Restriction map for
pFN21AE1374
(Linear) (Six-base) MAPSORT of: KIEE1374.seq Check: 3147 from: 1 to: 1284
KIEE1374.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AatII G_ACGT'C
Cuts at: 0 1135 1284
Size: 1135 149
ApaLI G'TGCA_C
Cuts at: 0 1075 1284
Size: 1075 209
AvaI C'yCGr_G
Cuts at: 0 196 256 1284
Size: 196 60 1028
Fragments arranged by size:
1028 196 60
BanI G'GyrC_C
Cuts at: 0 300 761 1284
Size: 300 461 523
Fragments arranged by size:
523 461 300
BanII G_rGCy'C
Cuts at: 0 601 692 856 883 1099 1284
Size: 601 91 164 27 216 185
Fragments arranged by size:
601 216 185 164 91 27
BbeI G_GCGC'C
Cuts at: 0 304 1284
Size: 304 980
BbsI GAAGACnn'nnnn_
Cuts at: 0 872 985 1284
Size: 872 113 299
Fragments arranged by size:
872 299 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 145 179 1284
Size: 145 34 1105
Fragments arranged by size:
1105 145 34
BglI GCCn_nnn'nGGC
Cuts at: 0 887 1284
Size: 887 397
BglII A'GATC_T
Cuts at: 0 1221 1284
Size: 1221 63
BlpI GC'TnA_GC
Cuts at: 0 280 1284
Size: 280 1004
Bme1580I G_kGCm'C
Cuts at: 0 764 1079 1284
Size: 764 315 205
Fragments arranged by size:
764 315 205
BmgBI CAC'GTC
Cuts at: 0 69 870 1284
Size: 69 801 414
Fragments arranged by size:
801 414 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 810 1023 1284
Size: 448 362 213 261
Fragments arranged by size:
448 362 261 213
BsaHI Gr'CG_yC
Cuts at: 0 217 301 1132 1284
Size: 217 84 831 152
Fragments arranged by size:
831 217 152 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1093 1123 1284
Size: 1093 30 161
Fragments arranged by size:
1093 161 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 813 1284
Size: 172 641 471
Fragments arranged by size:
641 471 172
BseYI C'CCAG_C
Cuts at: 0 183 783 814 907 1049 1245 1284
Size: 183 600 31 93 142 196 39
Fragments arranged by size:
600 196 183 142 93 39 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 1284
Size: 511 773
BsiEI CG_ry'CG
Cuts at: 0 641 1284
Size: 641 643
BsiHKAI G_wGCw'C
Cuts at: 0 317 436 692 1079 1284
Size: 317 119 256 387 205
Fragments arranged by size:
387 317 256 205 119
Bsp1286I G_dGCh'C
Cuts at: 0 317 436 601 692 764 856 883 1079
Size: 317 119 165 91 72 92 27 196
Cuts at: 1079 1099 1284
Size: 20 185
Fragments arranged by size:
317 196 185 165 119 92 91 72
27 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 1284
Size: 107 1177
BsrBI CCG'CTC
Cuts at: 0 15 1114 1215 1284
Size: 15 1099 101 69
Fragments arranged by size:
1099 101 69 15
BsrFI r'CCGG_y
Cuts at: 0 289 1058 1284
Size: 289 769 226
Fragments arranged by size:
769 289 226
BssSI C'ACGA_G
Cuts at: 0 133 876 918 1284
Size: 133 743 42 366
Fragments arranged by size:
743 366 133 42
BstEII G'GTnAC_C
Cuts at: 0 472 1284
Size: 472 812
BstXI CCAn_nnnn'nTGG
Cuts at: 0 630 1261 1284
Size: 630 631 23
Fragments arranged by size:
631 630 23
BstYI r'GATC_y
Cuts at: 0 1221 1284
Size: 1221 63
BtgI C'CryG_G
Cuts at: 0 713 1284
Size: 713 571
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1053 1284
Size: 1053 231
BtsI GCAGTG_nn'
Cuts at: 0 472 1284
Size: 472 812
EaeI y'GGCC_r
Cuts at: 0 230 512 1175 1284
Size: 230 282 663 109
Fragments arranged by size:
663 282 230 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 385 1284
Size: 385 899
EcoICRI GAG'CTC
Cuts at: 0 690 1284
Size: 690 594
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 810 1023 1284
Size: 448 362 213 261
Fragments arranged by size:
448 362 261 213
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 412 454 1284
Size: 25 387 42 830
Fragments arranged by size:
830 387 42 25
EcoO109I rG'GnC_Cy
Cuts at: 0 54 730 1209 1284
Size: 54 676 479 75
Fragments arranged by size:
676 479 75 54
FspI TGC'GCA
Cuts at: 0 241 464 1284
Size: 241 223 820
Fragments arranged by size:
820 241 223
HaeII r_GCGC'y
Cuts at: 0 304 450 1284
Size: 304 146 834
Fragments arranged by size:
834 304 146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1213 1245 1284
Size: 1213 32 39
Fragments arranged by size:
1213 39 32
KasI G'GCGC_C
Cuts at: 0 300 1284
Size: 300 984
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 1284
Size: 344 940
MscI TGG'CCA
Cuts at: 0 232 1284
Size: 232 1052
MslI CAynn'nnrTG
Cuts at: 0 508 697 1072 1284
Size: 508 189 375 212
Fragments arranged by size:
508 375 212 189
MspA1I CmG'CkG
Cuts at: 0 426 1082 1284
Size: 426 656 202
Fragments arranged by size:
656 426 202
NarI GG'CG_CC
Cuts at: 0 301 1284
Size: 301 983
PasI CC'CwG_GG
Cuts at: 0 744 1284
Size: 744 540
PfoI T'CCnGG_A
Cuts at: 0 647 703 1284
Size: 647 56 581
Fragments arranged by size:
647 581 56
PpuMI rG'GwC_Cy
Cuts at: 0 730 1209 1284
Size: 730 479 75
Fragments arranged by size:
730 479 75
PstI C_TGCA'G
Cuts at: 0 104 758 1284
Size: 104 654 526
Fragments arranged by size:
654 526 104
SacI G_AGCT'C
Cuts at: 0 692 1284
Size: 692 592
SbfI CC_TGCA'GG
Cuts at: 0 104 1284
Size: 104 1180
ScaI AGT'ACT
Cuts at: 0 121 1284
Size: 121 1163
SexAI A'CCwGG_T
Cuts at: 0 88 392 1284
Size: 88 304 892
Fragments arranged by size:
892 304 88
SfcI C'TryA_G
Cuts at: 0 100 754 1284
Size: 100 654 530
Fragments arranged by size:
654 530 100
SfoI GGC'GCC
Cuts at: 0 302 1284
Size: 302 982
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 655 1063 1284
Size: 40 615 408 221
Fragments arranged by size:
615 408 221 40
TatI w'GTAC_w
Cuts at: 0 119 1284
Size: 119 1165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 364 396 1093 1125 1284
Size: 364 32 697 32 159
Fragments arranged by size:
697 364 159 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 394 1284
Size: 394 890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 741 1284
Size: 741 543
ZraI GAC'GTC
Cuts at: 0 1133 1284
Size: 1133 151
Enzymes that do cut:
AatII ApaLI AvaI BanI BanII BbeI BbsI BcgI
BglI BglII BlpI Bme1580I BmgBI BpmI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI
EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NarI PasI
PfoI PpuMI PstI SacI SbfI ScaI SexAI SfcI
SfoI TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EarI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI XbaI XhoI XmaI XmnI