Restriction map for
pFN21AE1329
(Linear) (Six-base) MAPSORT of: KIEE1329.seq Check: 7019 from: 1 to: 1791
KIEE1329.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AccI GT'mk_AC
Cuts at: 0 626 1791
Size: 626 1165
Acc65I G'GTAC_C
Cuts at: 0 335 1791
Size: 335 1456
AfeI AGC'GCT
Cuts at: 0 1489 1791
Size: 1489 302
AflIII A'CryG_T
Cuts at: 0 347 1791
Size: 347 1444
AleI CACnn'nnGTG
Cuts at: 0 655 1791
Size: 655 1136
AlwNI CAG_nnn'CTG
Cuts at: 0 792 1248 1791
Size: 792 456 543
Fragments arranged by size:
792 543 456
ApaI G_GGCC'C
Cuts at: 0 1133 1349 1791
Size: 1133 216 442
Fragments arranged by size:
1133 442 216
ApaLI G'TGCA_C
Cuts at: 0 1357 1791
Size: 1357 434
AvaI C'yCGr_G
Cuts at: 0 895 1058 1102 1491 1791
Size: 895 163 44 389 300
Fragments arranged by size:
895 389 300 163 44
BamHI G'GATC_C
Cuts at: 0 164 1791
Size: 164 1627
BanI G'GyrC_C
Cuts at: 0 277 335 616 1387 1791
Size: 277 58 281 771 404
Fragments arranged by size:
771 404 281 277 58
BanII G_rGCy'C
Cuts at: 0 395 1133 1349 1791
Size: 395 738 216 442
Fragments arranged by size:
738 442 395 216
BbeI G_GCGC'C
Cuts at: 0 620 1791
Size: 620 1171
BbsI GAAGACnn'nnnn_
Cuts at: 0 598 1468 1791
Size: 598 870 323
Fragments arranged by size:
870 598 323
BbvCI CC'TCA_GC
Cuts at: 0 421 1294 1791
Size: 421 873 497
Fragments arranged by size:
873 497 421
BciVI GTATCCnnnnn_n'
Cuts at: 0 711 1791
Size: 711 1080
BglI GCCn_nnn'nGGC
Cuts at: 0 1385 1791
Size: 1385 406
BglII A'GATC_T
Cuts at: 0 1229 1791
Size: 1229 562
Bme1580I G_kGCm'C
Cuts at: 0 1133 1349 1361 1791
Size: 1133 216 12 430
Fragments arranged by size:
1133 430 216 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 92 1268 1539 1791
Size: 92 1176 271 252
Fragments arranged by size:
1176 271 252 92
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 627 1440 1791
Size: 627 813 351
Fragments arranged by size:
813 627 351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 1009 1594 1791
Size: 452 557 585 197
Fragments arranged by size:
585 557 452 197
Bpu10I CC'TnA_GC
Cuts at: 0 421 490 1249 1294 1791
Size: 421 69 759 45 497
Fragments arranged by size:
759 497 421 69 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 277 1765 1791
Size: 43 234 1488 26
Fragments arranged by size:
1488 234 43 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 20 43 544 1200 1208 1791
Size: 20 23 501 656 8 583
Fragments arranged by size:
656 583 501 23 20 8
BsaAI yAC'GTr
Cuts at: 0 1533 1791
Size: 1533 258
BsaHI Gr'CG_yC
Cuts at: 0 617 1791
Size: 617 1174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 425 455 1501 1531 1594 1624 1678 1708
Size: 425 30 1046 30 63 30 54 30
Cuts at: 1708 1791
Size: 83
Fragments arranged by size:
1046 425 83 63 54 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 619 757 1741 1791
Size: 619 138 984 50
Fragments arranged by size:
984 619 138 50
BseYI C'CCAG_C
Cuts at: 0 1632 1791
Size: 1632 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1522 1690 1791
Size: 1522 168 101
Fragments arranged by size:
1522 168 101
BsiEI CG_ry'CG
Cuts at: 0 1380 1791
Size: 1380 411
BsiHKAI G_wGCw'C
Cuts at: 0 455 1361 1791
Size: 455 906 430
Fragments arranged by size:
906 455 430
BsmI GAATG_Cn'
Cuts at: 0 666 1650 1791
Size: 666 984 141
Fragments arranged by size:
984 666 141
BsmBI CGTCTCn'nnnn_
Cuts at: 0 361 1791
Size: 361 1430
Bsp1286I G_dGCh'C
Cuts at: 0 395 455 1133 1349 1361 1791
Size: 395 60 678 216 12 430
Fragments arranged by size:
678 430 395 216 60 12
BspHI T'CATG_A
Cuts at: 0 1049 1791
Size: 1049 742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 638 1712 1791
Size: 638 1074 79
Fragments arranged by size:
1074 638 79
BsrBI CCG'CTC
Cuts at: 0 489 1791
Size: 489 1302
BsrFI r'CCGG_y
Cuts at: 0 1369 1791
Size: 1369 422
BssSI C'ACGA_G
Cuts at: 0 651 1791
Size: 651 1140
BstEII G'GTnAC_C
Cuts at: 0 150 495 1791
Size: 150 345 1296
Fragments arranged by size:
1296 345 150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 352 1437 1473 1791
Size: 352 1085 36 318
Fragments arranged by size:
1085 352 318 36
BstYI r'GATC_y
Cuts at: 0 164 1229 1791
Size: 164 1065 562
Fragments arranged by size:
1065 562 164
Bsu36I CC'TnA_GG
Cuts at: 0 612 1791
Size: 612 1179
BtgI C'CryG_G
Cuts at: 0 9 75 339 995 1263 1791
Size: 9 66 264 656 268 528
Fragments arranged by size:
656 528 268 264 66 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 954 1524 1791
Size: 954 570 267
Fragments arranged by size:
954 570 267
BtsI GCAGTG_nn'
Cuts at: 0 1359 1791
Size: 1359 432
DrdI GACnn_nn'nnGTC
Cuts at: 0 1267 1791
Size: 1267 524
EaeI y'GGCC_r
Cuts at: 0 342 805 1377 1535 1791
Size: 342 463 572 158 256
Fragments arranged by size:
572 463 342 256 158
EagI C'GGCC_G
Cuts at: 0 1377 1791
Size: 1377 414
EarI CTCTTCn'nnn_
Cuts at: 0 1759 1791
Size: 1759 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 1009 1594 1791
Size: 452 557 585 197
Fragments arranged by size:
585 557 452 197
EcoNI CCTnn'n_nnAGG
Cuts at: 0 58 1340 1791
Size: 58 1282 451
Fragments arranged by size:
1282 451 58
EcoO109I rG'GnC_Cy
Cuts at: 0 943 1130 1269 1345 1434 1578 1791
Size: 943 187 139 76 89 144 213
Fragments arranged by size:
943 213 187 144 139 89 76
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 750 1791
Size: 750 1041
HaeII r_GCGC'y
Cuts at: 0 620 1370 1491 1791
Size: 620 750 121 300
Fragments arranged by size:
750 620 300 121
KasI G'GCGC_C
Cuts at: 0 616 1791
Size: 616 1175
KpnI G_GTAC'C
Cuts at: 0 339 1791
Size: 339 1452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 926 1586 1791
Size: 668 258 660 205
Fragments arranged by size:
668 660 258 205
MscI TGG'CCA
Cuts at: 0 344 1537 1791
Size: 344 1193 254
Fragments arranged by size:
1193 344 254
MslI CAynn'nnrTG
Cuts at: 0 655 1048 1354 1405 1411 1471 1531 1791
Size: 655 393 306 51 6 60 60 260
Fragments arranged by size:
655 393 306 260 60 60 51 6
MspA1I CmG'CkG
Cuts at: 0 303 804 936 1203 1320 1746 1791
Size: 303 501 132 267 117 426 45
Fragments arranged by size:
501 426 303 267 132 117 45
NaeI GCC'GGC
Cuts at: 0 1371 1791
Size: 1371 420
NarI GG'CG_CC
Cuts at: 0 617 1791
Size: 617 1174
NcoI C'CATG_G
Cuts at: 0 339 1263 1791
Size: 339 924 528
Fragments arranged by size:
924 528 339
NgoMIV G'CCGG_C
Cuts at: 0 1369 1791
Size: 1369 422
NspI r_CATG'y
Cuts at: 0 351 1116 1410 1791
Size: 351 765 294 381
Fragments arranged by size:
765 381 351 294
PciI A'CATG_T
Cuts at: 0 347 1791
Size: 347 1444
PflMI CCAn_nnn'nTGG
Cuts at: 0 792 1791
Size: 792 999
PfoI T'CCnGG_A
Cuts at: 0 1301 1791
Size: 1301 490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1758 1790 1791
Size: 1758 32 1
Fragments arranged by size:
1758 32 1
PpuMI rG'GwC_Cy
Cuts at: 0 1269 1578 1791
Size: 1269 309 213
Fragments arranged by size:
1269 309 213
PspOMI G'GGCC_C
Cuts at: 0 1129 1345 1791
Size: 1129 216 446
Fragments arranged by size:
1129 446 216
PstI C_TGCA'G
Cuts at: 0 266 380 875 1366 1586 1791
Size: 266 114 495 491 220 205
Fragments arranged by size:
495 491 266 220 205 114
PvuII CAG'CTG
Cuts at: 0 936 1791
Size: 936 855
SanDI GG'GwC_CC
Cuts at: 0 1269 1791
Size: 1269 522
SapI GCTCTTCn'nnn_
Cuts at: 0 1759 1791
Size: 1759 32
SbfI CC_TGCA'GG
Cuts at: 0 266 875 1791
Size: 266 609 916
Fragments arranged by size:
916 609 266
ScaI AGT'ACT
Cuts at: 0 238 1791
Size: 238 1553
SexAI A'CCwGG_T
Cuts at: 0 572 1791
Size: 572 1219
SfcI C'TryA_G
Cuts at: 0 262 376 871 1362 1582 1791
Size: 262 114 495 491 220 209
Fragments arranged by size:
495 491 262 220 209 114
SfoI GGC'GCC
Cuts at: 0 618 1791
Size: 618 1173
SmaI CCC'GGG
Cuts at: 0 897 1791
Size: 897 894
SmlI C'TyrA_G
Cuts at: 0 58 292 1780 1791
Size: 58 234 1488 11
Fragments arranged by size:
1488 234 58 11
SpeI A'CTAG_T
Cuts at: 0 322 1791
Size: 322 1469
SphI G_CATG'C
Cuts at: 0 1116 1791
Size: 1116 675
StuI AGG'CCT
Cuts at: 0 610 1660 1791
Size: 610 1050 131
Fragments arranged by size:
1050 610 131
StyI C'CwwG_G
Cuts at: 0 339 383 467 725 1263 1430 1655 1791
Size: 339 44 84 258 538 167 225 136
Fragments arranged by size:
538 339 258 225 167 136 84 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2 514 529 1791
Size: 2 512 15 1262
Fragments arranged by size:
1262 512 15 2
TatI w'GTAC_w
Cuts at: 0 236 1517 1791
Size: 236 1281 274
Fragments arranged by size:
1281 274 236
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 425 457 1791
Size: 425 32 1334
Fragments arranged by size:
1334 425 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 469 1062 1434 1791
Size: 469 593 372 357
Fragments arranged by size:
593 469 372 357
XmaI C'CCGG_G
Cuts at: 0 895 1791
Size: 895 896
Enzymes that do cut:
AccI Acc65I AfeI AflIII AleI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI
HaeII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PpiI
PpuMI PspOMI PstI PvuII SanDI SapI SbfI ScaI
SexAI SfcI SfoI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AgeI AhdI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BcgI
BclI BfrBI BlpI BmgBI BmtI BsaBI BsaWI BsiWI
BspEI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I ClaI
CspCI DraI DraIII EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PasI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SfiI SgfI SgrAI SnaBI SrfI SspI
SwaI Tth111I XbaI XhoI XmnI ZraI