Restriction map for
pFN21AE1324
(Linear) (Six-base) MAPSORT of: KIEE1324.seq Check: 3179 from: 1 to: 2442
KIEE1324.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 412 527 710 2203 2442
Size: 412 115 183 1493 239
Fragments arranged by size:
1493 412 239 183 115
Acc65I G'GTAC_C
Cuts at: 0 296 2147 2391 2442
Size: 296 1851 244 51
Fragments arranged by size:
1851 296 244 51
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 166 463 1152 2442
Size: 130 36 297 689 1290
Fragments arranged by size:
1290 689 297 130 36
AhdI GACnn_n'nnGTC
Cuts at: 0 1993 2442
Size: 1993 449
AleI CACnn'nnGTG
Cuts at: 0 316 533 2442
Size: 316 217 1909
Fragments arranged by size:
1909 316 217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 67 1858 2442
Size: 67 1791 584
Fragments arranged by size:
1791 584 67
AlwNI CAG_nnn'CTG
Cuts at: 0 702 1175 1770 2015 2144 2371 2442
Size: 702 473 595 245 129 227 71
Fragments arranged by size:
702 595 473 245 227 129 71
ApaI G_GGCC'C
Cuts at: 0 24 1191 2256 2442
Size: 24 1167 1065 186
Fragments arranged by size:
1167 1065 186 24
ApaLI G'TGCA_C
Cuts at: 0 1373 1843 2442
Size: 1373 470 599
Fragments arranged by size:
1373 599 470
ApoI r'AATT_y
Cuts at: 0 1471 2442
Size: 1471 971
AvaI C'yCGr_G
Cuts at: 0 117 314 1655 2442
Size: 117 197 1341 787
Fragments arranged by size:
1341 787 197 117
AvrII C'CTAG_G
Cuts at: 0 1515 2442
Size: 1515 927
BanI G'GyrC_C
Cuts at: 0 296 2037 2134 2147 2339 2391 2442
Size: 296 1741 97 13 192 52 51
Fragments arranged by size:
1741 296 192 97 52 51 13
BanII G_rGCy'C
Cuts at: 0 24 32 128 137 203 416 1191 1532
Size: 24 8 96 9 66 213 775 341
Cuts at: 1532 1617 1780 2256 2442
Size: 85 163 476 186
Fragments arranged by size:
775 476 341 213 186 163 96 85
66 24 9 8
BbvCI CC'TCA_GC
Cuts at: 0 1363 2098 2442
Size: 1363 735 344
Fragments arranged by size:
1363 735 344
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1961 1995 2442
Size: 1961 34 447
Fragments arranged by size:
1961 447 34
BclI T'GATC_A
Cuts at: 0 663 2442
Size: 663 1779
BglI GCCn_nnn'nGGC
Cuts at: 0 60 1427 2260 2442
Size: 60 1367 833 182
Fragments arranged by size:
1367 833 182 60
BglII A'GATC_T
Cuts at: 0 510 2442
Size: 510 1932
Bme1580I G_kGCm'C
Cuts at: 0 24 540 870 903 1191 1377 1847 1962
Size: 24 516 330 33 288 186 470 115
Cuts at: 1962 2040 2157 2256 2344 2442
Size: 78 117 99 88 98
Fragments arranged by size:
516 470 330 288 186 117 115 99
98 88 78 33 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 1488 1685 2442
Size: 1488 197 757
Fragments arranged by size:
1488 757 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 478 805 1213 1624 1631 1726 2382
Size: 265 213 327 408 411 7 95 656
Cuts at: 2382 2442
Size: 60
Fragments arranged by size:
656 411 408 327 265 213 95 60
7
Bpu10I CC'TnA_GC
Cuts at: 0 197 541 1363 2098 2442
Size: 197 344 822 735 344
Fragments arranged by size:
822 735 344 344 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2167 2442
Size: 2167 275
BsaI GGTCTCn'nnnn_
Cuts at: 0 625 2442
Size: 625 1817
BsaHI Gr'CG_yC
Cuts at: 0 571 2442
Size: 571 1871
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1622 1652 2442
Size: 1622 30 790
Fragments arranged by size:
1622 790 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 1020 2041 2086 2442
Size: 103 917 1021 45 356
Fragments arranged by size:
1021 917 356 103 45
BseYI C'CCAG_C
Cuts at: 0 797 828 902 1113 1583 1616 1763 1773
Size: 797 31 74 211 470 33 147 10
Cuts at: 1773 1838 1871 2033 2442
Size: 65 33 162 409
Fragments arranged by size:
797 470 409 211 162 147 74 65
33 33 31 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 1695 2034 2146 2442
Size: 384 1311 339 112 296
Fragments arranged by size:
1311 384 339 296 112
BsiHKAI G_wGCw'C
Cuts at: 0 584 1377 1847 2442
Size: 584 793 470 595
Fragments arranged by size:
793 595 584 470
Bsp1286I G_dGCh'C
Cuts at: 0 24 32 128 137 203 416 540 584
Size: 24 8 96 9 66 213 124 44
Cuts at: 584 870 903 1191 1377 1532 1617 1780 1847
Size: 286 33 288 186 155 85 163 67
Cuts at: 1847 1962 2040 2157 2256 2344 2442
Size: 115 78 117 99 88 98
Fragments arranged by size:
288 286 213 186 163 155 124 117
115 99 98 96 88 85 78 67
66 44 33 24 9 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 412 527 710 1132 1363 2203 2363 2442
Size: 412 115 183 422 231 840 160 79
Fragments arranged by size:
840 422 412 231 183 160 115 79
BsrBI CCG'CTC
Cuts at: 0 66 2442
Size: 66 2376
BsrFI r'CCGG_y
Cuts at: 0 2336 2442
Size: 2336 106
BsrGI T'GTAC_A
Cuts at: 0 558 2442
Size: 558 1884
BssSI C'ACGA_G
Cuts at: 0 2264 2442
Size: 2264 178
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1381 2442
Size: 1381 1061
BstBI TT'CG_AA
Cuts at: 0 1232 2442
Size: 1232 1210
BstEII G'GTnAC_C
Cuts at: 0 2350 2442
Size: 2350 92
BstXI CCAn_nnnn'nTGG
Cuts at: 0 307 642 1017 1990 2442
Size: 307 335 375 973 452
Fragments arranged by size:
973 452 375 335 307
BstYI r'GATC_y
Cuts at: 0 510 1788 1947 2442
Size: 510 1278 159 495
Fragments arranged by size:
1278 510 495 159
Bsu36I CC'TnA_GG
Cuts at: 0 205 2442
Size: 205 2237
BtgI C'CryG_G
Cuts at: 0 1983 2442
Size: 1983 459
BtsI GCAGTG_nn'
Cuts at: 0 1306 1441 1594 1866 2013 2314 2442
Size: 1306 135 153 272 147 301 128
Fragments arranged by size:
1306 301 272 153 147 135 128
ClaI AT'CG_AT
Cuts at: 0 1932 2442
Size: 1932 510
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1674 1709 2442
Size: 1674 35 733
Fragments arranged by size:
1674 733 35
DraIII CAC_nnn'GTG
Cuts at: 0 1381 2442
Size: 1381 1061
DrdI GACnn_nn'nnGTC
Cuts at: 0 1400 2442
Size: 1400 1042
EaeI y'GGCC_r
Cuts at: 0 61 947 2233 2442
Size: 61 886 1286 209
Fragments arranged by size:
1286 886 209 61
EarI CTCTTCn'nnn_
Cuts at: 0 357 1234 2442
Size: 357 877 1208
Fragments arranged by size:
1208 877 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 166 265 463 478 805 1152 1213
Size: 130 36 99 198 15 327 347 61
Cuts at: 1213 1624 1631 1726 2382 2442
Size: 411 7 95 656 60
Fragments arranged by size:
656 411 347 327 198 130 99 95
61 60 36 15 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 172 460 1448 2442
Size: 172 288 988 994
Fragments arranged by size:
994 988 288 172
EcoO109I rG'GnC_Cy
Cuts at: 0 21 114 141 466 474 484 1188 1606
Size: 21 93 27 325 8 10 704 418
Cuts at: 1606 1760 2188 2239 2252 2253 2442
Size: 154 428 51 13 1 189
Fragments arranged by size:
704 428 418 325 189 154 93 51
27 21 13 10 8 1
EcoRI G'AATT_C
Cuts at: 0 1471 2442
Size: 1471 971
HaeII r_GCGC'y
Cuts at: 0 13 600 2410 2442
Size: 13 587 1810 32
Fragments arranged by size:
1810 587 32 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1752 1784 1979 2011 2442
Size: 1752 32 195 32 431
Fragments arranged by size:
1752 431 195 32 32
KpnI G_GTAC'C
Cuts at: 0 300 2151 2395 2442
Size: 300 1851 244 47
Fragments arranged by size:
1851 300 244 47
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 2442
Size: 197 2245
MscI TGG'CCA
Cuts at: 0 949 2442
Size: 949 1493
MslI CAynn'nnrTG
Cuts at: 0 316 533 896 1965 1988 2442
Size: 316 217 363 1069 23 454
Fragments arranged by size:
1069 454 363 316 217 23
MspA1I CmG'CkG
Cuts at: 0 832 906 1223 1362 1416 1624 1838 2442
Size: 832 74 317 139 54 208 214 604
Fragments arranged by size:
832 604 317 214 208 139 74 54
NcoI C'CATG_G
Cuts at: 0 1983 2442
Size: 1983 459
NruI TCG'CGA
Cuts at: 0 501 2442
Size: 501 1941
NspI r_CATG'y
Cuts at: 0 1560 2442
Size: 1560 882
PasI CC'CwG_GG
Cuts at: 0 24 588 2331 2365 2442
Size: 24 564 1743 34 77
Fragments arranged by size:
1743 564 77 34 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 1047 1059 1944 2144 2371 2442
Size: 1047 12 885 200 227 71
Fragments arranged by size:
1047 885 227 200 71 12
PfoI T'CCnGG_A
Cuts at: 0 242 1205 1316 1784 2442
Size: 242 963 111 468 658
Fragments arranged by size:
963 658 468 242 111
PpuMI rG'GwC_Cy
Cuts at: 0 141 474 484 1606 1760 2188 2239 2442
Size: 141 333 10 1122 154 428 51 203
Fragments arranged by size:
1122 428 333 203 154 141 51 10
PspOMI G'GGCC_C
Cuts at: 0 20 1187 2252 2442
Size: 20 1167 1065 190
Fragments arranged by size:
1167 1065 190 20
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 337 369 2442
Size: 337 32 2073
Fragments arranged by size:
2073 337 32
PstI C_TGCA'G
Cuts at: 0 1180 1837 2442
Size: 1180 657 605
Fragments arranged by size:
1180 657 605
PvuII CAG'CTG
Cuts at: 0 832 906 1223 1362 1416 1838 2442
Size: 832 74 317 139 54 422 604
Fragments arranged by size:
832 604 422 317 139 74 54
SanDI GG'GwC_CC
Cuts at: 0 2188 2442
Size: 2188 254
SexAI A'CCwGG_T
Cuts at: 0 806 2442
Size: 806 1636
SfcI C'TryA_G
Cuts at: 0 253 1176 1833 2442
Size: 253 923 657 609
Fragments arranged by size:
923 657 609 253
SmlI C'TyrA_G
Cuts at: 0 2182 2442
Size: 2182 260
SpeI A'CTAG_T
Cuts at: 0 774 2442
Size: 774 1668
SphI G_CATG'C
Cuts at: 0 1560 2442
Size: 1560 882
StuI AGG'CCT
Cuts at: 0 790 1138 1160 2442
Size: 790 348 22 1282
Fragments arranged by size:
1282 790 348 22
StyI C'CwwG_G
Cuts at: 0 128 1515 1983 2305 2442
Size: 128 1387 468 322 137
Fragments arranged by size:
1387 468 322 137 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 378 1830 2442
Size: 378 1452 612
Fragments arranged by size:
1452 612 378
TatI w'GTAC_w
Cuts at: 0 558 2201 2442
Size: 558 1643 241
Fragments arranged by size:
1643 558 241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 786 818 1622 1654 2442
Size: 786 32 804 32 788
Fragments arranged by size:
804 788 786 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1327 2424 2442
Size: 1327 1097 18
Fragments arranged by size:
1327 1097 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 290 910 1014 1078 2442
Size: 290 620 104 64 1364
Fragments arranged by size:
1364 620 290 104 64
Enzymes that do cut:
AarI Acc65I AcuI AhdI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI AvrII BanI BanII BbvCI BcgI
BclI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI DraIII
DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I KpnI MmeI MscI MslI MspA1I NcoI NruI
NspI PasI PflMI PfoI PpuMI PspOMI PsrI PstI
PvuII SanDI SexAI SfcI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AloI
AscI AseI AsiSI BaeI BamHI BbeI BbsI BciVI
BfrBI BlpI BmgBI BmtI BplI BsaAI BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII
BstZ17I BtgZI DraI EagI EciI EcoICRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI
SwaI XbaI XhoI XmaI XmnI ZraI