Restriction map for
pFN21AE1340
(Linear) (Six-base) MAPSORT of: KIEE1340.seq Check: 8282 from: 1 to: 2406
KIEE1340.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 231 676 1003 2329 2406
Size: 231 445 327 1326 77
Fragments arranged by size:
1326 445 327 231 77
AccI GT'mk_AC
Cuts at: 0 1099 2406
Size: 1099 1307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 2328 2406
Size: 871 1457 78
Fragments arranged by size:
1457 871 78
AflII C'TTAA_G
Cuts at: 0 553 2406
Size: 553 1853
AflIII A'CryG_T
Cuts at: 0 965 1118 2012 2406
Size: 965 153 894 394
Fragments arranged by size:
965 894 394 153
AhdI GACnn_n'nnGTC
Cuts at: 0 390 2016 2406
Size: 390 1626 390
Fragments arranged by size:
1626 390 390
AleI CACnn'nnGTG
Cuts at: 0 781 2006 2406
Size: 781 1225 400
Fragments arranged by size:
1225 781 400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 619 2406
Size: 619 1787
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2077 2109 2406
Size: 2077 32 297
Fragments arranged by size:
2077 297 32
AlwNI CAG_nnn'CTG
Cuts at: 0 132 487 849 1806 2321 2406
Size: 132 355 362 957 515 85
Fragments arranged by size:
957 515 362 355 132 85
ApaI G_GGCC'C
Cuts at: 0 1634 1692 2406
Size: 1634 58 714
Fragments arranged by size:
1634 714 58
AvaI C'yCGr_G
Cuts at: 0 1087 1388 1790 1972 2053 2406
Size: 1087 301 402 182 81 353
Fragments arranged by size:
1087 402 353 301 182 81
AvrII C'CTAG_G
Cuts at: 0 1416 2406
Size: 1416 990
BamHI G'GATC_C
Cuts at: 0 2027 2368 2406
Size: 2027 341 38
Fragments arranged by size:
2027 341 38
BanI G'GyrC_C
Cuts at: 0 219 642 1193 1694 1968 2406
Size: 219 423 551 501 274 438
Fragments arranged by size:
551 501 438 423 274 219
BanII G_rGCy'C
Cuts at: 0 95 764 1129 1634 1692 2062 2406
Size: 95 669 365 505 58 370 344
Fragments arranged by size:
669 505 370 365 344 95 58
BbeI G_GCGC'C
Cuts at: 0 1698 1972 2406
Size: 1698 274 434
Fragments arranged by size:
1698 434 274
BbsI GAAGACnn'nnnn_
Cuts at: 0 2334 2406
Size: 2334 72
BbvCI CC'TCA_GC
Cuts at: 0 1714 2406
Size: 1714 692
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 861 895 996 1030 2406
Size: 861 34 101 34 1376
Fragments arranged by size:
1376 861 101 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1834 2078 2406
Size: 1834 244 328
Fragments arranged by size:
1834 328 244
BglI GCCn_nnn'nGGC
Cuts at: 0 1004 1703 2406
Size: 1004 699 703
Fragments arranged by size:
1004 703 699
BglII A'GATC_T
Cuts at: 0 1356 2042 2406
Size: 1356 686 364
Fragments arranged by size:
1356 686 364
BlpI GC'TnA_GC
Cuts at: 0 179 2406
Size: 179 2227
Bme1580I G_kGCm'C
Cuts at: 0 647 1634 1692 2406
Size: 647 987 58 714
Fragments arranged by size:
987 714 647 58
BmgBI CAC'GTC
Cuts at: 0 798 2013 2406
Size: 798 1215 393
Fragments arranged by size:
1215 798 393
BmrI ACTGGGnnnn_n'
Cuts at: 0 1753 2406
Size: 1753 653
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1284 2112 2406
Size: 1284 828 294
Fragments arranged by size:
1284 828 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 85 133 264 351 639 745 1279
Size: 35 50 48 131 87 288 106 534
Cuts at: 1279 1302 1828 2313 2406
Size: 23 526 485 93
Fragments arranged by size:
534 526 485 288 131 106 93 87
50 48 35 23
Bpu10I CC'TnA_GC
Cuts at: 0 1714 2406
Size: 1714 692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 2406
Size: 109 2297
BsaI GGTCTCn'nnnn_
Cuts at: 0 1557 1773 2406
Size: 1557 216 633
Fragments arranged by size:
1557 633 216
BsaAI yAC'GTr
Cuts at: 0 1447 1641 2406
Size: 1447 194 765
Fragments arranged by size:
1447 765 194
BsaHI Gr'CG_yC
Cuts at: 0 1695 1907 1969 2309 2406
Size: 1695 212 62 340 97
Fragments arranged by size:
1695 340 212 97 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 793 823 937 967 2077 2107 2406
Size: 793 30 114 30 1110 30 299
Fragments arranged by size:
1110 793 299 114 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1072 1305 2101 2269 2316 2406
Size: 1072 233 796 168 47 90
Fragments arranged by size:
1072 796 233 168 90 47
BseYI C'CCAG_C
Cuts at: 0 106 428 455 830 2406
Size: 106 322 27 375 1576
Fragments arranged by size:
1576 375 322 106 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1237 1469 1602 2406
Size: 1237 232 133 804
Fragments arranged by size:
1237 804 232 133
BsiEI CG_ry'CG
Cuts at: 0 1191 1584 1947 2406
Size: 1191 393 363 459
Fragments arranged by size:
1191 459 393 363
BsiHKAI G_wGCw'C
Cuts at: 0 1148 2406
Size: 1148 1258
BsmI GAATG_Cn'
Cuts at: 0 596 2406
Size: 596 1810
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1315 2253 2406
Size: 1315 938 153
Fragments arranged by size:
1315 938 153
Bsp1286I G_dGCh'C
Cuts at: 0 95 647 764 1129 1148 1634 1692 2062
Size: 95 552 117 365 19 486 58 370
Cuts at: 2062 2406
Size: 344
Fragments arranged by size:
552 486 370 365 344 117 95 58
19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 231 495 676 719 965 1003 1613 1679
Size: 231 264 181 43 246 38 610 66
Cuts at: 1679 2329 2406
Size: 650 77
Fragments arranged by size:
650 610 264 246 231 181 77 66
43 38
BsrBI CCG'CTC
Cuts at: 0 729 1749 2406
Size: 729 1020 657
Fragments arranged by size:
1020 729 657
BsrDI GCAATG_nn'
Cuts at: 0 1854 2406
Size: 1854 552
BsrFI r'CCGG_y
Cuts at: 0 740 1190 1339 2406
Size: 740 450 149 1067
Fragments arranged by size:
1067 740 450 149
BsrGI T'GTAC_A
Cuts at: 0 818 1994 2406
Size: 818 1176 412
Fragments arranged by size:
1176 818 412
BssSI C'ACGA_G
Cuts at: 0 308 625 1345 2406
Size: 308 317 720 1061
Fragments arranged by size:
1061 720 317 308
BstAPI GCAn_nnn'nTGC
Cuts at: 0 156 487 2321 2406
Size: 156 331 1834 85
Fragments arranged by size:
1834 331 156 85
BstXI CCAn_nnnn'nTGG
Cuts at: 0 783 1497 2406
Size: 783 714 909
Fragments arranged by size:
909 783 714
BstYI r'GATC_y
Cuts at: 0 1356 2027 2042 2368 2406
Size: 1356 671 15 326 38
Fragments arranged by size:
1356 671 326 38 15
BstZ17I GTA'TAC
Cuts at: 0 1100 2406
Size: 1100 1306
Bsu36I CC'TnA_GG
Cuts at: 0 278 2406
Size: 278 2128
BtgI C'CryG_G
Cuts at: 0 654 782 1185 1902 2406
Size: 654 128 403 717 504
Fragments arranged by size:
717 654 504 403 128
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 839 2406
Size: 839 1567
BtsI GCAGTG_nn'
Cuts at: 0 1743 2136 2406
Size: 1743 393 270
Fragments arranged by size:
1743 393 270
EaeI y'GGCC_r
Cuts at: 0 189 228 288 767 1157 1188 1434 1499
Size: 189 39 60 479 390 31 246 65
Cuts at: 1499 1541 1581 1944 2243 2406
Size: 42 40 363 299 163
Fragments arranged by size:
479 390 363 299 246 189 163 65
60 42 40 39 31
EagI C'GGCC_G
Cuts at: 0 1188 1581 1944 2406
Size: 1188 393 363 462
Fragments arranged by size:
1188 462 393 363
EarI CTCTTCn'nnn_
Cuts at: 0 1275 2406
Size: 1275 1131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2260 2406
Size: 2260 146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 85 133 264 351 639 745 871
Size: 35 50 48 131 87 288 106 126
Cuts at: 871 1279 1302 1828 2313 2328 2406
Size: 408 23 526 485 15 78
Fragments arranged by size:
526 485 408 288 131 126 106 87
78 50 48 35 23 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 277 411 1420 2193 2406
Size: 277 134 1009 773 213
Fragments arranged by size:
1009 773 277 213 134
EcoO109I rG'GnC_Cy
Cuts at: 0 27 384 402 948 1062 1536 1630 1631
Size: 27 357 18 546 114 474 94 1
Cuts at: 1631 1705 1833 2235 2406
Size: 74 128 402 171
Fragments arranged by size:
546 474 402 357 171 128 114 94
74 27 18 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 103 2406
Size: 103 2303
HaeII r_GCGC'y
Cuts at: 0 1087 1183 1698 1972 2188 2227 2406
Size: 1087 96 515 274 216 39 179
Fragments arranged by size:
1087 515 274 216 179 96 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 1341 1373 1510 1537 1542 1569
Size: 376 32 933 32 137 27 5 27
Cuts at: 1569 2002 2034 2406
Size: 433 32 372
Fragments arranged by size:
933 433 376 372 137 32 32 32
27 27 5
KasI G'GCGC_C
Cuts at: 0 1694 1968 2406
Size: 1694 274 438
Fragments arranged by size:
1694 438 274
MluI A'CGCG_T
Cuts at: 0 1118 2406
Size: 1118 1288
MscI TGG'CCA
Cuts at: 0 191 1436 2406
Size: 191 1245 970
Fragments arranged by size:
1245 970 191
MslI CAynn'nnrTG
Cuts at: 0 781 2006 2080 2406
Size: 781 1225 74 326
Fragments arranged by size:
1225 781 326 74
MspA1I CmG'CkG
Cuts at: 0 512 520 548 656 1322 1904 2214 2406
Size: 512 8 28 108 666 582 310 192
Fragments arranged by size:
666 582 512 310 192 108 28 8
NaeI GCC'GGC
Cuts at: 0 1192 1341 2406
Size: 1192 149 1065
Fragments arranged by size:
1192 1065 149
NarI GG'CG_CC
Cuts at: 0 1695 1969 2406
Size: 1695 274 437
Fragments arranged by size:
1695 437 274
NgoMIV G'CCGG_C
Cuts at: 0 1190 1339 2406
Size: 1190 149 1067
Fragments arranged by size:
1190 1067 149
NspI r_CATG'y
Cuts at: 0 481 1800 2406
Size: 481 1319 606
Fragments arranged by size:
1319 606 481
PflMI CCAn_nnn'nTGG
Cuts at: 0 462 548 2406
Size: 462 86 1858
Fragments arranged by size:
1858 462 86
PfoI T'CCnGG_A
Cuts at: 0 1813 2406
Size: 1813 593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2077 2109 2406
Size: 2077 32 297
Fragments arranged by size:
2077 297 32
PpuMI rG'GwC_Cy
Cuts at: 0 27 384 402 948 1062 1536 1833 2235
Size: 27 357 18 546 114 474 297 402
Cuts at: 2235 2406
Size: 171
Fragments arranged by size:
546 474 402 357 297 171 114 27
18
PspOMI G'GGCC_C
Cuts at: 0 1630 1688 2406
Size: 1630 58 718
Fragments arranged by size:
1630 718 58
PstI C_TGCA'G
Cuts at: 0 332 525 755 924 2406
Size: 332 193 230 169 1482
Fragments arranged by size:
1482 332 230 193 169
PvuII CAG'CTG
Cuts at: 0 520 2214 2406
Size: 520 1694 192
Fragments arranged by size:
1694 520 192
RsrII CG'GwC_CG
Cuts at: 0 651 2406
Size: 651 1755
SacII CC_GC'GG
Cuts at: 0 657 1905 2406
Size: 657 1248 501
Fragments arranged by size:
1248 657 501
SanDI GG'GwC_CC
Cuts at: 0 402 2406
Size: 402 2004
SapI GCTCTTCn'nnn_
Cuts at: 0 1275 2406
Size: 1275 1131
SbfI CC_TGCA'GG
Cuts at: 0 755 2406
Size: 755 1651
SexAI A'CCwGG_T
Cuts at: 0 1547 1781 2406
Size: 1547 234 625
Fragments arranged by size:
1547 625 234
SfcI C'TryA_G
Cuts at: 0 328 521 751 920 2406
Size: 328 193 230 169 1486
Fragments arranged by size:
1486 328 230 193 169
SfoI GGC'GCC
Cuts at: 0 1696 1970 2406
Size: 1696 274 436
Fragments arranged by size:
1696 436 274
SmaI CCC'GGG
Cuts at: 0 1390 2406
Size: 1390 1016
SmlI C'TyrA_G
Cuts at: 0 88 553 2406
Size: 88 465 1853
Fragments arranged by size:
1853 465 88
SphI G_CATG'C
Cuts at: 0 481 1800 2406
Size: 481 1319 606
Fragments arranged by size:
1319 606 481
StuI AGG'CCT
Cuts at: 0 70 568 1456 1978 2199 2406
Size: 70 498 888 522 221 207
Fragments arranged by size:
888 522 498 221 207 70
StyI C'CwwG_G
Cuts at: 0 144 1136 1416 1661 2406
Size: 144 992 280 245 745
Fragments arranged by size:
992 745 280 245 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1636 2141 2406
Size: 1636 505 265
Fragments arranged by size:
1636 505 265
TatI w'GTAC_w
Cuts at: 0 818 989 1994 2406
Size: 818 171 1005 412
Fragments arranged by size:
1005 818 412 171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 793 825 2406
Size: 793 32 1581
Fragments arranged by size:
1581 793 32
Tth111I GACn'n_nGTC
Cuts at: 0 1549 2233 2406
Size: 1549 684 173
Fragments arranged by size:
1549 684 173
XbaI T'CTAG_A
Cuts at: 0 1353 2406
Size: 1353 1053
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 783 2406
Size: 783 1623
XmaI C'CCGG_G
Cuts at: 0 1388 2406
Size: 1388 1018
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AhdI AleI AlfI
AloI AlwNI ApaI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I KasI MluI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuII RsrII SacII SanDI SapI SbfI
SexAI SfcI SfoI SmaI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI ApaLI ApoI AscI
AseI AsiSI BaeI BclI BfrBI BmtI BsaBI BsaWI
BsiWI BspEI BspHI BssHII BstBI BstEII ClaI CspCI
DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MmeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI SacI
SalI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI XhoI XmnI ZraI