Restriction map for
pFN21AE1326
(Linear) (Six-base) MAPSORT of: KIEE1326.seq Check: 648 from: 1 to: 1722
KIEE1326.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AatII G_ACGT'C
Cuts at: 0 806 1722
Size: 806 916
Acc65I G'GTAC_C
Cuts at: 0 1457 1722
Size: 1457 265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1286 1722
Size: 1286 436
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7 39 553 585 1722
Size: 7 32 514 32 1137
Fragments arranged by size:
1137 514 32 32 7
AlwNI CAG_nnn'CTG
Cuts at: 0 150 180 549 1119 1449 1722
Size: 150 30 369 570 330 273
Fragments arranged by size:
570 369 330 273 150 30
ApaI G_GGCC'C
Cuts at: 0 771 1722
Size: 771 951
AvaI C'yCGr_G
Cuts at: 0 784 1274 1466 1722
Size: 784 490 192 256
Fragments arranged by size:
784 490 256 192
AvrII C'CTAG_G
Cuts at: 0 1017 1722
Size: 1017 705
BamHI G'GATC_C
Cuts at: 0 494 1722
Size: 494 1228
BanI G'GyrC_C
Cuts at: 0 1457 1722
Size: 1457 265
BanII G_rGCy'C
Cuts at: 0 93 245 541 668 771 1722
Size: 93 152 296 127 103 951
Fragments arranged by size:
951 296 152 127 103 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 1527 1722
Size: 1527 195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 361 395 865 899 1722
Size: 361 34 470 34 823
Fragments arranged by size:
823 470 361 34 34
BclI T'GATC_A
Cuts at: 0 320 1722
Size: 320 1402
BglI GCCn_nnn'nGGC
Cuts at: 0 130 749 892 1279 1573 1722
Size: 130 619 143 387 294 149
Fragments arranged by size:
619 387 294 149 143 130
BlpI GC'TnA_GC
Cuts at: 0 1437 1711 1722
Size: 1437 274 11
Fragments arranged by size:
1437 274 11
Bme1580I G_kGCm'C
Cuts at: 0 157 771 1722
Size: 157 614 951
Fragments arranged by size:
951 614 157
BmgBI CAC'GTC
Cuts at: 0 879 1722
Size: 879 843
BmrI ACTGGGnnnn_n'
Cuts at: 0 500 1722
Size: 500 1222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 1000 1141 1544 1722
Size: 77 923 141 403 178
Fragments arranged by size:
923 403 178 141 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1722
Size: 295 1427
BsaI GGTCTCn'nnnn_
Cuts at: 0 51 803 1640 1722
Size: 51 752 837 82
Fragments arranged by size:
837 752 82 51
BsaBI GATnn'nnATC
Cuts at: 0 1586 1722
Size: 1586 136
BsaHI Gr'CG_yC
Cuts at: 0 731 803 1722
Size: 731 72 919
Fragments arranged by size:
919 731 72
BsaWI w'CCGG_w
Cuts at: 0 477 572 906 1722
Size: 477 95 334 816
Fragments arranged by size:
816 477 334 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 220 250 1637 1667 1722
Size: 220 30 1387 30 55
Fragments arranged by size:
1387 220 55 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1330 1544 1722
Size: 1330 214 178
Fragments arranged by size:
1330 214 178
BseYI C'CCAG_C
Cuts at: 0 150 1032 1442 1722
Size: 150 882 410 280
Fragments arranged by size:
882 410 280 150
Bsp1286I G_dGCh'C
Cuts at: 0 93 157 245 541 668 771 1722
Size: 93 64 88 296 127 103 951
Fragments arranged by size:
951 296 127 103 93 88 64
BspEI T'CCGG_A
Cuts at: 0 572 906 1722
Size: 572 334 816
Fragments arranged by size:
816 572 334
BspHI T'CATG_A
Cuts at: 0 1700 1722
Size: 1700 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 415 821 1722
Size: 415 406 901
Fragments arranged by size:
901 415 406
BsrBI CCG'CTC
Cuts at: 0 1436 1493 1722
Size: 1436 57 229
Fragments arranged by size:
1436 229 57
BsrDI GCAATG_nn'
Cuts at: 0 269 338 1478 1722
Size: 269 69 1140 244
Fragments arranged by size:
1140 269 244 69
BsrFI r'CCGG_y
Cuts at: 0 443 633 1301 1722
Size: 443 190 668 421
Fragments arranged by size:
668 443 421 190
BssSI C'ACGA_G
Cuts at: 0 370 1722
Size: 370 1352
BstAPI GCAn_nnn'nTGC
Cuts at: 0 180 1480 1722
Size: 180 1300 242
Fragments arranged by size:
1300 242 180
BstEII G'GTnAC_C
Cuts at: 0 708 1359 1722
Size: 708 651 363
Fragments arranged by size:
708 651 363
BstXI CCAn_nnnn'nTGG
Cuts at: 0 462 879 1722
Size: 462 417 843
Fragments arranged by size:
843 462 417
BstYI r'GATC_y
Cuts at: 0 482 494 1028 1722
Size: 482 12 534 694
Fragments arranged by size:
694 534 482 12
BtgI C'CryG_G
Cuts at: 0 245 299 360 449 1626 1722
Size: 245 54 61 89 1177 96
Fragments arranged by size:
1177 245 96 89 61 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 375 441 1181 1722
Size: 375 66 740 541
Fragments arranged by size:
740 541 375 66
BtsI GCAGTG_nn'
Cuts at: 0 155 1298 1722
Size: 155 1143 424
Fragments arranged by size:
1143 424 155
DraIII CAC_nnn'GTG
Cuts at: 0 599 1722
Size: 599 1123
EaeI y'GGCC_r
Cuts at: 0 452 1144 1431 1722
Size: 452 692 287 291
Fragments arranged by size:
692 452 291 287
EarI CTCTTCn'nnn_
Cuts at: 0 955 1722
Size: 955 767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 1000 1141 1544 1722
Size: 77 923 141 403 178
Fragments arranged by size:
923 403 178 141 77
EcoO109I rG'GnC_Cy
Cuts at: 0 767 1014 1722
Size: 767 247 708
Fragments arranged by size:
767 708 247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1251 1722
Size: 1251 471
HaeII r_GCGC'y
Cuts at: 0 309 1077 1336 1722
Size: 309 768 259 386
Fragments arranged by size:
768 386 309 259
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 75 107 220 252 467 499
Size: 4 32 39 32 113 32 215 32
Cuts at: 499 905 937 1722
Size: 406 32 785
Fragments arranged by size:
785 406 215 113 39 32 32 32
32 32 4
KpnI G_GTAC'C
Cuts at: 0 1461 1722
Size: 1461 261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1623 1722
Size: 497 1126 99
Fragments arranged by size:
1126 497 99
MscI TGG'CCA
Cuts at: 0 454 1722
Size: 454 1268
MslI CAynn'nnrTG
Cuts at: 0 1000 1114 1414 1705 1722
Size: 1000 114 300 291 17
Fragments arranged by size:
1000 300 291 114 17
MspA1I CmG'CkG
Cuts at: 0 106 180 187 1308 1722
Size: 106 74 7 1121 414
Fragments arranged by size:
1121 414 106 74 7
NspI r_CATG'y
Cuts at: 0 1267 1722
Size: 1267 455
PflMI CCAn_nnn'nTGG
Cuts at: 0 150 549 1722
Size: 150 399 1173
Fragments arranged by size:
1173 399 150
PfoI T'CCnGG_A
Cuts at: 0 8 93 206 401 977 1722
Size: 8 85 113 195 576 745
Fragments arranged by size:
745 576 195 113 85 8
PpuMI rG'GwC_Cy
Cuts at: 0 1014 1722
Size: 1014 708
PspOMI G'GGCC_C
Cuts at: 0 767 1722
Size: 767 955
PstI C_TGCA'G
Cuts at: 0 32 503 1722
Size: 32 471 1219
Fragments arranged by size:
1219 471 32
PvuII CAG'CTG
Cuts at: 0 180 187 1308 1722
Size: 180 7 1121 414
Fragments arranged by size:
1121 414 180 7
SbfI CC_TGCA'GG
Cuts at: 0 503 1722
Size: 503 1219
SexAI A'CCwGG_T
Cuts at: 0 1649 1722
Size: 1649 73
SfcI C'TryA_G
Cuts at: 0 28 499 1722
Size: 28 471 1223
Fragments arranged by size:
1223 471 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 130 1722
Size: 130 1592
SmaI CCC'GGG
Cuts at: 0 786 1276 1722
Size: 786 490 446
Fragments arranged by size:
786 490 446
SmlI C'TyrA_G
Cuts at: 0 310 1722
Size: 310 1412
SphI G_CATG'C
Cuts at: 0 1267 1722
Size: 1267 455
SrfI GCCC'GGGC
Cuts at: 0 786 1722
Size: 786 936
SspI AAT'ATT
Cuts at: 0 618 1722
Size: 618 1104
StyI C'CwwG_G
Cuts at: 0 470 1017 1226 1722
Size: 470 547 209 496
Fragments arranged by size:
547 496 470 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 26 591 1722
Size: 26 565 1131
Fragments arranged by size:
1131 565 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1637 1669 1722
Size: 1637 32 53
Fragments arranged by size:
1637 53 32
Tth111I GACn'n_nGTC
Cuts at: 0 844 1722
Size: 844 878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1116 1182 1374 1568 1722
Size: 1116 66 192 194 154
Fragments arranged by size:
1116 194 192 154 66
XmaI C'CCGG_G
Cuts at: 0 784 1274 1722
Size: 784 490 448
Fragments arranged by size:
784 490 448
XmnI GAAnn'nnTTC
Cuts at: 0 1045 1722
Size: 1045 677
ZraI GAC'GTC
Cuts at: 0 804 1722
Size: 804 918
Enzymes that do cut:
AatII Acc65I AlfI AloI AlwNI ApaI AvaI AvrII
BamHI BanI BanII BbsI BcgI BclI BglI BlpI
Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI DraIII EaeI EarI Eco57MI EcoO109I
FalI HaeII Hin4I KpnI MmeI MscI MslI MspA1I
NspI PflMI PfoI PpuMI PspOMI PstI PvuII SbfI
SexAI SfcI SfiI SmaI SmlI SphI SrfI SspI
StyI TaqII TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI ApaLI ApoI AscI AseI AsiSI BaeI
BbeI BbvCI BciVI BfrBI BglII BmtI BplI Bpu10I
BsaAI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BsrGI
BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI ScaI SfoI SgfI SgrAI SnaBI SpeI StuI
SwaI TatI XbaI XhoI