Restriction map for
pFN21AE1332
(Linear) (Six-base) MAPSORT of: KIEE1332.seq Check: 1737 from: 1 to: 669
KIEE1332.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 229 398 601 669
Size: 168 61 169 203 68
Fragments arranged by size:
203 169 168 68 61
AlwNI CAG_nnn'CTG
Cuts at: 0 133 669
Size: 133 536
ApoI r'AATT_y
Cuts at: 0 261 474 607 669
Size: 261 213 133 62
Fragments arranged by size:
261 213 133 62
AvaI C'yCGr_G
Cuts at: 0 28 669
Size: 28 641
BanI G'GyrC_C
Cuts at: 0 123 556 669
Size: 123 433 113
Fragments arranged by size:
433 123 113
BanII G_rGCy'C
Cuts at: 0 121 669
Size: 121 548
BbeI G_GCGC'C
Cuts at: 0 560 669
Size: 560 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 386 669
Size: 386 283
BglI GCCn_nnn'nGGC
Cuts at: 0 418 669
Size: 418 251
BmgBI CAC'GTC
Cuts at: 0 90 669
Size: 90 579
BmrI ACTGGGnnnn_n'
Cuts at: 0 325 669
Size: 325 344
BsaBI GATnn'nnATC
Cuts at: 0 19 669
Size: 19 650
BsaHI Gr'CG_yC
Cuts at: 0 557 669
Size: 557 112
BsaWI w'CCGG_w
Cuts at: 0 543 669
Size: 543 126
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 669
Size: 121 548
BseYI C'CCAG_C
Cuts at: 0 57 93 170 669
Size: 57 36 77 499
Fragments arranged by size:
499 77 57 36
BsmI GAATG_Cn'
Cuts at: 0 539 669
Size: 539 130
Bsp1286I G_dGCh'C
Cuts at: 0 121 669
Size: 121 548
BspHI T'CATG_A
Cuts at: 0 242 669
Size: 242 427
BsrFI r'CCGG_y
Cuts at: 0 67 669
Size: 67 602
BssHII G'CGCG_C
Cuts at: 0 140 669
Size: 140 529
BtgI C'CryG_G
Cuts at: 0 434 552 647 669
Size: 434 118 95 22
Fragments arranged by size:
434 118 95 22
BtsI GCAGTG_nn'
Cuts at: 0 299 669
Size: 299 370
DraIII CAC_nnn'GTG
Cuts at: 0 294 669
Size: 294 375
EaeI y'GGCC_r
Cuts at: 0 69 296 507 669
Size: 69 227 211 162
Fragments arranged by size:
227 211 162 69
EarI CTCTTCn'nnn_
Cuts at: 0 98 197 669
Size: 98 99 472
Fragments arranged by size:
472 99 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 229 398 601 669
Size: 168 61 169 203 68
Fragments arranged by size:
203 169 168 68 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 580 669
Size: 580 89
EcoRI G'AATT_C
Cuts at: 0 261 607 669
Size: 261 346 62
Fragments arranged by size:
346 261 62
HaeII r_GCGC'y
Cuts at: 0 81 133 560 669
Size: 81 52 427 109
Fragments arranged by size:
427 109 81 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 355 387 669
Size: 316 32 7 32 282
Fragments arranged by size:
316 282 32 32 7
KasI G'GCGC_C
Cuts at: 0 556 669
Size: 556 113
MscI TGG'CCA
Cuts at: 0 298 509 669
Size: 298 211 160
Fragments arranged by size:
298 211 160
MslI CAynn'nnrTG
Cuts at: 0 515 669
Size: 515 154
NaeI GCC'GGC
Cuts at: 0 69 669
Size: 69 600
NarI GG'CG_CC
Cuts at: 0 557 669
Size: 557 112
NcoI C'CATG_G
Cuts at: 0 552 669
Size: 552 117
NgoMIV G'CCGG_C
Cuts at: 0 67 669
Size: 67 602
PstI C_TGCA'G
Cuts at: 0 604 669
Size: 604 65
SapI GCTCTTCn'nnn_
Cuts at: 0 197 669
Size: 197 472
SfcI C'TryA_G
Cuts at: 0 600 669
Size: 600 69
SfoI GGC'GCC
Cuts at: 0 558 669
Size: 558 111
SmlI C'TyrA_G
Cuts at: 0 7 669
Size: 7 662
StyI C'CwwG_G
Cuts at: 0 552 593 669
Size: 552 41 76
Fragments arranged by size:
552 76 41
TatI w'GTAC_w
Cuts at: 0 236 669
Size: 236 433
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 252 284 669
Size: 252 32 385
Fragments arranged by size:
385 252 32
Enzymes that do cut:
AcuI AlwNI ApoI AvaI BanI BanII BbeI BbsI
BglI BmgBI BmrI BsaBI BsaHI BsaWI BseRI BseYI
BsmI Bsp1286I BspHI BsrFI BssHII BtgI BtsI DraIII
EaeI EarI Eco57MI EcoNI EcoRI HaeII Hin4I KasI
MscI MslI NaeI NarI NcoI NgoMIV PstI SapI
SfcI SfoI SmlI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoO109I
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MspA1I NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI