Restriction map for
pFN21AE1350
(Linear) (Six-base) MAPSORT of: KIEE1350.seq Check: 7904 from: 1 to: 3561
KIEE1350.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AatII G_ACGT'C
Cuts at: 0 2630 3561
Size: 2630 931
AccI GT'mk_AC
Cuts at: 0 511 3561
Size: 511 3050
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 3238 3526 3527 3561
Size: 103 3135 288 1 34
Fragments arranged by size:
3135 288 103 34 1
AfeI AGC'GCT
Cuts at: 0 2683 3561
Size: 2683 878
AflIII A'CryG_T
Cuts at: 0 1805 3561
Size: 1805 1756
AleI CACnn'nnGTG
Cuts at: 0 1153 3561
Size: 1153 2408
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 739 2602 3103 3561
Size: 739 1863 501 458
Fragments arranged by size:
1863 739 501 458
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3214 3246 3561
Size: 3214 32 315
Fragments arranged by size:
3214 315 32
AlwNI CAG_nnn'CTG
Cuts at: 0 907 961 2151 2544 3000 3561
Size: 907 54 1190 393 456 561
Fragments arranged by size:
1190 907 561 456 393 54
ApaI G_GGCC'C
Cuts at: 0 380 932 3561
Size: 380 552 2629
Fragments arranged by size:
2629 552 380
ApoI r'AATT_y
Cuts at: 0 3319 3561
Size: 3319 242
AvaI C'yCGr_G
Cuts at: 0 350 787 1226 1396 1700 2500 2823 3561
Size: 350 437 439 170 304 800 323 738
Fragments arranged by size:
800 738 439 437 350 323 304 170
BamHI G'GATC_C
Cuts at: 0 1349 2324 3561
Size: 1349 975 1237
Fragments arranged by size:
1349 1237 975
BanI G'GyrC_C
Cuts at: 0 798 1476 2248 2293 2401 3561
Size: 798 678 772 45 108 1160
Fragments arranged by size:
1160 798 772 678 108 45
BanII G_rGCy'C
Cuts at: 0 57 125 380 869 932 1701 2507 3310
Size: 57 68 255 489 63 769 806 803
Cuts at: 3310 3332 3561
Size: 22 229
Fragments arranged by size:
806 803 769 489 255 229 68 63
57 22
BbeI G_GCGC'C
Cuts at: 0 2297 2405 3561
Size: 2297 108 1156
Fragments arranged by size:
2297 1156 108
BbsI GAAGACnn'nnnn_
Cuts at: 0 628 702 3514 3561
Size: 628 74 2812 47
Fragments arranged by size:
2812 628 74 47
BbvCI CC'TCA_GC
Cuts at: 0 158 1612 1726 2164 3561
Size: 158 1454 114 438 1397
Fragments arranged by size:
1454 1397 438 158 114
BglI GCCn_nnn'nGGC
Cuts at: 0 2797 3561
Size: 2797 764
BglII A'GATC_T
Cuts at: 0 2417 2703 3017 3561
Size: 2417 286 314 544
Fragments arranged by size:
2417 544 314 286
BlpI GC'TnA_GC
Cuts at: 0 1213 1735 3561
Size: 1213 522 1826
Fragments arranged by size:
1826 1213 522
Bme1580I G_kGCm'C
Cuts at: 0 380 827 932 1795 2120 2251 2945 3146
Size: 380 447 105 863 325 131 694 201
Cuts at: 3146 3561
Size: 415
Fragments arranged by size:
863 694 447 415 380 325 201 131
105
BmgBI CAC'GTC
Cuts at: 0 489 3561
Size: 489 3072
BmrI ACTGGGnnnn_n'
Cuts at: 0 24 108 3561
Size: 24 84 3453
Fragments arranged by size:
3453 84 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 414 1936 3561
Size: 414 1522 1625
Fragments arranged by size:
1625 1522 414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1228 1272 1387 1443 1567 2194 2286
Size: 625 603 44 115 56 124 627 92
Cuts at: 2286 2434 3282 3561
Size: 148 848 279
Fragments arranged by size:
848 627 625 603 279 148 124 115
92 56 44
Bpu10I CC'TnA_GC
Cuts at: 0 158 236 1612 1726 2164 2371 3344 3561
Size: 158 78 1376 114 438 207 973 217
Fragments arranged by size:
1376 973 438 217 207 158 114 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2779 3346 3561
Size: 2779 567 215
Fragments arranged by size:
2779 567 215
BsaI GGTCTCn'nnnn_
Cuts at: 0 1998 3059 3561
Size: 1998 1061 502
Fragments arranged by size:
1998 1061 502
BsaAI yAC'GTr
Cuts at: 0 1155 2067 2940 3561
Size: 1155 912 873 621
Fragments arranged by size:
1155 912 873 621
BsaBI GATnn'nnATC
Cuts at: 0 1336 3561
Size: 1336 2225
BsaHI Gr'CG_yC
Cuts at: 0 2024 2294 2402 2627 2656 3466 3561
Size: 2024 270 108 225 29 810 95
Fragments arranged by size:
2024 810 270 225 108 95 29
BsaWI w'CCGG_w
Cuts at: 0 326 3561
Size: 326 3235
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1102 1132 3214 3244 3280 3310 3561
Size: 1102 30 2082 30 36 30 251
Fragments arranged by size:
2082 1102 251 36 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 191 532 535 622 1003 1006 1021 1864
Size: 191 341 3 87 381 3 15 843
Cuts at: 1864 2323 3561
Size: 459 1238
Fragments arranged by size:
1238 843 459 381 341 191 87 15
3 3
BseYI C'CCAG_C
Cuts at: 0 56 435 752 925 1463 1553 1570 1769
Size: 56 379 317 173 538 90 17 199
Cuts at: 1769 2144 2232 2813 2993 3011 3452 3561
Size: 375 88 581 180 18 441 109
Fragments arranged by size:
581 538 441 379 375 317 199 180
173 109 90 88 56 18 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 550 1399 3025 3561
Size: 79 471 849 1626 536
Fragments arranged by size:
1626 849 536 471 79
BsiEI CG_ry'CG
Cuts at: 0 515 1283 3561
Size: 515 768 2278
Fragments arranged by size:
2278 768 515
BsiHKAI G_wGCw'C
Cuts at: 0 1172 1429 1922 2075 2873 3332 3561
Size: 1172 257 493 153 798 459 229
Fragments arranged by size:
1172 798 493 459 257 229 153
BsmI GAATG_Cn'
Cuts at: 0 2350 3561
Size: 2350 1211
Bsp1286I G_dGCh'C
Cuts at: 0 57 125 380 827 869 932 1172 1429
Size: 57 68 255 447 42 63 240 257
Cuts at: 1429 1701 1795 1922 2075 2120 2251 2507 2873
Size: 272 94 127 153 45 131 256 366
Cuts at: 2873 2945 3146 3310 3332 3561
Size: 72 201 164 22 229
Fragments arranged by size:
447 366 272 257 256 255 240 229
201 164 153 131 127 94 72 68
63 57 45 42 22
BspEI T'CCGG_A
Cuts at: 0 326 3561
Size: 326 3235
BspHI T'CATG_A
Cuts at: 0 1982 3440 3561
Size: 1982 1458 121
Fragments arranged by size:
1982 1458 121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 707 2975 3561
Size: 707 2268 586
Fragments arranged by size:
2268 707 586
BsrBI CCG'CTC
Cuts at: 0 792 2106 2313 2822 3561
Size: 792 1314 207 509 739
Fragments arranged by size:
1314 792 739 509 207
BsrDI GCAATG_nn'
Cuts at: 0 176 3338 3561
Size: 176 3162 223
Fragments arranged by size:
3162 223 176
BsrFI r'CCGG_y
Cuts at: 0 357 659 1092 1187 1559 2492 2652 3561
Size: 357 302 433 95 372 933 160 909
Fragments arranged by size:
933 909 433 372 357 302 160 95
BssHII G'CGCG_C
Cuts at: 0 3076 3561
Size: 3076 485
BssSI C'ACGA_G
Cuts at: 0 940 1306 1879 2866 3154 3561
Size: 940 366 573 987 288 407
Fragments arranged by size:
987 940 573 407 366 288
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1497 2496 3561
Size: 1497 999 1065
Fragments arranged by size:
1497 1065 999
BstEII G'GTnAC_C
Cuts at: 0 3264 3561
Size: 3264 297
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2412 3561
Size: 2412 1149
BstYI r'GATC_y
Cuts at: 0 275 524 1349 2324 2417 2696 2703 3017
Size: 275 249 825 975 93 279 7 314
Cuts at: 3017 3561
Size: 544
Fragments arranged by size:
975 825 544 314 279 275 249 93
7
Bsu36I CC'TnA_GG
Cuts at: 0 838 1847 3561
Size: 838 1009 1714
Fragments arranged by size:
1714 1009 838
BtgI C'CryG_G
Cuts at: 0 62 71 591 1105 1321 1353 1896 2120
Size: 62 9 520 514 216 32 543 224
Cuts at: 2120 2381 3403 3561
Size: 261 1022 158
Fragments arranged by size:
1022 543 520 514 261 224 216 158
62 32 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 131 1935 3561
Size: 131 1804 1626
Fragments arranged by size:
1804 1626 131
BtsI GCAGTG_nn'
Cuts at: 0 1032 3561
Size: 1032 2529
DraIII CAC_nnn'GTG
Cuts at: 0 945 1158 1311 3561
Size: 945 213 153 2250
Fragments arranged by size:
2250 945 213 153
DrdI GACnn_nn'nnGTC
Cuts at: 0 3106 3465 3561
Size: 3106 359 96
Fragments arranged by size:
3106 359 96
EaeI y'GGCC_r
Cuts at: 0 23 614 979 1086 1280 2153 2208 2522
Size: 23 591 365 107 194 873 55 314
Cuts at: 2522 3561
Size: 1039
Fragments arranged by size:
1039 873 591 365 314 194 107 55
23
EagI C'GGCC_G
Cuts at: 0 1280 3561
Size: 1280 2281
EarI CTCTTCn'nnn_
Cuts at: 0 232 3010 3561
Size: 232 2778 551
Fragments arranged by size:
2778 551 232
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 577 646 1261 3561
Size: 577 69 615 2300
Fragments arranged by size:
2300 615 577 69
EcoICRI GAG'CTC
Cuts at: 0 3330 3561
Size: 3330 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 625 1228 1272 1387 1443 1567 2194
Size: 103 522 603 44 115 56 124 627
Cuts at: 2194 2286 2434 3238 3282 3526 3527 3561
Size: 92 148 804 44 244 1 34
Fragments arranged by size:
804 627 603 522 244 148 124 115
103 92 56 44 44 34 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 607 3133 3561
Size: 607 2526 428
Fragments arranged by size:
2526 607 428
EcoO109I rG'GnC_Cy
Cuts at: 0 377 929 1535 1842 1872 2436 3537 3561
Size: 377 552 606 307 30 564 1101 24
Fragments arranged by size:
1101 606 564 552 377 307 30 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 645 2751 3561
Size: 645 2106 810
Fragments arranged by size:
2106 810 645
FspI TGC'GCA
Cuts at: 0 2854 3561
Size: 2854 707
HaeII r_GCGC'y
Cuts at: 0 683 1240 2162 2297 2405 2685 3561
Size: 683 557 922 135 108 280 876
Fragments arranged by size:
922 876 683 557 280 135 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 424 456 478 510 1618 1650 2439 2471
Size: 424 32 22 32 1108 32 789 32
Cuts at: 2471 2688 2720 3238 3270 3561
Size: 217 32 518 32 291
Fragments arranged by size:
1108 789 518 424 291 217 32 32
32 32 32 32 22
HincII GTy'rAC
Cuts at: 0 512 1752 3561
Size: 512 1240 1809
Fragments arranged by size:
1809 1240 512
KasI G'GCGC_C
Cuts at: 0 2293 2401 3561
Size: 2293 108 1160
Fragments arranged by size:
2293 1160 108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2639 2776 3561
Size: 2639 137 785
Fragments arranged by size:
2639 785 137
MscI TGG'CCA
Cuts at: 0 25 616 981 2155 2524 3561
Size: 25 591 365 1174 369 1037
Fragments arranged by size:
1174 1037 591 369 365 25
MslI CAynn'nnrTG
Cuts at: 0 498 1153 1687 2198 2380 3561
Size: 498 655 534 511 182 1181
Fragments arranged by size:
1181 655 534 511 498 182
MspA1I CmG'CkG
Cuts at: 0 157 1107 1773 1838 2151 2520 2925 2997
Size: 157 950 666 65 313 369 405 72
Cuts at: 2997 3561
Size: 564
Fragments arranged by size:
950 666 564 405 369 313 157 72
65
NaeI GCC'GGC
Cuts at: 0 661 1094 3561
Size: 661 433 2467
Fragments arranged by size:
2467 661 433
NarI GG'CG_CC
Cuts at: 0 2294 2402 3561
Size: 2294 108 1159
Fragments arranged by size:
2294 1159 108
NcoI C'CATG_G
Cuts at: 0 62 71 591 2120 2381 3561
Size: 62 9 520 1529 261 1180
Fragments arranged by size:
1529 1180 520 261 62 9
NgoMIV G'CCGG_C
Cuts at: 0 659 1092 3561
Size: 659 433 2469
Fragments arranged by size:
2469 659 433
NspI r_CATG'y
Cuts at: 0 1491 3561
Size: 1491 2070
PasI CC'CwG_GG
Cuts at: 0 125 2946 3561
Size: 125 2821 615
Fragments arranged by size:
2821 615 125
PflMI CCAn_nnn'nTGG
Cuts at: 0 63 759 1311 2126 2151 3561
Size: 63 696 552 815 25 1410
Fragments arranged by size:
1410 815 696 552 63 25
PfoI T'CCnGG_A
Cuts at: 0 428 878 1139 1340 2411 3561
Size: 428 450 261 201 1071 1150
Fragments arranged by size:
1150 1071 450 428 261 201
PmlI CAC'GTG
Cuts at: 0 1155 3561
Size: 1155 2406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2632 2664 3214 3246 3561
Size: 2632 32 550 32 315
Fragments arranged by size:
2632 550 315 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1842 1872 3561
Size: 1842 30 1689
Fragments arranged by size:
1842 1689 30
PshAI GACnn'nnGTC
Cuts at: 0 1937 3416 3561
Size: 1937 1479 145
Fragments arranged by size:
1937 1479 145
PspOMI G'GGCC_C
Cuts at: 0 376 928 3561
Size: 376 552 2633
Fragments arranged by size:
2633 552 376
PspXI vC'TCGA_Gb
Cuts at: 0 787 3561
Size: 787 2774
PstI C_TGCA'G
Cuts at: 0 2447 2609 2924 3561
Size: 2447 162 315 637
Fragments arranged by size:
2447 637 315 162
PvuII CAG'CTG
Cuts at: 0 157 1773 2151 2520 2925 2997 3561
Size: 157 1616 378 369 405 72 564
Fragments arranged by size:
1616 564 405 378 369 157 72
SacI G_AGCT'C
Cuts at: 0 3332 3561
Size: 3332 229
SacII CC_GC'GG
Cuts at: 0 1108 3561
Size: 1108 2453
SalI G'TCGA_C
Cuts at: 0 510 3561
Size: 510 3051
SanDI GG'GwC_CC
Cuts at: 0 1842 3561
Size: 1842 1719
SapI GCTCTTCn'nnn_
Cuts at: 0 3010 3561
Size: 3010 551
ScaI AGT'ACT
Cuts at: 0 3160 3561
Size: 3160 401
SexAI A'CCwGG_T
Cuts at: 0 2186 2352 3561
Size: 2186 166 1209
Fragments arranged by size:
2186 1209 166
SfcI C'TryA_G
Cuts at: 0 899 2443 2605 2920 3561
Size: 899 1544 162 315 641
Fragments arranged by size:
1544 899 641 315 162
SfoI GGC'GCC
Cuts at: 0 2295 2403 3561
Size: 2295 108 1158
Fragments arranged by size:
2295 1158 108
SgrAI Cr'CCGG_yG
Cuts at: 0 2652 3561
Size: 2652 909
SmaI CCC'GGG
Cuts at: 0 1228 3561
Size: 1228 2333
SmlI C'TyrA_G
Cuts at: 0 787 2794 3325 3561
Size: 787 2007 531 236
Fragments arranged by size:
2007 787 531 236
SphI G_CATG'C
Cuts at: 0 1491 3561
Size: 1491 2070
StuI AGG'CCT
Cuts at: 0 80 3561
Size: 80 3481
StyI C'CwwG_G
Cuts at: 0 62 71 591 1067 2120 2381 2904 3134
Size: 62 9 520 476 1053 261 523 230
Cuts at: 3134 3528 3561
Size: 394 33
Fragments arranged by size:
1053 523 520 476 394 261 230 62
33 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 529 1165 1783 3561
Size: 529 636 618 1778
Fragments arranged by size:
1778 636 618 529
TatI w'GTAC_w
Cuts at: 0 2738 2882 3158 3561
Size: 2738 144 276 403
Fragments arranged by size:
2738 403 276 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1102 1134 1186 1218 3280 3312 3561
Size: 1102 32 52 32 2062 32 249
Fragments arranged by size:
2062 1102 249 52 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 3561
Size: 69 3492
XhoI C'TCGA_G
Cuts at: 0 787 3561
Size: 787 2774
XmaI C'CCGG_G
Cuts at: 0 1226 3561
Size: 1226 2335
XmnI GAAnn'nnTTC
Cuts at: 0 709 3561
Size: 709 2852
ZraI GAC'GTC
Cuts at: 0 2628 3561
Size: 2628 933
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuII SacI SacII SalI
SanDI SapI ScaI SexAI SfcI SfoI SgrAI SmaI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AhdI ApaLI AscI
AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI
BmtI BsiWI BsmBI BsrGI BstBI BstZ17I ClaI CspCI
DraI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PsiI PsrI PvuI RsrII SbfI SfiI
SgfI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI