Restriction map for
pFN21AE1371
(Linear) (Six-base) MAPSORT of: KIEE1371.seq Check: 8701 from: 1 to: 585
KIEE1371.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AfeI AGC'GCT
Cuts at: 0 199 585
Size: 199 386
AlwNI CAG_nnn'CTG
Cuts at: 0 42 585
Size: 42 543
ApaI G_GGCC'C
Cuts at: 0 245 585
Size: 245 340
AvaI C'yCGr_G
Cuts at: 0 164 413 537 585
Size: 164 249 124 48
Fragments arranged by size:
249 164 124 48
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 99 132 585
Size: 99 33 453
Fragments arranged by size:
453 99 33
BanI G'GyrC_C
Cuts at: 0 115 294 436 585
Size: 115 179 142 149
Fragments arranged by size:
179 149 142 115
BanII G_rGCy'C
Cuts at: 0 187 245 407 585
Size: 187 58 162 178
Fragments arranged by size:
187 178 162 58
BglI GCCn_nnn'nGGC
Cuts at: 0 25 340 585
Size: 25 315 245
Fragments arranged by size:
315 245 25
Bme1580I G_kGCm'C
Cuts at: 0 245 299 585
Size: 245 54 286
Fragments arranged by size:
286 245 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 96 585
Size: 96 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 585
Size: 175 410
BsaBI GATnn'nnATC
Cuts at: 0 61 585
Size: 61 524
BsaHI Gr'CG_yC
Cuts at: 0 46 394 585
Size: 46 348 191
Fragments arranged by size:
348 191 46
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 520 523 585
Size: 520 3 62
Fragments arranged by size:
520 62 3
BseYI C'CCAG_C
Cuts at: 0 88 200 454 499 585
Size: 88 112 254 45 86
Fragments arranged by size:
254 112 88 86 45
BsiEI CG_ry'CG
Cuts at: 0 424 482 585
Size: 424 58 103
Fragments arranged by size:
424 103 58
Bsp1286I G_dGCh'C
Cuts at: 0 187 245 299 407 585
Size: 187 58 54 108 178
Fragments arranged by size:
187 178 108 58 54
BsrBI CCG'CTC
Cuts at: 0 353 420 585
Size: 353 67 165
Fragments arranged by size:
353 165 67
BsrDI GCAATG_nn'
Cuts at: 0 344 585
Size: 344 241
BsrFI r'CCGG_y
Cuts at: 0 301 585
Size: 301 284
BstAPI GCAn_nnn'nTGC
Cuts at: 0 285 585
Size: 285 300
BstEII G'GTnAC_C
Cuts at: 0 567 585
Size: 567 18
BtgI C'CryG_G
Cuts at: 0 191 224 469 585
Size: 191 33 245 116
Fragments arranged by size:
245 191 116 33
BtsI GCAGTG_nn'
Cuts at: 0 394 585
Size: 394 191
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 306 341 585
Size: 306 35 244
Fragments arranged by size:
306 244 35
DraIII CAC_nnn'GTG
Cuts at: 0 104 585
Size: 104 481
EaeI y'GGCC_r
Cuts at: 0 80 314 341 421 479 585
Size: 80 234 27 80 58 106
Fragments arranged by size:
234 106 80 80 58 27
EagI C'GGCC_G
Cuts at: 0 421 479 585
Size: 421 58 106
Fragments arranged by size:
421 106 58
EarI CTCTTCn'nnn_
Cuts at: 0 166 585
Size: 166 419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 585
Size: 175 410
EcoNI CCTnn'n_nnAGG
Cuts at: 0 249 585
Size: 249 336
EcoO109I rG'GnC_Cy
Cuts at: 0 242 532 544 585
Size: 242 290 12 41
Fragments arranged by size:
290 242 41 12
HaeII r_GCGC'y
Cuts at: 0 43 96 201 585
Size: 43 53 105 384
Fragments arranged by size:
384 105 53 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 425 457 585
Size: 425 32 128
Fragments arranged by size:
425 128 32
HincII GTy'rAC
Cuts at: 0 555 585
Size: 555 30
MscI TGG'CCA
Cuts at: 0 82 316 343 585
Size: 82 234 27 242
Fragments arranged by size:
242 234 82 27
MslI CAynn'nnrTG
Cuts at: 0 565 585
Size: 565 20
MspA1I CmG'CkG
Cuts at: 0 88 471 585
Size: 88 383 114
Fragments arranged by size:
383 114 88
NaeI GCC'GGC
Cuts at: 0 303 585
Size: 303 282
NcoI C'CATG_G
Cuts at: 0 191 585
Size: 191 394
NgoMIV G'CCGG_C
Cuts at: 0 301 585
Size: 301 284
NotI GC'GGCC_GC
Cuts at: 0 421 585
Size: 421 164
PpuMI rG'GwC_Cy
Cuts at: 0 532 544 585
Size: 532 12 41
Fragments arranged by size:
532 41 12
PspOMI G'GGCC_C
Cuts at: 0 241 585
Size: 241 344
PvuII CAG'CTG
Cuts at: 0 88 585
Size: 88 497
SacII CC_GC'GG
Cuts at: 0 472 585
Size: 472 113
SanDI GG'GwC_CC
Cuts at: 0 532 585
Size: 532 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 25 585
Size: 25 560
SmaI CCC'GGG
Cuts at: 0 166 415 539 585
Size: 166 249 124 46
Fragments arranged by size:
249 166 124 46
StyI C'CwwG_G
Cuts at: 0 191 324 585
Size: 191 133 261
Fragments arranged by size:
261 191 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 115 585
Size: 115 470
TatI w'GTAC_w
Cuts at: 0 212 585
Size: 212 373
Tth111I GACn'n_nGTC
Cuts at: 0 577 585
Size: 577 8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 109 585
Size: 109 476
XmaI C'CCGG_G
Cuts at: 0 164 413 537 585
Size: 164 249 124 48
Fragments arranged by size:
249 164 124 48
XmnI GAAnn'nnTTC
Cuts at: 0 235 585
Size: 235 350
Enzymes that do cut:
AfeI AlwNI ApaI AvaI BaeI BanI BanII BglI
Bme1580I BmrI BpmI BsaBI BsaHI BseRI BseYI BsiEI
Bsp1286I BsrBI BsrDI BsrFI BstAPI BstEII BtgI BtsI
CspCI DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII MscI MslI MspA1I NaeI NcoI
NgoMIV NotI PpuMI PspOMI PvuII SacII SanDI SfiI
SmaI StyI TaqII TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BsgI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI BssHII
BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
DraI DrdI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NdeI NheI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI XbaI XhoI ZraI