(Linear) (Six-base) MAPSORT of: KIEE1357.seq  Check: 7989  from: 1  to: 456



KIEE1357.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:37  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     200     259     456 
   Size:        149      51      59     197

  Fragments arranged by size: 

                 197     149      59      51
 
AlwNI CAG_nnn'CTG

Cuts at:      0     333     456 
   Size:        333     123
 
ApoI r'AATT_y

Cuts at:      0     172     456 
   Size:        172     284
 
BamHI G'GATC_C

Cuts at:      0      54     456 
   Size:         54     402
 
BanI G'GyrC_C

Cuts at:      0      20     456 
   Size:         20     436
 
BanII G_rGCy'C

Cuts at:      0      81     456 
   Size:         81     375
 
BbeI G_GCGC'C

Cuts at:      0      24     456 
   Size:         24     432
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     299     456 
   Size:        299     157
 
BlpI GC'TnA_GC

Cuts at:      0     380     456 
   Size:        380      76
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     456 
   Size:         44     412
 
BsaHI Gr'CG_yC

Cuts at:      0      21     456 
   Size:         21     435
 
Bsp1286I G_dGCh'C

Cuts at:      0      81     456 
   Size:         81     375
 
BstYI r'GATC_y

Cuts at:      0      54     456 
   Size:         54     402
 
DraI TTT'AAA

Cuts at:      0     159     456 
   Size:        159     297
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     149     200     259     456 
   Size:         44     105      51      59     197

  Fragments arranged by size: 

                 197     105      59      51      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0     134     456 
   Size:        134     322
 
EcoRI G'AATT_C

Cuts at:      0     172     456 
   Size:        172     284
 
HaeII r_GCGC'y

Cuts at:      0      24     456 
   Size:         24     432
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     216     248     456 
   Size:        216      32     208

  Fragments arranged by size: 

                 216     208      32
 
KasI G'GCGC_C

Cuts at:      0      20     456 
   Size:         20     436
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     316     317     456 
   Size:        265      51       1     139

  Fragments arranged by size: 

                 265     139      51       1
 
MslI CAynn'nnrTG

Cuts at:      0     100     236     456 
   Size:        100     136     220

  Fragments arranged by size: 

                 220     136     100
 
MspA1I CmG'CkG

Cuts at:      0      74     384     456 
   Size:         74     310      72

  Fragments arranged by size: 

                 310      74      72
 
NarI GG'CG_CC

Cuts at:      0      21     456 
   Size:         21     435
 
PpuMI rG'GwC_Cy

Cuts at:      0     134     456 
   Size:        134     322
 
PvuII CAG'CTG

Cuts at:      0     384     456 
   Size:        384      72
 
SanDI GG'GwC_CC

Cuts at:      0     134     456 
   Size:        134     322
 
SfcI C'TryA_G

Cuts at:      0     203     456 
   Size:        203     253
 
SfoI GGC'GCC

Cuts at:      0      22     456 
   Size:         22     434
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    BamHI     BanI    BanII     BbeI     BbsI
     BlpI     BpmI    BsaHI Bsp1286I    BstYI     DraI  Eco57MI EcoO109I
    EcoRI    HaeII    Hin4I     KasI     MmeI     MslI   MspA1I     NarI
    PpuMI    PvuII    SanDI     SfcI     SfoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI