Restriction map for
pFN21AE1359
(Linear) (Six-base) MAPSORT of: KIEE1359.seq Check: 5000 from: 1 to: 1230
KIEE1359.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AccI GT'mk_AC
Cuts at: 0 755 891 1230
Size: 755 136 339
Fragments arranged by size:
755 339 136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1029 1057 1230
Size: 1029 28 173
Fragments arranged by size:
1029 173 28
AflII C'TTAA_G
Cuts at: 0 381 709 1230
Size: 381 328 521
Fragments arranged by size:
521 381 328
AgeI A'CCGG_T
Cuts at: 0 521 1230
Size: 521 709
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 228 653 1230
Size: 228 425 577
Fragments arranged by size:
577 425 228
ApoI r'AATT_y
Cuts at: 0 674 877 1230
Size: 674 203 353
Fragments arranged by size:
674 353 203
AseI AT'TA_AT
Cuts at: 0 691 1230
Size: 691 539
AvaI C'yCGr_G
Cuts at: 0 1118 1230
Size: 1118 112
BamHI G'GATC_C
Cuts at: 0 120 1230
Size: 120 1110
BanII G_rGCy'C
Cuts at: 0 1030 1178 1230
Size: 1030 148 52
Fragments arranged by size:
1030 148 52
BciVI GTATCCnnnnn_n'
Cuts at: 0 818 1230
Size: 818 412
BmrI ACTGGGnnnn_n'
Cuts at: 0 1163 1230
Size: 1163 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 1230
Size: 66 1164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 625 1230
Size: 421 204 605
Fragments arranged by size:
605 421 204
BsaI GGTCTCn'nnnn_
Cuts at: 0 484 1230
Size: 484 746
BsaHI Gr'CG_yC
Cuts at: 0 1052 1230
Size: 1052 178
BsaWI w'CCGG_w
Cuts at: 0 521 1230
Size: 521 709
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 1230
Size: 199 30 1001
Fragments arranged by size:
1001 199 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1151 1168 1230
Size: 1151 17 62
Fragments arranged by size:
1151 62 17
BseYI C'CCAG_C
Cuts at: 0 326 812 1230
Size: 326 486 418
Fragments arranged by size:
486 418 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1230
Size: 224 1006
BsiHKAI G_wGCw'C
Cuts at: 0 1030 1230
Size: 1030 200
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1012 1230
Size: 1012 218
Bsp1286I G_dGCh'C
Cuts at: 0 1030 1178 1230
Size: 1030 148 52
Fragments arranged by size:
1030 148 52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 753 1230
Size: 753 477
BsrBI CCG'CTC
Cuts at: 0 21 36 1196 1230
Size: 21 15 1160 34
Fragments arranged by size:
1160 34 21 15
BsrFI r'CCGG_y
Cuts at: 0 521 1230
Size: 521 709
BstAPI GCAn_nnn'nTGC
Cuts at: 0 202 1230
Size: 202 1028
BstYI r'GATC_y
Cuts at: 0 120 846 1230
Size: 120 726 384
Fragments arranged by size:
726 384 120
BstZ17I GTA'TAC
Cuts at: 0 756 892 1230
Size: 756 136 338
Fragments arranged by size:
756 338 136
BtgI C'CryG_G
Cuts at: 0 683 1004 1230
Size: 683 321 226
Fragments arranged by size:
683 321 226
DrdI GACnn_nn'nnGTC
Cuts at: 0 738 1230
Size: 738 492
EaeI y'GGCC_r
Cuts at: 0 137 1230
Size: 137 1093
EarI CTCTTCn'nnn_
Cuts at: 0 82 419 1230
Size: 82 337 811
Fragments arranged by size:
811 337 82
EcoICRI GAG'CTC
Cuts at: 0 1028 1230
Size: 1028 202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 1029 1057 1230
Size: 66 963 28 173
Fragments arranged by size:
963 173 66 28
EcoO109I rG'GnC_Cy
Cuts at: 0 705 1230
Size: 705 525
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 396 1230
Size: 396 834
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 329 361 1230
Size: 199 32 98 32 869
Fragments arranged by size:
869 199 98 32 32
HincII GTy'rAC
Cuts at: 0 867 1230
Size: 867 363
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 1230
Size: 824 406
MscI TGG'CCA
Cuts at: 0 139 1230
Size: 139 1091
MspA1I CmG'CkG
Cuts at: 0 312 1214 1230
Size: 312 902 16
Fragments arranged by size:
902 312 16
NcoI C'CATG_G
Cuts at: 0 683 1004 1230
Size: 683 321 226
Fragments arranged by size:
683 321 226
NspI r_CATG'y
Cuts at: 0 762 1230
Size: 762 468
PfoI T'CCnGG_A
Cuts at: 0 82 857 1230
Size: 82 775 373
Fragments arranged by size:
775 373 82
PsiI TTA'TAA
Cuts at: 0 1012 1230
Size: 1012 218
PstI C_TGCA'G
Cuts at: 0 422 1230
Size: 422 808
SacI G_AGCT'C
Cuts at: 0 1030 1230
Size: 1030 200
SfcI C'TryA_G
Cuts at: 0 418 1230
Size: 418 812
SmlI C'TyrA_G
Cuts at: 0 381 400 604 709 1230
Size: 381 19 204 105 521
Fragments arranged by size:
521 381 204 105 19
StyI C'CwwG_G
Cuts at: 0 181 683 1004 1230
Size: 181 502 321 226
Fragments arranged by size:
502 321 226 181
TatI w'GTAC_w
Cuts at: 0 318 1230
Size: 318 912
XmnI GAAnn'nnTTC
Cuts at: 0 1042 1230
Size: 1042 188
Enzymes that do cut:
AccI AcuI AflII AgeI AlfI ApoI AseI AvaI
BamHI BanII BciVI BmrI BpmI BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrFI BstAPI BstYI BstZ17I BtgI DrdI
EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII
MmeI MscI MspA1I NcoI NspI PfoI PsiI PstI
SacI SfcI SmlI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AhdI AleI
AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI
BanI BbeI BbsI BbvCI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaAI
BsaBI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstXI Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII EagI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI ZraI