(Linear) (Six-base) MAPSORT of: KIEE1348.seq  Check: 2420  from: 1  to: 1416



KIEE1348.orf

 With 182 enzymes: SgfI * 

                        December 17, 2009 16:58  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1234    1416 
   Size:       1234     182
 
AflIII A'CryG_T

Cuts at:      0    1283    1416 
   Size:       1283     133
 
AlwNI CAG_nnn'CTG

Cuts at:      0     394    1416 
   Size:        394    1022
 
ApoI r'AATT_y

Cuts at:      0    1193    1416 
   Size:       1193     223
 
AseI AT'TA_AT

Cuts at:      0     737    1416 
   Size:        737     679
 
BamHI G'GATC_C

Cuts at:      0     549    1416 
   Size:        549     867
 
BanI G'GyrC_C

Cuts at:      0     355    1416 
   Size:        355    1061
 
BanII G_rGCy'C

Cuts at:      0      26    1416 
   Size:         26    1390
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     112    1416 
   Size:        112    1304
 
BbvCI CC'TCA_GC

Cuts at:      0     320    1416 
   Size:        320    1096
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1382    1416 
   Size:       1382      34
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1265    1416 
   Size:       1265     151
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     890    1416 
   Size:        890     526
 
Bpu10I CC'TnA_GC

Cuts at:      0     320    1416 
   Size:        320    1096
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      83    1002    1416 
   Size:         83     919     414

  Fragments arranged by size: 

                 919     414      83
 
BsaAI yAC'GTr

Cuts at:      0     275    1416 
   Size:        275    1141
 
BsaWI w'CCGG_w

Cuts at:      0      11    1416 
   Size:         11    1405
 
BseYI C'CCAG_C

Cuts at:      0      31    1416 
   Size:         31    1385
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     921    1416 
   Size:        921     495
 
Bsp1286I G_dGCh'C

Cuts at:      0      26    1416 
   Size:         26    1390
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     101     806    1152    1416 
   Size:        101     705     346     264

  Fragments arranged by size: 

                 705     346     264     101
 
BsrGI T'GTAC_A

Cuts at:      0     964    1016    1416 
   Size:        964      52     400

  Fragments arranged by size: 

                 964     400      52
 
BstBI TT'CG_AA

Cuts at:      0     365    1416 
   Size:        365    1051
 
BstYI r'GATC_y

Cuts at:      0     197     549    1416 
   Size:        197     352     867

  Fragments arranged by size: 

                 867     352     197
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      12    1416 
   Size:         12    1404
 
BtsI GCAGTG_nn'

Cuts at:      0     723    1416 
   Size:        723     693
 
DraI TTT'AAA

Cuts at:      0     153    1416 
   Size:        153    1263
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     331    1416 
   Size:        331    1085
 
EarI CTCTTCn'nnn_

Cuts at:      0    1210    1416 
   Size:       1210     206
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     890    1234    1416 
   Size:        890     344     182

  Fragments arranged by size: 

                 890     344     182
 
EcoO109I rG'GnC_Cy

Cuts at:      0     265    1416 
   Size:        265    1151
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     284    1416 
   Size:        284    1132
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     739     771    1416 
   Size:        739      32     645

  Fragments arranged by size: 

                 739     645      32
 
HincII GTy'rAC

Cuts at:      0    1167    1416 
   Size:       1167     249
 
MfeI C'AATT_G

Cuts at:      0     143    1416 
   Size:        143    1273
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     363     866    1416 
   Size:         66     297     503     550

  Fragments arranged by size: 

                 550     503     297      66
 
MspA1I CmG'CkG

Cuts at:      0     430    1416 
   Size:        430     986
 
NdeI CA'TA_TG

Cuts at:      0     972    1416 
   Size:        972     444
 
NspI r_CATG'y

Cuts at:      0    1287    1397    1416 
   Size:       1287     110      19

  Fragments arranged by size: 

                1287     110      19
 
PciI A'CATG_T

Cuts at:      0    1283    1416 
   Size:       1283     133
 
PpuMI rG'GwC_Cy

Cuts at:      0     265    1416 
   Size:        265    1151
 
PsiI TTA'TAA

Cuts at:      0     635    1416 
   Size:        635     781
 
PstI C_TGCA'G

Cuts at:      0     347     624    1163    1416 
   Size:        347     277     539     253

  Fragments arranged by size: 

                 539     347     277     253
 
PvuII CAG'CTG

Cuts at:      0     430    1416 
   Size:        430     986
 
SfcI C'TryA_G

Cuts at:      0     343     543     620    1159    1416 
   Size:        343     200      77     539     257

  Fragments arranged by size: 

                 539     343     257     200      77
 
SnaBI TAC'GTA

Cuts at:      0     275    1416 
   Size:        275    1141
 
SphI G_CATG'C

Cuts at:      0    1397    1416 
   Size:       1397      19
 
StuI AGG'CCT

Cuts at:      0     895    1416 
   Size:        895     521
 
StyI C'CwwG_G

Cuts at:      0     296     890    1320    1416 
   Size:        296     594     430      96

  Fragments arranged by size: 

                 594     430     296      96
 
TatI w'GTAC_w

Cuts at:      0     964    1016    1416 
   Size:        964      52     400

  Fragments arranged by size: 

                 964     400      52
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     851     883    1251    1283    1416 
   Size:        851      32     368      32     133

  Fragments arranged by size: 

                 851     368     133      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1193    1416 
   Size:       1193     223
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     AseI    BamHI     BanI    BanII
     BbsI    BbvCI     BcgI     BmrI     BpmI   Bpu10I     BsaI    BsaAI
    BsaWI    BseYI     BsgI Bsp1286I    BspMI    BsrGI    BstBI    BstYI
    BtgZI     BtsI     DraI     DrdI     EarI  Eco57MI EcoO109I     FalI
    Hin4I   HincII     MfeI     MmeI   MspA1I     NdeI     NspI     PciI
    PpuMI     PsiI     PstI    PvuII     SfcI    SnaBI     SphI     StuI
     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI     BbeI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI    BsaBI
    BsaHI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MluI
     MscI     MslI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI     SrfI     SspI
     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI