Restriction map for
pFN21AE1353
(Linear) (Six-base) MAPSORT of: KIEE1353.seq Check: 2293 from: 1 to: 621
KIEE1353.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AccI GT'mk_AC
Cuts at: 0 124 621
Size: 124 497
Acc65I G'GTAC_C
Cuts at: 0 314 621
Size: 314 307
AfeI AGC'GCT
Cuts at: 0 229 621
Size: 229 392
AvaI C'yCGr_G
Cuts at: 0 146 318 621
Size: 146 172 303
Fragments arranged by size:
303 172 146
BanI G'GyrC_C
Cuts at: 0 159 184 204 314 621
Size: 159 25 20 110 307
Fragments arranged by size:
307 159 110 25 20
BanII G_rGCy'C
Cuts at: 0 7 179 621
Size: 7 172 442
Fragments arranged by size:
442 172 7
BglI GCCn_nnn'nGGC
Cuts at: 0 276 621
Size: 276 345
Bme1580I G_kGCm'C
Cuts at: 0 164 187 209 272 621
Size: 164 23 22 63 349
Fragments arranged by size:
349 164 63 23 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 284 621
Size: 284 337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 491 621
Size: 280 211 130
Fragments arranged by size:
280 211 130
BsaBI GATnn'nnATC
Cuts at: 0 298 621
Size: 298 323
BsaHI Gr'CG_yC
Cuts at: 0 587 621
Size: 587 34
BsaWI w'CCGG_w
Cuts at: 0 290 311 621
Size: 290 21 310
Fragments arranged by size:
310 290 21
BseYI C'CCAG_C
Cuts at: 0 71 208 330 434 455 483 538 621
Size: 71 137 122 104 21 28 55 83
Fragments arranged by size:
137 122 104 83 71 55 28 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 518 621
Size: 244 274 103
Fragments arranged by size:
274 244 103
BsiHKAI G_wGCw'C
Cuts at: 0 179 477 621
Size: 179 298 144
Fragments arranged by size:
298 179 144
BsmI GAATG_Cn'
Cuts at: 0 373 621
Size: 373 248
Bsp1286I G_dGCh'C
Cuts at: 0 7 164 179 187 209 272 477 621
Size: 7 157 15 8 22 63 205 144
Fragments arranged by size:
205 157 144 63 22 15 8 7
BspEI T'CCGG_A
Cuts at: 0 290 621
Size: 290 331
BspHI T'CATG_A
Cuts at: 0 305 621
Size: 305 316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 145 576 621
Size: 145 431 45
Fragments arranged by size:
431 145 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 201 621
Size: 201 420
BstYI r'GATC_y
Cuts at: 0 299 621
Size: 299 322
BstZ17I GTA'TAC
Cuts at: 0 125 621
Size: 125 496
BtgI C'CryG_G
Cuts at: 0 188 621
Size: 188 433
EaeI y'GGCC_r
Cuts at: 0 87 191 621
Size: 87 104 430
Fragments arranged by size:
430 104 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4 621
Size: 4 617
EcoICRI GAG'CTC
Cuts at: 0 177 621
Size: 177 444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 491 621
Size: 280 211 130
Fragments arranged by size:
280 211 130
EcoO109I rG'GnC_Cy
Cuts at: 0 60 517 621
Size: 60 457 104
Fragments arranged by size:
457 104 60
HaeII r_GCGC'y
Cuts at: 0 231 554 621
Size: 231 323 67
Fragments arranged by size:
323 231 67
KpnI G_GTAC'C
Cuts at: 0 318 621
Size: 318 303
MscI TGG'CCA
Cuts at: 0 89 193 621
Size: 89 104 428
Fragments arranged by size:
428 104 89
MspA1I CmG'CkG
Cuts at: 0 45 201 212 330 487 574 580 621
Size: 45 156 11 118 157 87 6 41
Fragments arranged by size:
157 156 118 87 45 41 11 6
PstI C_TGCA'G
Cuts at: 0 44 621
Size: 44 577
PvuII CAG'CTG
Cuts at: 0 45 201 212 330 487 621
Size: 45 156 11 118 157 134
Fragments arranged by size:
157 156 134 118 45 11
SacI G_AGCT'C
Cuts at: 0 179 621
Size: 179 442
SfcI C'TryA_G
Cuts at: 0 40 621
Size: 40 581
SmaI CCC'GGG
Cuts at: 0 148 621
Size: 148 473
StyI C'CwwG_G
Cuts at: 0 608 621
Size: 608 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 511 552 621
Size: 511 41 69
Fragments arranged by size:
511 69 41
XmaI C'CCGG_G
Cuts at: 0 146 621
Size: 146 475
Enzymes that do cut:
AccI Acc65I AfeI AvaI BanI BanII BglI Bme1580I
BmrI BpmI BsaBI BsaHI BsaWI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BstXI BstYI BstZ17I
BtgI EaeI EciI EcoICRI Eco57MI EcoO109I HaeII KpnI
MscI MspA1I PstI PvuII SacI SfcI SmaI StyI
TaqII XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BsiEI
BsiWI BsmBI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI MfeI MluI MmeI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI