Restriction map for
pFN21AE1319
(Linear) (Six-base) MAPSORT of: KIEE1319.seq Check: 5989 from: 1 to: 939
KIEE1319.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 790 899 939
Size: 748 42 109 40
Fragments arranged by size:
748 109 42 40
AflIII A'CryG_T
Cuts at: 0 231 939
Size: 231 708
AlwNI CAG_nnn'CTG
Cuts at: 0 99 939
Size: 99 840
ApaI G_GGCC'C
Cuts at: 0 397 939
Size: 397 542
ApoI r'AATT_y
Cuts at: 0 208 797 939
Size: 208 589 142
Fragments arranged by size:
589 208 142
AvaI C'yCGr_G
Cuts at: 0 341 939
Size: 341 598
BamHI G'GATC_C
Cuts at: 0 280 337 939
Size: 280 57 602
Fragments arranged by size:
602 280 57
BanI G'GyrC_C
Cuts at: 0 154 160 939
Size: 154 6 779
Fragments arranged by size:
779 154 6
BanII G_rGCy'C
Cuts at: 0 397 939
Size: 397 542
BbsI GAAGACnn'nnnn_
Cuts at: 0 296 887 939
Size: 296 591 52
Fragments arranged by size:
591 296 52
BglI GCCn_nnn'nGGC
Cuts at: 0 30 939
Size: 30 909
BglII A'GATC_T
Cuts at: 0 555 939
Size: 555 384
Bme1580I G_kGCm'C
Cuts at: 0 157 397 939
Size: 157 240 542
Fragments arranged by size:
542 240 157
BmrI ACTGGGnnnn_n'
Cuts at: 0 391 939
Size: 391 548
Bpu10I CC'TnA_GC
Cuts at: 0 835 939
Size: 835 104
BsaAI yAC'GTr
Cuts at: 0 701 939
Size: 701 238
BsaHI Gr'CG_yC
Cuts at: 0 62 131 939
Size: 62 69 808
Fragments arranged by size:
808 69 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 104 109 134 250 280 939
Size: 79 25 5 25 116 30 659
Fragments arranged by size:
659 116 79 30 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 939
Size: 271 668
BsiEI CG_ry'CG
Cuts at: 0 147 939
Size: 147 792
Bsp1286I G_dGCh'C
Cuts at: 0 157 397 939
Size: 157 240 542
Fragments arranged by size:
542 240 157
BsrBI CCG'CTC
Cuts at: 0 57 939
Size: 57 882
BsrFI r'CCGG_y
Cuts at: 0 10 29 157 939
Size: 10 19 128 782
Fragments arranged by size:
782 128 19 10
BssHII G'CGCG_C
Cuts at: 0 187 939
Size: 187 752
BstBI TT'CG_AA
Cuts at: 0 846 939
Size: 846 93
BstYI r'GATC_y
Cuts at: 0 107 280 337 555 939
Size: 107 173 57 218 384
Fragments arranged by size:
384 218 173 107 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 663 939
Size: 663 276
EaeI y'GGCC_r
Cuts at: 0 8 939
Size: 8 931
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 65 939
Size: 65 874
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 790 899 939
Size: 748 42 109 40
Fragments arranged by size:
748 109 42 40
EcoRI G'AATT_C
Cuts at: 0 208 939
Size: 208 731
FspI TGC'GCA
Cuts at: 0 713 939
Size: 713 226
HaeII r_GCGC'y
Cuts at: 0 574 939
Size: 574 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 305 337 433 465 939
Size: 305 32 96 32 474
Fragments arranged by size:
474 305 96 32 32
HincII GTy'rAC
Cuts at: 0 474 939
Size: 474 465
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 937 939
Size: 135 802 2
Fragments arranged by size:
802 135 2
MslI CAynn'nnrTG
Cuts at: 0 703 939
Size: 703 236
MspA1I CmG'CkG
Cuts at: 0 125 633 939
Size: 125 508 306
Fragments arranged by size:
508 306 125
NaeI GCC'GGC
Cuts at: 0 12 31 939
Size: 12 19 908
Fragments arranged by size:
908 19 12
NgoMIV G'CCGG_C
Cuts at: 0 10 29 939
Size: 10 19 910
Fragments arranged by size:
910 19 10
NspI r_CATG'y
Cuts at: 0 183 939
Size: 183 756
PflMI CCAn_nnn'nTGG
Cuts at: 0 403 939
Size: 403 536
PspOMI G'GGCC_C
Cuts at: 0 393 939
Size: 393 546
PstI C_TGCA'G
Cuts at: 0 95 939
Size: 95 844
PvuII CAG'CTG
Cuts at: 0 633 939
Size: 633 306
SfcI C'TryA_G
Cuts at: 0 91 192 939
Size: 91 101 747
Fragments arranged by size:
747 101 91
SphI G_CATG'C
Cuts at: 0 183 939
Size: 183 756
StyI C'CwwG_G
Cuts at: 0 308 939
Size: 308 631
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 250 282 939
Size: 250 32 657
Fragments arranged by size:
657 250 32
XmnI GAAnn'nnTTC
Cuts at: 0 884 939
Size: 884 55
Enzymes that do cut:
AcuI AflIII AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbsI BglI BglII Bme1580I BmrI Bpu10I BsaAI
BsaHI BsaXI BseRI BsiEI Bsp1286I BsrBI BsrFI BssHII
BstBI BstYI BtgZI EaeI EciI Eco57MI EcoRI FspI
HaeII Hin4I HincII MmeI MslI MspA1I NaeI NgoMIV
NspI PflMI PspOMI PstI PvuII SfcI SphI StyI
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI
BlpI BmgBI BmtI BplI BpmI BpuEI BsaI BsaBI
BsaWI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrGI BssSI BstAPI BstEII BstXI
BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII
DrdI EagI EarI EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
MscI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI
XmaI ZraI