(Linear) (Six-base) MAPSORT of: KIEE1342.seq  Check: 2770  from: 1  to: 561



KIEE1342.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:36  ..

AccI GT'mk_AC

Cuts at:      0     329     561 
   Size:        329     232
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     561 
   Size:        110     451
 
AflIII A'CryG_T

Cuts at:      0     437     561 
   Size:        437     124
 
AlwNI CAG_nnn'CTG

Cuts at:      0     147     561 
   Size:        147     414
 
ApoI r'AATT_y

Cuts at:      0      91     535     561 
   Size:         91     444      26

  Fragments arranged by size: 

                 444      91      26
 
AseI AT'TA_AT

Cuts at:      0     215     561 
   Size:        215     346
 
BanII G_rGCy'C

Cuts at:      0     261     561 
   Size:        261     300
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     172     561 
   Size:        172     389
 
Bpu10I CC'TnA_GC

Cuts at:      0      82     561 
   Size:         82     479
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     561 
   Size:        223     338
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     529     561 
   Size:        529      32
 
BsiHKAI G_wGCw'C

Cuts at:      0     261     561 
   Size:        261     300
 
Bsp1286I G_dGCh'C

Cuts at:      0     261     561 
   Size:        261     300
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      74     561 
   Size:         74     487
 
BsrBI CCG'CTC

Cuts at:      0      81     561 
   Size:         81     480
 
DraI TTT'AAA

Cuts at:      0     203     561 
   Size:        203     358
 
EaeI y'GGCC_r

Cuts at:      0      73     561 
   Size:         73     488
 
EarI CTCTTCn'nnn_

Cuts at:      0     129     561 
   Size:        129     432
 
EcoICRI GAG'CTC

Cuts at:      0     259     561 
   Size:        259     302
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     561 
   Size:        110     451
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     251     561 
   Size:        251     310
 
EcoO109I rG'GnC_Cy

Cuts at:      0      63     561 
   Size:         63     498
 
EcoRI G'AATT_C

Cuts at:      0      91     561 
   Size:         91     470
 
MscI TGG'CCA

Cuts at:      0      75     561 
   Size:         75     486
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      42     561 
   Size:         42     519
 
PfoI T'CCnGG_A

Cuts at:      0     509     561 
   Size:        509      52
 
PpuMI rG'GwC_Cy

Cuts at:      0      63     561 
   Size:         63     498
 
PsiI TTA'TAA

Cuts at:      0     365     561 
   Size:        365     196
 
SacI G_AGCT'C

Cuts at:      0     261     561 
   Size:        261     300
 
SexAI A'CCwGG_T

Cuts at:      0     169     561 
   Size:        169     392
 
SmlI C'TyrA_G

Cuts at:      0     238     561 
   Size:        238     323
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     398     561 
   Size:        398     163
 
TatI w'GTAC_w

Cuts at:      0     530     561 
   Size:        530      31
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     333     365     429     461     561 
   Size:        333      32      64      32     100

  Fragments arranged by size: 

                 333     100      64      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     334     561 
   Size:        334     227
 
XbaI T'CTAG_A

Cuts at:      0     279     561 
   Size:        279     282
 
XmnI GAAnn'nnTTC

Cuts at:      0      91     561 
   Size:         91     470
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApoI     AseI    BanII     BbsI
   Bpu10I    BpuEI    BseRI  BsiHKAI Bsp1286I    BspMI    BsrBI     DraI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     MscI
    PflMI     PfoI    PpuMI     PsiI     SacI    SexAI     SmlI    TaqII
     TatI     TstI  Tth111I     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     XcmI     XhoI     XmaI     ZraI