(Linear) (Six-base) MAPSORT of: KIEE1358.seq  Check: 9672  from: 1  to: 441



KIEE1358.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:51  ..

AgeI A'CCGG_T

Cuts at:      0     316     441 
   Size:        316     125
 
AlwNI CAG_nnn'CTG

Cuts at:      0     121     298     441 
   Size:        121     177     143

  Fragments arranged by size: 

                 177     143     121
 
AvrII C'CTAG_G

Cuts at:      0     360     441 
   Size:        360      81
 
BanI G'GyrC_C

Cuts at:      0     364     441 
   Size:        364      77
 
Bme1580I G_kGCm'C

Cuts at:      0     156     369     441 
   Size:        156     213      72

  Fragments arranged by size: 

                 213     156      72
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     131     441 
   Size:        131     310
 
BsaBI GATnn'nnATC

Cuts at:      0     398     441 
   Size:        398      43
 
BsaWI w'CCGG_w

Cuts at:      0     316     441 
   Size:        316     125
 
BseYI C'CCAG_C

Cuts at:      0     111     208     441 
   Size:        111      97     233

  Fragments arranged by size: 

                 233     111      97
 
Bsp1286I G_dGCh'C

Cuts at:      0     156     369     441 
   Size:        156     213      72

  Fragments arranged by size: 

                 213     156      72
 
BsrFI r'CCGG_y

Cuts at:      0     316     441 
   Size:        316     125
 
BsrGI T'GTAC_A

Cuts at:      0     254     441 
   Size:        254     187
 
BstEII G'GTnAC_C

Cuts at:      0     125     441 
   Size:        125     316
 
EaeI y'GGCC_r

Cuts at:      0       2     264     441 
   Size:          2     262     177

  Fragments arranged by size: 

                 262     177       2
 
HincII GTy'rAC

Cuts at:      0     353     441 
   Size:        353      88
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     348     441 
   Size:         57     291      93

  Fragments arranged by size: 

                 291      93      57
 
PasI CC'CwG_GG

Cuts at:      0     369     441 
   Size:        369      72
 
SspI AAT'ATT

Cuts at:      0     414     441 
   Size:        414      27
 
StyI C'CwwG_G

Cuts at:      0     360     441 
   Size:        360      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     200     441 
   Size:        200     241
 
TatI w'GTAC_w

Cuts at:      0     254     441 
   Size:        254     187
 
Tth111I GACn'n_nGTC

Cuts at:      0     329     441 
   Size:        329     112
 

 Enzymes that do cut:

     AgeI    AlwNI    AvrII     BanI Bme1580I     BmrI    BsaBI    BsaWI
    BseYI Bsp1286I    BsrFI    BsrGI   BstEII     EaeI   HincII     MmeI
     PasI     SspI     StyI    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI   BssHII
    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI