Restriction map for
pFN21AE1344
(Linear) (Six-base) MAPSORT of: KIEE1344.seq Check: 5974 from: 1 to: 1680
KIEE1344.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1479 1680
Size: 1479 201
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 439 1360 1680
Size: 350 89 921 320
Fragments arranged by size:
921 350 320 89
AflII C'TTAA_G
Cuts at: 0 271 633 1680
Size: 271 362 1047
Fragments arranged by size:
1047 362 271
AleI CACnn'nnGTG
Cuts at: 0 569 1680
Size: 569 1111
AlwNI CAG_nnn'CTG
Cuts at: 0 1101 1680
Size: 1101 579
ApaI G_GGCC'C
Cuts at: 0 829 1680
Size: 829 851
ApoI r'AATT_y
Cuts at: 0 396 549 974 1680
Size: 396 153 425 706
Fragments arranged by size:
706 425 396 153
AvaI C'yCGr_G
Cuts at: 0 59 1680
Size: 59 1621
AvrII C'CTAG_G
Cuts at: 0 982 1680
Size: 982 698
BanII G_rGCy'C
Cuts at: 0 829 1535 1680
Size: 829 706 145
Fragments arranged by size:
829 706 145
BciVI GTATCCnnnnn_n'
Cuts at: 0 774 1680
Size: 774 906
BclI T'GATC_A
Cuts at: 0 1412 1680
Size: 1412 268
BglII A'GATC_T
Cuts at: 0 1385 1680
Size: 1385 295
BlpI GC'TnA_GC
Cuts at: 0 916 1680
Size: 916 764
Bme1580I G_kGCm'C
Cuts at: 0 829 1131 1680
Size: 829 302 549
Fragments arranged by size:
829 549 302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 1680
Size: 69 1611
Bpu10I CC'TnA_GC
Cuts at: 0 740 1680
Size: 740 940
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 640 726 1362 1680
Size: 576 64 86 636 318
Fragments arranged by size:
636 576 318 86 64
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 117 147 1680
Size: 117 30 1533
Fragments arranged by size:
1533 117 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 125 1680
Size: 125 1555
BseYI C'CCAG_C
Cuts at: 0 729 775 1680
Size: 729 46 905
Fragments arranged by size:
905 729 46
BsiHKAI G_wGCw'C
Cuts at: 0 609 1359 1680
Size: 609 750 321
Fragments arranged by size:
750 609 321
Bsp1286I G_dGCh'C
Cuts at: 0 609 829 1131 1359 1535 1680
Size: 609 220 302 228 176 145
Fragments arranged by size:
609 302 228 220 176 145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1479 1680
Size: 1479 201
BsrGI T'GTAC_A
Cuts at: 0 890 1680
Size: 890 790
BssSI C'ACGA_G
Cuts at: 0 137 1680
Size: 137 1543
BstYI r'GATC_y
Cuts at: 0 960 1385 1680
Size: 960 425 295
Fragments arranged by size:
960 425 295
BtgI C'CryG_G
Cuts at: 0 910 1680
Size: 910 770
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 194 1680
Size: 194 1486
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1001 1036 1680
Size: 1001 35 644
Fragments arranged by size:
1001 644 35
DraI TTT'AAA
Cuts at: 0 678 1680
Size: 678 1002
DraIII CAC_nnn'GTG
Cuts at: 0 933 1680
Size: 933 747
DrdI GACnn_nn'nnGTC
Cuts at: 0 312 1680
Size: 312 1368
EaeI y'GGCC_r
Cuts at: 0 907 1680
Size: 907 773
EarI CTCTTCn'nnn_
Cuts at: 0 752 1379 1604 1680
Size: 752 627 225 76
Fragments arranged by size:
752 627 225 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 350 439 1360 1680
Size: 69 281 89 921 320
Fragments arranged by size:
921 320 281 89 69
EcoNI CCTnn'n_nnAGG
Cuts at: 0 658 1680
Size: 658 1022
EcoO109I rG'GnC_Cy
Cuts at: 0 825 826 1680
Size: 825 1 854
Fragments arranged by size:
854 825 1
EcoRI G'AATT_C
Cuts at: 0 974 1680
Size: 974 706
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1090 1680
Size: 1090 590
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1272 1304 1504 1536 1680
Size: 1272 32 200 32 144
Fragments arranged by size:
1272 200 144 32 32
HindIII A'AGCT_T
Cuts at: 0 1643 1680
Size: 1643 37
MscI TGG'CCA
Cuts at: 0 909 1680
Size: 909 771
MslI CAynn'nnrTG
Cuts at: 0 569 835 1680
Size: 569 266 845
Fragments arranged by size:
845 569 266
MspA1I CmG'CkG
Cuts at: 0 303 499 775 1017 1680
Size: 303 196 276 242 663
Fragments arranged by size:
663 303 276 242 196
NcoI C'CATG_G
Cuts at: 0 910 1680
Size: 910 770
NdeI CA'TA_TG
Cuts at: 0 809 1680
Size: 809 871
NspI r_CATG'y
Cuts at: 0 437 702 1630 1680
Size: 437 265 928 50
Fragments arranged by size:
928 437 265 50
PfoI T'CCnGG_A
Cuts at: 0 609 1680
Size: 609 1071
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1082 1114 1680
Size: 1082 32 566
Fragments arranged by size:
1082 566 32
PspOMI G'GGCC_C
Cuts at: 0 825 1680
Size: 825 855
PstI C_TGCA'G
Cuts at: 0 819 1116 1680
Size: 819 297 564
Fragments arranged by size:
819 564 297
PvuII CAG'CTG
Cuts at: 0 303 499 775 1017 1680
Size: 303 196 276 242 663
Fragments arranged by size:
663 303 276 242 196
SfcI C'TryA_G
Cuts at: 0 815 1112 1464 1680
Size: 815 297 352 216
Fragments arranged by size:
815 352 297 216
SmlI C'TyrA_G
Cuts at: 0 271 555 633 655 705 1341 1680
Size: 271 284 78 22 50 636 339
Fragments arranged by size:
636 339 284 271 78 50 22
StyI C'CwwG_G
Cuts at: 0 910 982 1680
Size: 910 72 698
Fragments arranged by size:
910 698 72
TatI w'GTAC_w
Cuts at: 0 890 1680
Size: 890 790
XbaI T'CTAG_A
Cuts at: 0 1087 1680
Size: 1087 593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 171 837 1680
Size: 171 666 843
Fragments arranged by size:
843 666 171
Enzymes that do cut:
AarI AcuI AflII AleI AlwNI ApaI ApoI AvaI
AvrII BanII BciVI BclI BglII BlpI Bme1580I BpmI
Bpu10I BpuEI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrGI BssSI BstYI BtgI BtgZI CspCI DraI DraIII
DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I HindIII MscI MslI MspA1I NcoI NdeI NspI
PfoI PpiI PspOMI PstI PvuII SfcI SmlI StyI
TatI XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI BaeI BamHI
BanI BbeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmrI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI EagI EciI EcoICRI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XhoI XmaI XmnI
ZraI