(Linear) (Six-base) MAPSORT of: KIEE1341.seq  Check: 2433  from: 1  to: 498



KIEE1341.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:36  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     361     498 
   Size:        344      17     137

  Fragments arranged by size: 

                 344     137      17
 
AflII C'TTAA_G

Cuts at:      0     268     498 
   Size:        268     230
 
AflIII A'CryG_T

Cuts at:      0      47     498 
   Size:         47     451
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     352     498 
   Size:        352     146
 
ApaLI G'TGCA_C

Cuts at:      0     466     498 
   Size:        466      32
 
ApoI r'AATT_y

Cuts at:      0      82     498 
   Size:         82     416
 
BanI G'GyrC_C

Cuts at:      0       3      34     498 
   Size:          3      31     464

  Fragments arranged by size: 

                 464      31       3
 
BbeI G_GCGC'C

Cuts at:      0      38     498 
   Size:         38     460
 
BclI T'GATC_A

Cuts at:      0     206     498 
   Size:        206     292
 
Bme1580I G_kGCm'C

Cuts at:      0     470     498 
   Size:        470      28
 
BmgBI CAC'GTC

Cuts at:      0     435     498 
   Size:        435      63
 
BsaHI Gr'CG_yC

Cuts at:      0      35     498 
   Size:         35     463
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      37      67     498 
   Size:         37      30     431

  Fragments arranged by size: 

                 431      37      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     412     498 
   Size:        412      86
 
BsiHKAI G_wGCw'C

Cuts at:      0     470     498 
   Size:        470      28
 
BsmI GAATG_Cn'

Cuts at:      0     304     498 
   Size:        304     194
 
Bsp1286I G_dGCh'C

Cuts at:      0     470     498 
   Size:        470      28
 
BsrBI CCG'CTC

Cuts at:      0     277     498 
   Size:        277     221
 
BstBI TT'CG_AA

Cuts at:      0      62     498 
   Size:         62     436
 
Bsu36I CC'TnA_GG

Cuts at:      0     284     498 
   Size:        284     214
 
BtgI C'CryG_G

Cuts at:      0     480     498 
   Size:        480      18
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     482     498 
   Size:         16     466      16

  Fragments arranged by size: 

                 466      16      16
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     394     498 
   Size:        394     104
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     361     498 
   Size:        344      17     137

  Fragments arranged by size: 

                 344     137      17
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     111     498 
   Size:        111     387
 
HaeII r_GCGC'y

Cuts at:      0      38     498 
   Size:         38     460
 
HincII GTy'rAC

Cuts at:      0     405     498 
   Size:        405      93
 
KasI G'GCGC_C

Cuts at:      0      34     498 
   Size:         34     464
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     498 
   Size:         68     430
 
MslI CAynn'nnrTG

Cuts at:      0     103     498 
   Size:        103     395
 
NarI GG'CG_CC

Cuts at:      0      35     498 
   Size:         35     463
 
PfoI T'CCnGG_A

Cuts at:      0      77     498 
   Size:         77     421
 
SexAI A'CCwGG_T

Cuts at:      0     461     498 
   Size:        461      37
 
SfoI GGC'GCC

Cuts at:      0      36     498 
   Size:         36     462
 
SmlI C'TyrA_G

Cuts at:      0     268     498 
   Size:        268     230
 
StuI AGG'CCT

Cuts at:      0      94     498 
   Size:         94     404
 
StyI C'CwwG_G

Cuts at:      0     165     392     498 
   Size:        165     227     106

  Fragments arranged by size: 

                 227     165     106
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     493     498 
   Size:        493       5
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     118     498 
   Size:        118     380
 
XmnI GAAnn'nnTTC

Cuts at:      0     293     498 
   Size:        293     205
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AlfI    ApaLI     ApoI     BanI     BbeI
     BclI Bme1580I    BmgBI    BsaHI    BsaXI    BseRI  BsiHKAI     BsmI
 Bsp1286I    BsrBI    BstBI   Bsu36I     BtgI     EarI     EciI  Eco57MI
     FalI    HaeII   HincII     KasI     MmeI     MslI     NarI     PfoI
    SexAI     SfoI     SmlI     StuI     StyI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BseYI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI