Restriction map for
pFN21AE1372
(Linear) (Six-base) MAPSORT of: KIEE1372.seq Check: 6967 from: 1 to: 1734
KIEE1372.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 465 552 943 1010 1379 1734
Size: 465 87 391 67 369 355
Fragments arranged by size:
465 391 369 355 87 67
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 199 231 1734
Size: 199 32 1503
Fragments arranged by size:
1503 199 32
AlwNI CAG_nnn'CTG
Cuts at: 0 234 1318 1734
Size: 234 1084 416
Fragments arranged by size:
1084 416 234
ApaI G_GGCC'C
Cuts at: 0 1154 1533 1734
Size: 1154 379 201
Fragments arranged by size:
1154 379 201
ApoI r'AATT_y
Cuts at: 0 109 1426 1734
Size: 109 1317 308
Fragments arranged by size:
1317 308 109
AvaI C'yCGr_G
Cuts at: 0 418 1734
Size: 418 1316
BamHI G'GATC_C
Cuts at: 0 1497 1734
Size: 1497 237
BanI G'GyrC_C
Cuts at: 0 519 782 1293 1734
Size: 519 263 511 441
Fragments arranged by size:
519 511 441 263
BanII G_rGCy'C
Cuts at: 0 747 998 1058 1130 1141 1154 1533 1734
Size: 747 251 60 72 11 13 379 201
Fragments arranged by size:
747 379 251 201 72 60 13 11
BbeI G_GCGC'C
Cuts at: 0 786 1734
Size: 786 948
BbsI GAAGACnn'nnnn_
Cuts at: 0 1313 1734
Size: 1313 421
BbvCI CC'TCA_GC
Cuts at: 0 37 1734
Size: 37 1697
Bme1580I G_kGCm'C
Cuts at: 0 1154 1533 1734
Size: 1154 379 201
Fragments arranged by size:
1154 379 201
BmrI ACTGGGnnnn_n'
Cuts at: 0 794 993 1734
Size: 794 199 741
Fragments arranged by size:
794 741 199
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 687 1734
Size: 687 1047
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 751 1408 1635 1734
Size: 605 146 657 227 99
Fragments arranged by size:
657 605 227 146 99
Bpu10I CC'TnA_GC
Cuts at: 0 37 1135 1734
Size: 37 1098 599
Fragments arranged by size:
1098 599 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1602 1734
Size: 1602 132
BsaI GGTCTCn'nnnn_
Cuts at: 0 856 1672 1734
Size: 856 816 62
Fragments arranged by size:
856 816 62
BsaHI Gr'CG_yC
Cuts at: 0 783 1734
Size: 783 951
BsaWI w'CCGG_w
Cuts at: 0 739 1734
Size: 739 995
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 1734
Size: 199 30 1505
Fragments arranged by size:
1505 199 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 676 907 997 1734
Size: 25 651 231 90 737
Fragments arranged by size:
737 651 231 90 25
BseYI C'CCAG_C
Cuts at: 0 79 823 1041 1065 1129 1141 1237 1311
Size: 79 744 218 24 64 12 96 74
Cuts at: 1311 1734
Size: 423
Fragments arranged by size:
744 423 218 96 79 74 64 24
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1734
Size: 922 812
BsmI GAATG_Cn'
Cuts at: 0 1324 1734
Size: 1324 410
Bsp1286I G_dGCh'C
Cuts at: 0 747 998 1058 1130 1141 1154 1533 1734
Size: 747 251 60 72 11 13 379 201
Fragments arranged by size:
747 379 251 201 72 60 13 11
BspEI T'CCGG_A
Cuts at: 0 739 1734
Size: 739 995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 172 944 1734
Size: 172 772 790
Fragments arranged by size:
790 772 172
BsrDI GCAATG_nn'
Cuts at: 0 285 1734
Size: 285 1449
BsrFI r'CCGG_y
Cuts at: 0 317 1734
Size: 317 1417
BstEII G'GTnAC_C
Cuts at: 0 1333 1695 1734
Size: 1333 362 39
Fragments arranged by size:
1333 362 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 280 1734
Size: 280 1454
BstYI r'GATC_y
Cuts at: 0 398 1112 1497 1734
Size: 398 714 385 237
Fragments arranged by size:
714 398 385 237
Bsu36I CC'TnA_GG
Cuts at: 0 184 1289 1339 1524 1734
Size: 184 1105 50 185 210
Fragments arranged by size:
1105 210 185 184 50
BtsI GCAGTG_nn'
Cuts at: 0 260 390 1599 1734
Size: 260 130 1209 135
Fragments arranged by size:
1209 260 135 130
DraIII CAC_nnn'GTG
Cuts at: 0 1252 1734
Size: 1252 482
EaeI y'GGCC_r
Cuts at: 0 1475 1734
Size: 1475 259
EarI CTCTTCn'nnn_
Cuts at: 0 817 986 1355 1734
Size: 817 169 369 379
Fragments arranged by size:
817 379 369 169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 465 552 605 751 943 1010 1379 1408
Size: 465 87 53 146 192 67 369 29
Cuts at: 1408 1635 1734
Size: 227 99
Fragments arranged by size:
465 369 227 192 146 99 87 67
53 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1477 1734
Size: 1477 257
EcoO109I rG'GnC_Cy
Cuts at: 0 1150 1529 1530 1734
Size: 1150 379 1 204
Fragments arranged by size:
1150 379 204 1
HaeII r_GCGC'y
Cuts at: 0 34 786 1734
Size: 34 752 948
Fragments arranged by size:
948 752 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1381 1413 1552 1584 1734
Size: 1381 32 139 32 150
Fragments arranged by size:
1381 150 139 32 32
KasI G'GCGC_C
Cuts at: 0 782 1734
Size: 782 952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 350 1734
Size: 242 108 1384
Fragments arranged by size:
1384 242 108
MscI TGG'CCA
Cuts at: 0 1477 1734
Size: 1477 257
MslI CAynn'nnrTG
Cuts at: 0 278 1734
Size: 278 1456
MspA1I CmG'CkG
Cuts at: 0 771 1076 1109 1315 1589 1734
Size: 771 305 33 206 274 145
Fragments arranged by size:
771 305 274 206 145 33
NaeI GCC'GGC
Cuts at: 0 319 1734
Size: 319 1415
NarI GG'CG_CC
Cuts at: 0 783 1734
Size: 783 951
NdeI CA'TA_TG
Cuts at: 0 468 1734
Size: 468 1266
NgoMIV G'CCGG_C
Cuts at: 0 317 1734
Size: 317 1417
PfoI T'CCnGG_A
Cuts at: 0 202 1734
Size: 202 1532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 985 1017 1734
Size: 199 32 754 32 717
Fragments arranged by size:
754 717 199 32 32
PshAI GACnn'nnGTC
Cuts at: 0 703 1734
Size: 703 1031
PspOMI G'GGCC_C
Cuts at: 0 1150 1529 1734
Size: 1150 379 205
Fragments arranged by size:
1150 379 205
PstI C_TGCA'G
Cuts at: 0 986 1081 1124 1594 1724 1734
Size: 986 95 43 470 130 10
Fragments arranged by size:
986 470 130 95 43 10
PvuII CAG'CTG
Cuts at: 0 771 1076 1315 1589 1734
Size: 771 305 239 274 145
Fragments arranged by size:
771 305 274 239 145
SapI GCTCTTCn'nnn_
Cuts at: 0 817 986 1734
Size: 817 169 748
Fragments arranged by size:
817 748 169
SbfI CC_TGCA'GG
Cuts at: 0 1124 1734
Size: 1124 610
SexAI A'CCwGG_T
Cuts at: 0 1691 1734
Size: 1691 43
SfcI C'TryA_G
Cuts at: 0 222 982 1077 1120 1590 1720 1734
Size: 222 760 95 43 470 130 14
Fragments arranged by size:
760 470 222 130 95 43 14
SfoI GGC'GCC
Cuts at: 0 784 1734
Size: 784 950
SmlI C'TyrA_G
Cuts at: 0 1581 1734
Size: 1581 153
StuI AGG'CCT
Cuts at: 0 898 1734
Size: 898 836
StyI C'CwwG_G
Cuts at: 0 260 1262 1416 1478 1734
Size: 260 1002 154 62 256
Fragments arranged by size:
1002 260 256 154 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 153 1734
Size: 153 1581
Tth111I GACn'n_nGTC
Cuts at: 0 234 1693 1734
Size: 234 1459 41
Fragments arranged by size:
1459 234 41
XbaI T'CTAG_A
Cuts at: 0 1503 1734
Size: 1503 231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 444 1198 1541 1734
Size: 444 754 343 193
Fragments arranged by size:
754 444 343 193
Enzymes that do cut:
AcuI AloI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsmI Bsp1286I BspEI BspMI BsrDI BsrFI BstEII
BstXI BstYI Bsu36I BtsI DraIII EaeI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV PfoI PpiI PshAI
PspOMI PstI PvuII SapI SbfI SexAI SfcI SfoI
SmlI StuI StyI TaqII Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI
AvrII BaeI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BsaAI BsaBI BsiEI BsiHKAI BsiWI
BsmBI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I BtgI BtgZI ClaI CspCI DraI DrdI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NcoI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpuMI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI XhoI XmaI XmnI ZraI