(Linear) (Six-base) MAPSORT of: KIEE1313.seq  Check: 2901  from: 1  to: 654



KIEE1313.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:36  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     424     654 
   Size:        382      42     230

  Fragments arranged by size: 

                 382     230      42
 
AlwNI CAG_nnn'CTG

Cuts at:      0     304     654 
   Size:        304     350
 
ApoI r'AATT_y

Cuts at:      0     129     175     574     598     654 
   Size:        129      46     399      24      56

  Fragments arranged by size: 

                 399     129      56      46      24
 
BanII G_rGCy'C

Cuts at:      0      40     298     477     487     637     654 
   Size:         40     258     179      10     150      17

  Fragments arranged by size: 

                 258     179     150      40      17      10
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      86     654 
   Size:         86     568
 
BglII A'GATC_T

Cuts at:      0     266     654 
   Size:        266     388
 
BlpI GC'TnA_GC

Cuts at:      0     297     654 
   Size:        297     357
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      57      60     632     654 
   Size:         57       3     572      22

  Fragments arranged by size: 

                 572      57      22       3
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     654 
   Size:        520     134
 
Bpu10I CC'TnA_GC

Cuts at:      0     476     654 
   Size:        476     178
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     312     654 
   Size:        312     342
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     342     654 
   Size:        342     312
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     516     654 
   Size:        516     138
 
BsiHKAI G_wGCw'C

Cuts at:      0     298     654 
   Size:        298     356
 
BsmI GAATG_Cn'

Cuts at:      0     182     654 
   Size:        182     472
 
Bsp1286I G_dGCh'C

Cuts at:      0      40     298     477     487     637     654 
   Size:         40     258     179      10     150      17

  Fragments arranged by size: 

                 258     179     150      40      17      10
 
BspHI T'CATG_A

Cuts at:      0     186     654 
   Size:        186     468
 
BsrFI r'CCGG_y

Cuts at:      0     452     654 
   Size:        452     202
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     539     654 
   Size:        539     115
 
BstYI r'GATC_y

Cuts at:      0     266     654 
   Size:        266     388
 
EcoICRI GAG'CTC

Cuts at:      0     296     654 
   Size:        296     358
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     424     520     654 
   Size:        382      42      96     134

  Fragments arranged by size: 

                 382     134      96      42
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     512     544     654 
   Size:        512      32     110

  Fragments arranged by size: 

                 512     110      32
 
MfeI C'AATT_G

Cuts at:      0     543     654 
   Size:        543     111
 
MspA1I CmG'CkG

Cuts at:      0     402     654 
   Size:        402     252
 
NaeI GCC'GGC

Cuts at:      0     454     654 
   Size:        454     200
 
NgoMIV G'CCGG_C

Cuts at:      0     452     654 
   Size:        452     202
 
NspI r_CATG'y

Cuts at:      0     498     654 
   Size:        498     156
 
PfoI T'CCnGG_A

Cuts at:      0     212     487     654 
   Size:        212     275     167

  Fragments arranged by size: 

                 275     212     167
 
PvuII CAG'CTG

Cuts at:      0     402     654 
   Size:        402     252
 
SacI G_AGCT'C

Cuts at:      0     298     654 
   Size:        298     356
 
ScaI AGT'ACT

Cuts at:      0     206     654 
   Size:        206     448
 
SmlI C'TyrA_G

Cuts at:      0     291     654 
   Size:        291     363
 
TatI w'GTAC_w

Cuts at:      0     204     654 
   Size:        204     450
 
Tth111I GACn'n_nGTC

Cuts at:      0     383     654 
   Size:        383     271
 
XmnI GAAnn'nnTTC

Cuts at:      0     175     654 
   Size:        175     479
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    BanII     BbsI    BglII     BlpI     BmrI
     BpmI   Bpu10I    BpuEI     BsaI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BsrFI   BstAPI    BstYI  EcoICRI  Eco57MI    Hin4I     MfeI
   MspA1I     NaeI   NgoMIV     NspI     PfoI    PvuII     SacI     ScaI
     SmlI     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI Bme1580I    BmgBI
     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     MslI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TstI     XbaI     XcmI     XhoI     XmaI     ZraI