Restriction map for
pFN21AE1347
(Linear) (Six-base) MAPSORT of: KIEE1347.seq Check: 5236 from: 1 to: 1137
KIEE1347.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1137
Size: 229 908
AlwNI CAG_nnn'CTG
Cuts at: 0 69 401 1137
Size: 69 332 736
Fragments arranged by size:
736 332 69
ApaLI G'TGCA_C
Cuts at: 0 440 497 1137
Size: 440 57 640
Fragments arranged by size:
640 440 57
ApoI r'AATT_y
Cuts at: 0 238 324 1137
Size: 238 86 813
Fragments arranged by size:
813 238 86
AvaI C'yCGr_G
Cuts at: 0 332 1137
Size: 332 805
AvrII C'CTAG_G
Cuts at: 0 757 1137
Size: 757 380
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 539 572 1137
Size: 539 33 565
Fragments arranged by size:
565 539 33
BanII G_rGCy'C
Cuts at: 0 837 1137
Size: 837 300
BbsI GAAGACnn'nnnn_
Cuts at: 0 1028 1137
Size: 1028 109
BciVI GTATCCnnnnn_n'
Cuts at: 0 1104 1137
Size: 1104 33
BclI T'GATC_A
Cuts at: 0 392 896 1137
Size: 392 504 241
Fragments arranged by size:
504 392 241
BglII A'GATC_T
Cuts at: 0 885 1083 1137
Size: 885 198 54
Fragments arranged by size:
885 198 54
Bme1580I G_kGCm'C
Cuts at: 0 444 501 1137
Size: 444 57 636
Fragments arranged by size:
636 444 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 440 1137
Size: 440 697
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 953 1137
Size: 91 862 184
Fragments arranged by size:
862 184 91
Bpu10I CC'TnA_GC
Cuts at: 0 722 1137
Size: 722 415
BsaI GGTCTCn'nnnn_
Cuts at: 0 313 1137
Size: 313 824
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 151 1137
Size: 46 105 986
Fragments arranged by size:
986 105 46
BseYI C'CCAG_C
Cuts at: 0 1097 1137
Size: 1097 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1137
Size: 82 1055
BsiEI CG_ry'CG
Cuts at: 0 17 213 1137
Size: 17 196 924
Fragments arranged by size:
924 196 17
BsiHKAI G_wGCw'C
Cuts at: 0 444 501 1137
Size: 444 57 636
Fragments arranged by size:
636 444 57
Bsp1286I G_dGCh'C
Cuts at: 0 444 501 837 1137
Size: 444 57 336 300
Fragments arranged by size:
444 336 300 57
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 1137
Size: 62 1075
BsrGI T'GTAC_A
Cuts at: 0 459 1137
Size: 459 678
BstYI r'GATC_y
Cuts at: 0 619 885 1083 1137
Size: 619 266 198 54
Fragments arranged by size:
619 266 198 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 961 1137
Size: 961 176
BtsI GCAGTG_nn'
Cuts at: 0 26 298 1137
Size: 26 272 839
Fragments arranged by size:
839 272 26
DraI TTT'AAA
Cuts at: 0 197 845 1137
Size: 197 648 292
Fragments arranged by size:
648 292 197
EaeI y'GGCC_r
Cuts at: 0 14 26 1137
Size: 14 12 1111
Fragments arranged by size:
1111 14 12
EagI C'GGCC_G
Cuts at: 0 14 1137
Size: 14 1123
EarI CTCTTCn'nnn_
Cuts at: 0 1004 1137
Size: 1004 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 229 953 1137
Size: 91 138 724 184
Fragments arranged by size:
724 184 138 91
EcoO109I rG'GnC_Cy
Cuts at: 0 51 84 1137
Size: 51 33 1053
Fragments arranged by size:
1053 51 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 460 682 1042 1137
Size: 460 222 360 95
Fragments arranged by size:
460 360 222 95
HaeII r_GCGC'y
Cuts at: 0 402 1137
Size: 402 735
HincII GTy'rAC
Cuts at: 0 406 1137
Size: 406 731
HindIII A'AGCT_T
Cuts at: 0 1063 1137
Size: 1063 74
MspA1I CmG'CkG
Cuts at: 0 80 118 1137
Size: 80 38 1019
Fragments arranged by size:
1019 80 38
NotI GC'GGCC_GC
Cuts at: 0 14 1137
Size: 14 1123
PflMI CCAn_nnn'nTGG
Cuts at: 0 430 1137
Size: 430 707
PpuMI rG'GwC_Cy
Cuts at: 0 51 84 1137
Size: 51 33 1053
Fragments arranged by size:
1053 51 33
PvuII CAG'CTG
Cuts at: 0 118 1137
Size: 118 1019
StyI C'CwwG_G
Cuts at: 0 757 1137
Size: 757 380
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 358 1137
Size: 358 779
TatI w'GTAC_w
Cuts at: 0 459 1137
Size: 459 678
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 583 615 1137
Size: 583 32 522
Fragments arranged by size:
583 522 32
XmnI GAAnn'nnTTC
Cuts at: 0 242 1137
Size: 242 895
Enzymes that do cut:
AcuI AlwNI ApaLI ApoI AvaI AvrII BaeI BanII
BbsI BciVI BclI BglII Bme1580I BmrI BpmI Bpu10I
BsaI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrGI BstYI BtgZI BtsI DraI EaeI EagI EarI
Eco57MI EcoO109I FalI HaeII HincII HindIII MspA1I NotI
PflMI PpuMI PvuII StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AseI
AsiSI BamHI BanI BbeI BbvCI BcgI BfrBI BglI
BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI
BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII DrdI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI ZraI