Restriction map for
pFN21AE1312
(Linear) (Six-base) MAPSORT of: KIEE1312.seq Check: 843 from: 1 to: 1440
KIEE1312.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 254 1440
Size: 254 1186
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 511 585 740 1440
Size: 29 482 74 155 700
Fragments arranged by size:
700 482 155 74 29
AflII C'TTAA_G
Cuts at: 0 637 1440
Size: 637 803
AflIII A'CryG_T
Cuts at: 0 1226 1346 1440
Size: 1226 120 94
Fragments arranged by size:
1226 120 94
AlwNI CAG_nnn'CTG
Cuts at: 0 1290 1440
Size: 1290 150
ApaLI G'TGCA_C
Cuts at: 0 1176 1440
Size: 1176 264
ApoI r'AATT_y
Cuts at: 0 838 1150 1440
Size: 838 312 290
Fragments arranged by size:
838 312 290
AseI AT'TA_AT
Cuts at: 0 334 1440
Size: 334 1106
AvaI C'yCGr_G
Cuts at: 0 898 1440
Size: 898 542
AvrII C'CTAG_G
Cuts at: 0 750 1440
Size: 750 690
BamHI G'GATC_C
Cuts at: 0 585 1440
Size: 585 855
BanII G_rGCy'C
Cuts at: 0 992 1440
Size: 992 448
BbsI GAAGACnn'nnnn_
Cuts at: 0 499 1440
Size: 499 941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1157 1191 1440
Size: 1157 34 249
Fragments arranged by size:
1157 249 34
BfrBI ATG'CAT
Cuts at: 0 858 1440
Size: 858 582
BglI GCCn_nnn'nGGC
Cuts at: 0 947 1440
Size: 947 493
Bme1580I G_kGCm'C
Cuts at: 0 1180 1440
Size: 1180 260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1120 1227 1258 1440
Size: 1120 107 31 182
Fragments arranged by size:
1120 182 107 31
Bpu10I CC'TnA_GC
Cuts at: 0 250 1440
Size: 250 1190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1440
Size: 565 875
BsaI GGTCTCn'nnnn_
Cuts at: 0 232 1440
Size: 232 1208
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1200 1230 1298 1328 1440
Size: 1200 30 68 30 112
Fragments arranged by size:
1200 112 68 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 959 1440
Size: 959 481
BsiHKAI G_wGCw'C
Cuts at: 0 905 1180 1440
Size: 905 275 260
Fragments arranged by size:
905 275 260
Bsp1286I G_dGCh'C
Cuts at: 0 905 992 1180 1440
Size: 905 87 188 260
Fragments arranged by size:
905 260 188 87
BspHI T'CATG_A
Cuts at: 0 344 1440
Size: 344 1096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 206 254 1277 1440
Size: 206 48 1023 163
Fragments arranged by size:
1023 206 163 48
BsrDI GCAATG_nn'
Cuts at: 0 789 1440
Size: 789 651
BstXI CCAn_nnnn'nTGG
Cuts at: 0 877 1440
Size: 877 563
BstYI r'GATC_y
Cuts at: 0 585 1320 1440
Size: 585 735 120
Fragments arranged by size:
735 585 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1150 1440
Size: 1150 290
BtsI GCAGTG_nn'
Cuts at: 0 1279 1440
Size: 1279 161
ClaI AT'CG_AT
Cuts at: 0 770 1440
Size: 770 670
EaeI y'GGCC_r
Cuts at: 0 879 1440
Size: 879 561
EarI CTCTTCn'nnn_
Cuts at: 0 379 716 733 1440
Size: 379 337 17 707
Fragments arranged by size:
707 379 337 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 511 585 740 1120 1227 1258 1440
Size: 29 482 74 155 380 107 31 182
Fragments arranged by size:
482 380 182 155 107 74 31 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 346 1117 1440
Size: 346 771 323
Fragments arranged by size:
771 346 323
EcoO109I rG'GnC_Cy
Cuts at: 0 114 1440
Size: 114 1326
EcoRI G'AATT_C
Cuts at: 0 1150 1440
Size: 1150 290
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 52 408 1440
Size: 52 356 1032
Fragments arranged by size:
1032 356 52
HaeII r_GCGC'y
Cuts at: 0 656 1440
Size: 656 784
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 313 345 1440
Size: 313 32 1095
Fragments arranged by size:
1095 313 32
HindIII A'AGCT_T
Cuts at: 0 695 1440
Size: 695 745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 1440
Size: 392 1048
MscI TGG'CCA
Cuts at: 0 881 1440
Size: 881 559
MspA1I CmG'CkG
Cuts at: 0 1098 1440
Size: 1098 342
NsiI A_TGCA'T
Cuts at: 0 860 1440
Size: 860 580
NspI r_CATG'y
Cuts at: 0 1230 1350 1440
Size: 1230 120 90
Fragments arranged by size:
1230 120 90
PasI CC'CwG_GG
Cuts at: 0 819 1440
Size: 819 621
PciI A'CATG_T
Cuts at: 0 1226 1346 1440
Size: 1226 120 94
Fragments arranged by size:
1226 120 94
PfoI T'CCnGG_A
Cuts at: 0 1316 1440
Size: 1316 124
PpuMI rG'GwC_Cy
Cuts at: 0 114 1440
Size: 114 1326
PstI C_TGCA'G
Cuts at: 0 1274 1403 1440
Size: 1274 129 37
Fragments arranged by size:
1274 129 37
PvuII CAG'CTG
Cuts at: 0 1098 1440
Size: 1098 342
SapI GCTCTTCn'nnn_
Cuts at: 0 733 1440
Size: 733 707
SfcI C'TryA_G
Cuts at: 0 86 177 276 1270 1399 1440
Size: 86 91 99 994 129 41
Fragments arranged by size:
994 129 99 91 86 41
SmlI C'TyrA_G
Cuts at: 0 580 637 1440
Size: 580 57 803
Fragments arranged by size:
803 580 57
SspI AAT'ATT
Cuts at: 0 1387 1440
Size: 1387 53
StyI C'CwwG_G
Cuts at: 0 750 1376 1440
Size: 750 626 64
Fragments arranged by size:
750 626 64
TatI w'GTAC_w
Cuts at: 0 509 1216 1440
Size: 509 707 224
Fragments arranged by size:
707 509 224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 961 1440
Size: 961 479
XmnI GAAnn'nnTTC
Cuts at: 0 388 1440
Size: 388 1052
Enzymes that do cut:
AarI AcuI AflII AflIII AlwNI ApaLI ApoI AseI
AvaI AvrII BamHI BanII BbsI BcgI BfrBI BglI
Bme1580I BpmI Bpu10I BpuEI BsaI BsaXI BseRI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BstXI BstYI BtgZI BtsI
ClaI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HindIII MmeI MscI MspA1I NsiI NspI
PasI PciI PfoI PpuMI PstI PvuII SapI SfcI
SmlI SspI StyI TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI AscI AsiSI BaeI BanI BbeI
BbvCI BciVI BclI BglII BlpI BmgBI BmrI BmtI
BplI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoRV FseI
FspI FspAI HincII HpaI KasI KpnI MfeI MluI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI ZraI