Restriction map for
pFN21AE1368
(Linear) (Six-base) MAPSORT of: KIEE1368.seq Check: 5120 from: 1 to: 2016
KIEE1368.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 180 495 676 986 1376 2016
Size: 180 315 181 310 390 640
Fragments arranged by size:
640 390 315 310 181 180
AccI GT'mk_AC
Cuts at: 0 91 461 2016
Size: 91 370 1555
Fragments arranged by size:
1555 370 91
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 661 1441 2016
Size: 580 81 780 575
Fragments arranged by size:
780 580 575 81
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 787 1077 1696 2016
Size: 787 290 619 320
Fragments arranged by size:
787 619 320 290
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 205 237 418 450 883 904 915 936
Size: 205 32 181 32 433 21 11 21
Cuts at: 936 2016
Size: 1080
Fragments arranged by size:
1080 433 205 181 32 32 21 21
11
AlwNI CAG_nnn'CTG
Cuts at: 0 204 654 665 2016
Size: 204 450 11 1351
Fragments arranged by size:
1351 450 204 11
ApaI G_GGCC'C
Cuts at: 0 1188 1654 2016
Size: 1188 466 362
Fragments arranged by size:
1188 466 362
ApoI r'AATT_y
Cuts at: 0 1096 2016
Size: 1096 920
AvaI C'yCGr_G
Cuts at: 0 692 1187 1236 1513 1645 2016
Size: 692 495 49 277 132 371
Fragments arranged by size:
692 495 371 277 132 49
AvrII C'CTAG_G
Cuts at: 0 1303 2016
Size: 1303 713
BamHI G'GATC_C
Cuts at: 0 779 2016
Size: 779 1237
BanI G'GyrC_C
Cuts at: 0 257 688 1333 1861 2016
Size: 257 431 645 528 155
Fragments arranged by size:
645 528 431 257 155
BanII G_rGCy'C
Cuts at: 0 322 743 818 875 944 1188 1225 1382
Size: 322 421 75 57 69 244 37 157
Cuts at: 1382 1654 2016
Size: 272 362
Fragments arranged by size:
421 362 322 272 244 157 75 69
57 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 1446 2016
Size: 1446 570
BclI T'GATC_A
Cuts at: 0 656 2016
Size: 656 1360
BfrBI ATG'CAT
Cuts at: 0 1348 2016
Size: 1348 668
BglI GCCn_nnn'nGGC
Cuts at: 0 1489 2016
Size: 1489 527
BlpI GC'TnA_GC
Cuts at: 0 673 1801 2016
Size: 673 1128 215
Fragments arranged by size:
1128 673 215
Bme1580I G_kGCm'C
Cuts at: 0 693 1188 1194 1654 1880 2016
Size: 693 495 6 460 226 136
Fragments arranged by size:
693 495 460 226 136 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 99 1182 1694 2016
Size: 99 1083 512 322
Fragments arranged by size:
1083 512 322 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 840 871 2016
Size: 727 113 31 1145
Fragments arranged by size:
1145 727 113 31
Bpu10I CC'TnA_GC
Cuts at: 0 1477 2016
Size: 1477 539
BsaI GGTCTCn'nnnn_
Cuts at: 0 1116 1995 2016
Size: 1116 879 21
Fragments arranged by size:
1116 879 21
BsaAI yAC'GTr
Cuts at: 0 125 2016
Size: 125 1891
BsaHI Gr'CG_yC
Cuts at: 0 22 47 2016
Size: 22 25 1969
Fragments arranged by size:
1969 25 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 304 334 390 418 420 448 904 934
Size: 304 30 56 28 2 28 456 30
Cuts at: 934 2016
Size: 1082
Fragments arranged by size:
1082 456 304 56 30 30 28 28
2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 60 925 928 931 2016
Size: 60 865 3 3 1085
Fragments arranged by size:
1085 865 60 3 3
BseYI C'CCAG_C
Cuts at: 0 207 296 353 605 699 881 1084 1224
Size: 207 89 57 252 94 182 203 140
Cuts at: 1224 1408 1968 2016
Size: 184 560 48
Fragments arranged by size:
560 252 207 203 184 182 140 94
89 57 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 1249 1570 2016
Size: 1144 105 321 446
Fragments arranged by size:
1144 446 321 105
BsiEI CG_ry'CG
Cuts at: 0 1356 2016
Size: 1356 660
BsiHKAI G_wGCw'C
Cuts at: 0 491 614 674 743 875 944 1382 2016
Size: 491 123 60 69 132 69 438 634
Fragments arranged by size:
634 491 438 132 123 69 69 60
Bsp1286I G_dGCh'C
Cuts at: 0 322 491 614 674 693 743 818 875
Size: 322 169 123 60 19 50 75 57
Cuts at: 875 944 1188 1194 1225 1382 1654 1880 2016
Size: 69 244 6 31 157 272 226 136
Fragments arranged by size:
322 272 244 226 169 157 136 123
75 69 60 57 50 31 19 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 180 495 676 740 986 1376 1898 2016
Size: 180 315 181 64 246 390 522 118
Fragments arranged by size:
522 390 315 246 181 180 118 64
BsrDI GCAATG_nn'
Cuts at: 0 1133 2016
Size: 1133 883
BsrFI r'CCGG_y
Cuts at: 0 24 532 797 970 1694 1838 2016
Size: 24 508 265 173 724 144 178
Fragments arranged by size:
724 508 265 178 173 144 24
BsrGI T'GTAC_A
Cuts at: 0 1130 2016
Size: 1130 886
BstAPI GCAn_nnn'nTGC
Cuts at: 0 204 1340 2016
Size: 204 1136 676
Fragments arranged by size:
1136 676 204
BstEII G'GTnAC_C
Cuts at: 0 363 2016
Size: 363 1653
BstXI CCAn_nnnn'nTGG
Cuts at: 0 605 2016
Size: 605 1411
BstYI r'GATC_y
Cuts at: 0 779 1150 2016
Size: 779 371 866
Fragments arranged by size:
866 779 371
Bsu36I CC'TnA_GG
Cuts at: 0 925 2016
Size: 925 1091
BtgI C'CryG_G
Cuts at: 0 62 178 474 1325 2016
Size: 62 116 296 851 691
Fragments arranged by size:
851 691 296 116 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1801 1908 2016
Size: 1801 107 108
Fragments arranged by size:
1801 108 107
BtsI GCAGTG_nn'
Cuts at: 0 1499 1689 1777 2009 2016
Size: 1499 190 88 232 7
Fragments arranged by size:
1499 232 190 88 7
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1354 1389 2016
Size: 1354 35 627
Fragments arranged by size:
1354 627 35
DraIII CAC_nnn'GTG
Cuts at: 0 1340 2016
Size: 1340 676
DrdI GACnn_nn'nnGTC
Cuts at: 0 1360 2016
Size: 1360 656
EaeI y'GGCC_r
Cuts at: 0 972 1496 1575 2016
Size: 972 524 79 441
Fragments arranged by size:
972 524 441 79
EarI CTCTTCn'nnn_
Cuts at: 0 82 244 2016
Size: 82 162 1772
Fragments arranged by size:
1772 162 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 526 1183 2016
Size: 526 657 833
Fragments arranged by size:
833 657 526
EcoICRI GAG'CTC
Cuts at: 0 741 873 942 1380 2016
Size: 741 132 69 438 636
Fragments arranged by size:
741 636 438 132 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 661 727 840 871 1441 2016
Size: 580 81 66 113 31 570 575
Fragments arranged by size:
580 575 570 113 81 66 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 268 1273 1984 2016
Size: 268 1005 711 32
Fragments arranged by size:
1005 711 268 32
EcoO109I rG'GnC_Cy
Cuts at: 0 479 1031 1308 1650 1651 1743 1907 2016
Size: 479 552 277 342 1 92 164 109
Fragments arranged by size:
552 479 342 277 164 109 92 1
FseI GG_CCGG'CC
Cuts at: 0 974 2016
Size: 974 1042
HaeII r_GCGC'y
Cuts at: 0 1024 1484 2016
Size: 1024 460 532
Fragments arranged by size:
1024 532 460
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 862 894 1930 1962 2016
Size: 862 32 1036 32 54
Fragments arranged by size:
1036 862 54 32 32
MscI TGG'CCA
Cuts at: 0 1498 2016
Size: 1498 518
MslI CAynn'nnrTG
Cuts at: 0 2002 2016
Size: 2002 14
MspA1I CmG'CkG
Cuts at: 0 64 99 416 605 630 662 859 1076
Size: 64 35 317 189 25 32 197 217
Cuts at: 1076 1664 1797 2016
Size: 588 133 219
Fragments arranged by size:
588 317 219 217 197 189 133 64
35 32 25
NaeI GCC'GGC
Cuts at: 0 26 799 972 1696 1840 2016
Size: 26 773 173 724 144 176
Fragments arranged by size:
773 724 176 173 144 26
NcoI C'CATG_G
Cuts at: 0 474 2016
Size: 474 1542
NgoMIV G'CCGG_C
Cuts at: 0 24 797 970 1694 1838 2016
Size: 24 773 173 724 144 178
Fragments arranged by size:
773 724 178 173 144 24
NsiI A_TGCA'T
Cuts at: 0 1350 2016
Size: 1350 666
PasI CC'CwG_GG
Cuts at: 0 791 2016
Size: 791 1225
PflMI CCAn_nnn'nTGG
Cuts at: 0 1100 1257 1753 2016
Size: 1100 157 496 263
Fragments arranged by size:
1100 496 263 157
PfoI T'CCnGG_A
Cuts at: 0 41 263 282 887 2016
Size: 41 222 19 605 1129
Fragments arranged by size:
1129 605 222 41 19
PmlI CAC'GTG
Cuts at: 0 125 2016
Size: 125 1891
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 904 936 1591 1623 2016
Size: 418 32 454 32 655 32 393
Fragments arranged by size:
655 454 418 393 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 479 1308 1743 2016
Size: 479 829 435 273
Fragments arranged by size:
829 479 435 273
PshAI GACnn'nnGTC
Cuts at: 0 650 2016
Size: 650 1366
PspOMI G'GGCC_C
Cuts at: 0 1184 1650 2016
Size: 1184 466 366
Fragments arranged by size:
1184 466 366
PstI C_TGCA'G
Cuts at: 0 248 983 1148 1790 1860 2016
Size: 248 735 165 642 70 156
Fragments arranged by size:
735 642 248 165 156 70
PvuII CAG'CTG
Cuts at: 0 630 662 2016
Size: 630 32 1354
Fragments arranged by size:
1354 630 32
SacI G_AGCT'C
Cuts at: 0 743 875 944 1382 2016
Size: 743 132 69 438 634
Fragments arranged by size:
743 634 438 132 69
SacII CC_GC'GG
Cuts at: 0 65 2016
Size: 65 1951
SapI GCTCTTCn'nnn_
Cuts at: 0 82 244 2016
Size: 82 162 1772
Fragments arranged by size:
1772 162 82
SbfI CC_TGCA'GG
Cuts at: 0 1860 2016
Size: 1860 156
SexAI A'CCwGG_T
Cuts at: 0 269 2016
Size: 269 1747
SfcI C'TryA_G
Cuts at: 0 244 979 1144 1786 1856 2016
Size: 244 735 165 642 70 160
Fragments arranged by size:
735 642 244 165 160 70
SgrAI Cr'CCGG_yG
Cuts at: 0 24 2016
Size: 24 1992
SmaI CCC'GGG
Cuts at: 0 694 1189 1238 1515 2016
Size: 694 495 49 277 501
Fragments arranged by size:
694 501 495 277 49
SrfI GCCC'GGGC
Cuts at: 0 1189 2016
Size: 1189 827
StyI C'CwwG_G
Cuts at: 0 467 474 1303 2016
Size: 467 7 829 713
Fragments arranged by size:
829 713 467 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 345 894 1189 1629 2016
Size: 345 549 295 440 387
Fragments arranged by size:
549 440 387 345 295
TatI w'GTAC_w
Cuts at: 0 1130 2016
Size: 1130 886
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 109 141 341 373 2016
Size: 109 32 200 32 1643
Fragments arranged by size:
1643 200 109 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 271 2016
Size: 271 1745
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1323 1491 2016
Size: 1323 168 525
Fragments arranged by size:
1323 525 168
XmaI C'CCGG_G
Cuts at: 0 692 1187 1236 1513 2016
Size: 692 495 49 277 503
Fragments arranged by size:
692 503 495 277 49
Enzymes that do cut:
AarI AccI AcuI AlfI AloI AlwNI ApaI ApoI
AvaI AvrII BamHI BanI BanII BbsI BclI BfrBI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII
Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV NsiI
PasI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuII SacI SacII SapI SbfI SexAI SfcI
SgrAI SmaI SrfI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI ApaLI AscI AseI AsiSI BaeI BbeI BbvCI
BcgI BciVI BglII BmgBI BmtI BplI BpuEI BsaBI
BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII
BssSI BstBI BstZ17I ClaI DraI EagI EcoRI EcoRV
FalI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NdeI NheI NotI NruI
NspI PacI PciI PmeI PsiI PspXI PsrI PvuI
RsrII SalI SanDI ScaI SfiI SfoI SgfI SmlI
SnaBI SpeI SphI SspI StuI SwaI XbaI XhoI
XmnI ZraI