ScaI AGT'ACT

Cuts at:      0     508     636 
   Size:        508     128
 
SfcI C'TryA_G

Cuts at:      0     116     382     636 
   Size:        116     266     254

  Fragments arranged by size: 

                 266     254     116
 
StyI C'CwwG_G

Cuts at:      0      47     636 
   Size:         47     589
 
TatI w'GTAC_w

Cuts at:      0     506     636 
   Size:        506     130
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BbsI    BciVI Bme1580I     BsaI  BsiHKAI
 Bsp1286I    BssSI    BstXI    BstYI     BtgI     DraI     EarI  Eco57MI
    EcoRI     FalI    Hin4I   HincII     HpaI     MslI   MspA1I     NcoI
     PstI    PvuII     ScaI     SfcI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI