Restriction map for
pFN21AE1325
(Linear) (Six-base) MAPSORT of: KIEE1325.seq Check: 301 from: 1 to: 1506
KIEE1325.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AccI GT'mk_AC
Cuts at: 0 317 1506
Size: 317 1189
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1506
Size: 253 1253
AfeI AGC'GCT
Cuts at: 0 1298 1364 1506
Size: 1298 66 142
Fragments arranged by size:
1298 142 66
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 52 84 1506
Size: 52 32 1422
Fragments arranged by size:
1422 52 32
AlwNI CAG_nnn'CTG
Cuts at: 0 300 652 1123 1506
Size: 300 352 471 383
Fragments arranged by size:
471 383 352 300
ApaI G_GGCC'C
Cuts at: 0 13 417 1099 1350 1407 1506
Size: 13 404 682 251 57 99
Fragments arranged by size:
682 404 251 99 57 13
ApaLI G'TGCA_C
Cuts at: 0 716 1506
Size: 716 790
ApoI r'AATT_y
Cuts at: 0 1322 1506
Size: 1322 184
AvaI C'yCGr_G
Cuts at: 0 28 959 1067 1506
Size: 28 931 108 439
Fragments arranged by size:
931 439 108 28
BanI G'GyrC_C
Cuts at: 0 923 1257 1506
Size: 923 334 249
Fragments arranged by size:
923 334 249
BanII G_rGCy'C
Cuts at: 0 13 417 521 644 1099 1350 1360 1387
Size: 13 404 104 123 455 251 10 27
Cuts at: 1387 1407 1506
Size: 20 99
Fragments arranged by size:
455 404 251 123 104 99 27 20
13 10
BbeI G_GCGC'C
Cuts at: 0 927 1506
Size: 927 579
BglI GCCn_nnn'nGGC
Cuts at: 0 263 412 1094 1506
Size: 263 149 682 412
Fragments arranged by size:
682 412 263 149
BglII A'GATC_T
Cuts at: 0 795 1506
Size: 795 711
BlpI GC'TnA_GC
Cuts at: 0 182 1359 1506
Size: 182 1177 147
Fragments arranged by size:
1177 182 147
Bme1580I G_kGCm'C
Cuts at: 0 13 417 720 1099 1350 1407 1506
Size: 13 404 303 379 251 57 99
Fragments arranged by size:
404 379 303 251 99 57 13
BmgBI CAC'GTC
Cuts at: 0 777 1506
Size: 777 729
BmrI ACTGGGnnnn_n'
Cuts at: 0 716 1506
Size: 716 790
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 321 535 1506
Size: 321 214 971
Fragments arranged by size:
971 321 214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 197 374 548 798 919 1100 1178
Size: 64 133 177 174 250 121 181 78
Cuts at: 1178 1506
Size: 328
Fragments arranged by size:
328 250 181 177 174 133 121 78
64
Bpu10I CC'TnA_GC
Cuts at: 0 681 1506
Size: 681 825
BsaI GGTCTCn'nnnn_
Cuts at: 0 542 847 1506
Size: 542 305 659
Fragments arranged by size:
659 542 305
BsaAI yAC'GTr
Cuts at: 0 747 1416 1506
Size: 747 669 90
Fragments arranged by size:
747 669 90
BsaHI Gr'CG_yC
Cuts at: 0 468 848 924 1506
Size: 468 380 76 582
Fragments arranged by size:
582 468 380 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 52 82 1506
Size: 52 30 1424
Fragments arranged by size:
1424 52 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 53 67 527 977 1437 1506
Size: 53 14 460 450 460 69
Fragments arranged by size:
460 460 450 69 53 14
BseYI C'CCAG_C
Cuts at: 0 645 663 836 1210 1506
Size: 645 18 173 374 296
Fragments arranged by size:
645 374 296 173 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 1506
Size: 442 1064
BsiHKAI G_wGCw'C
Cuts at: 0 45 188 720 1360 1387 1506
Size: 45 143 532 640 27 119
Fragments arranged by size:
640 532 143 119 45 27
Bsp1286I G_dGCh'C
Cuts at: 0 13 45 188 417 521 644 720 1099
Size: 13 32 143 229 104 123 76 379
Cuts at: 1099 1350 1360 1387 1407 1506
Size: 251 10 27 20 99
Fragments arranged by size:
379 251 229 143 123 104 99 76
32 27 20 13 10
BsrBI CCG'CTC
Cuts at: 0 576 1506
Size: 576 930
BssSI C'ACGA_G
Cuts at: 0 88 418 621 1506
Size: 88 330 203 885
Fragments arranged by size:
885 330 203 88
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1419 1506
Size: 1419 87
BstYI r'GATC_y
Cuts at: 0 795 1506
Size: 795 711
BtgI C'CryG_G
Cuts at: 0 636 1506
Size: 636 870
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1490 1506
Size: 1490 16
BtsI GCAGTG_nn'
Cuts at: 0 140 1021 1138 1506
Size: 140 881 117 368
Fragments arranged by size:
881 368 140 117
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 129 164 1506
Size: 129 35 1342
Fragments arranged by size:
1342 129 35
DraIII CAC_nnn'GTG
Cuts at: 0 724 1506
Size: 724 782
EaeI y'GGCC_r
Cuts at: 0 137 1506
Size: 137 1369
EarI CTCTTCn'nnn_
Cuts at: 0 497 504 1420 1506
Size: 497 7 916 86
Fragments arranged by size:
916 497 86 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 929 1506
Size: 244 685 577
Fragments arranged by size:
685 577 244
EcoICRI GAG'CTC
Cuts at: 0 1358 1385 1506
Size: 1358 27 121
Fragments arranged by size:
1358 121 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 197 253 374 548 798 919 1100
Size: 64 133 56 121 174 250 121 181
Cuts at: 1100 1178 1506
Size: 78 328
Fragments arranged by size:
328 250 181 174 133 121 121 78
64 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 421 1506
Size: 79 342 1085
Fragments arranged by size:
1085 342 79
EcoO109I rG'GnC_Cy
Cuts at: 0 9 414 559 756 1072 1095 1096 1269
Size: 9 405 145 197 316 23 1 173
Cuts at: 1269 1346 1347 1403 1404 1506
Size: 77 1 56 1 102
Fragments arranged by size:
405 316 197 173 145 102 77 56
23 9 1 1 1
EcoRI G'AATT_C
Cuts at: 0 1322 1506
Size: 1322 184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 264 1506
Size: 264 1242
HaeII r_GCGC'y
Cuts at: 0 169 927 1300 1366 1506
Size: 169 758 373 66 140
Fragments arranged by size:
758 373 169 140 66
KasI G'GCGC_C
Cuts at: 0 923 1506
Size: 923 583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1475 1506
Size: 1475 31
MscI TGG'CCA
Cuts at: 0 139 1506
Size: 139 1367
MslI CAynn'nnrTG
Cuts at: 0 542 1506
Size: 542 964
MspA1I CmG'CkG
Cuts at: 0 49 156 667 1329 1506
Size: 49 107 511 662 177
Fragments arranged by size:
662 511 177 107 49
NarI GG'CG_CC
Cuts at: 0 924 1506
Size: 924 582
NspI r_CATG'y
Cuts at: 0 739 1506
Size: 739 767
PasI CC'CwG_GG
Cuts at: 0 582 1342 1506
Size: 582 760 164
Fragments arranged by size:
760 582 164
PflMI CCAn_nnn'nTGG
Cuts at: 0 652 1123 1156 1506
Size: 652 471 33 350
Fragments arranged by size:
652 471 350 33
PfoI T'CCnGG_A
Cuts at: 0 80 1216 1506
Size: 80 1136 290
Fragments arranged by size:
1136 290 80
PmlI CAC'GTG
Cuts at: 0 747 1416 1506
Size: 747 669 90
Fragments arranged by size:
747 669 90
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 1506
Size: 52 32 1422
Fragments arranged by size:
1422 52 32
PpuMI rG'GwC_Cy
Cuts at: 0 756 1072 1506
Size: 756 316 434
Fragments arranged by size:
756 434 316
PspOMI G'GGCC_C
Cuts at: 0 9 413 1095 1346 1403 1506
Size: 9 404 682 251 57 103
Fragments arranged by size:
682 404 251 103 57 9
PstI C_TGCA'G
Cuts at: 0 147 1370 1506
Size: 147 1223 136
Fragments arranged by size:
1223 147 136
PvuII CAG'CTG
Cuts at: 0 156 667 1329 1506
Size: 156 511 662 177
Fragments arranged by size:
662 511 177 156
SacI G_AGCT'C
Cuts at: 0 1360 1387 1506
Size: 1360 27 119
Fragments arranged by size:
1360 119 27
SanDI GG'GwC_CC
Cuts at: 0 756 1072 1506
Size: 756 316 434
Fragments arranged by size:
756 434 316
SfcI C'TryA_G
Cuts at: 0 143 1366 1506
Size: 143 1223 140
Fragments arranged by size:
1223 143 140
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 412 1094 1506
Size: 412 682 412
Fragments arranged by size:
682 412 412
SfoI GGC'GCC
Cuts at: 0 925 1506
Size: 925 581
SmaI CCC'GGG
Cuts at: 0 30 1069 1506
Size: 30 1039 437
Fragments arranged by size:
1039 437 30
SmlI C'TyrA_G
Cuts at: 0 959 1506
Size: 959 547
StuI AGG'CCT
Cuts at: 0 406 1506
Size: 406 1100
StyI C'CwwG_G
Cuts at: 0 194 552 1506
Size: 194 358 954
Fragments arranged by size:
954 358 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 735 1188 1506
Size: 735 453 318
Fragments arranged by size:
735 453 318
TatI w'GTAC_w
Cuts at: 0 302 1506
Size: 302 1204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 761 793 1506
Size: 761 32 713
Fragments arranged by size:
761 713 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 349 1506
Size: 349 1157
XhoI C'TCGA_G
Cuts at: 0 959 1506
Size: 959 547
XmaI C'CCGG_G
Cuts at: 0 28 1067 1506
Size: 28 1039 439
Fragments arranged by size:
1039 439 28
Enzymes that do cut:
AccI AcuI AfeI AloI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI BssSI
BstAPI BstYI BtgI BtgZI BtsI CspCI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII KasI MmeI MscI MslI MspA1I NarI NspI
PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI
PvuII SacI SanDI SfcI SfiI SfoI SmaI SmlI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BfrBI BmtI BpuEI
BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI
BstZ17I Bsu36I ClaI DraI DrdI EagI EcoRV FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XmnI ZraI