Restriction map for
pFN21AE1308
(Linear) (Six-base) MAPSORT of: KIEE1308.seq Check: 1234 from: 1 to: 1572
KIEE1308.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 376 700 1417 1513 1572
Size: 83 293 324 717 96 59
Fragments arranged by size:
717 324 293 96 83 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 548 1572
Size: 548 1024
AlwNI CAG_nnn'CTG
Cuts at: 0 162 333 1051 1572
Size: 162 171 718 521
Fragments arranged by size:
718 521 171 162
ApaI G_GGCC'C
Cuts at: 0 13 1572
Size: 13 1559
ApoI r'AATT_y
Cuts at: 0 585 1572
Size: 585 987
BanII G_rGCy'C
Cuts at: 0 13 20 226 1448 1572
Size: 13 7 206 1222 124
Fragments arranged by size:
1222 206 124 13 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1232 1493 1572
Size: 1232 261 79
Fragments arranged by size:
1232 261 79
BciVI GTATCCnnnnn_n'
Cuts at: 0 1399 1572
Size: 1399 173
BglII A'GATC_T
Cuts at: 0 507 1572
Size: 507 1065
Bme1580I G_kGCm'C
Cuts at: 0 13 1572
Size: 13 1559
BmrI ACTGGGnnnn_n'
Cuts at: 0 16 603 1572
Size: 16 587 969
Fragments arranged by size:
969 587 16
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 717 1572
Size: 717 855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 478 783 1067 1572
Size: 208 270 305 284 505
Fragments arranged by size:
505 305 284 270 208
Bpu10I CC'TnA_GC
Cuts at: 0 1382 1572
Size: 1382 190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 1572
Size: 821 751
BsaI GGTCTCn'nnnn_
Cuts at: 0 688 816 1572
Size: 688 128 756
Fragments arranged by size:
756 688 128
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 216 375 703 706 719 856 882 1572
Size: 216 159 328 3 13 137 26 690
Fragments arranged by size:
690 328 216 159 137 26 13 3
BseYI C'CCAG_C
Cuts at: 0 537 1051 1572
Size: 537 514 521
Fragments arranged by size:
537 521 514
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1214 1572
Size: 463 751 358
Fragments arranged by size:
751 463 358
Bsp1286I G_dGCh'C
Cuts at: 0 13 20 226 1448 1572
Size: 13 7 206 1222 124
Fragments arranged by size:
1222 206 124 13 7
BspHI T'CATG_A
Cuts at: 0 737 1572
Size: 737 835
BsrFI r'CCGG_y
Cuts at: 0 1325 1457 1572
Size: 1325 132 115
Fragments arranged by size:
1325 132 115
BsrGI T'GTAC_A
Cuts at: 0 1550 1572
Size: 1550 22
BssSI C'ACGA_G
Cuts at: 0 467 702 1471 1572
Size: 467 235 769 101
Fragments arranged by size:
769 467 235 101
BstYI r'GATC_y
Cuts at: 0 507 1572
Size: 507 1065
BtsI GCAGTG_nn'
Cuts at: 0 174 579 1202 1287 1572
Size: 174 405 623 85 285
Fragments arranged by size:
623 405 285 174 85
DraI TTT'AAA
Cuts at: 0 1070 1482 1572
Size: 1070 412 90
Fragments arranged by size:
1070 412 90
EarI CTCTTCn'nnn_
Cuts at: 0 46 352 517 814 868 938 1211 1528
Size: 46 306 165 297 54 70 273 317
Cuts at: 1528 1572
Size: 44
Fragments arranged by size:
317 306 297 273 165 70 54 46
44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 208 376 478 700 783 1067 1417
Size: 83 125 168 102 222 83 284 350
Cuts at: 1417 1513 1572
Size: 96 59
Fragments arranged by size:
350 284 222 168 125 102 96 83
83 59
EcoO109I rG'GnC_Cy
Cuts at: 0 1005 1312 1572
Size: 1005 307 260
Fragments arranged by size:
1005 307 260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 307 1434 1572
Size: 307 1127 138
Fragments arranged by size:
1127 307 138
HaeII r_GCGC'y
Cuts at: 0 88 98 1572
Size: 88 10 1474
Fragments arranged by size:
1474 88 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 714 746 921 953 1169 1201 1539 1571
Size: 714 32 175 32 216 32 338 32
Cuts at: 1571 1572
Size: 1
Fragments arranged by size:
714 338 216 175 32 32 32 32
1
HincII GTy'rAC
Cuts at: 0 236 905 1572
Size: 236 669 667
Fragments arranged by size:
669 667 236
MslI CAynn'nnrTG
Cuts at: 0 485 1279 1572
Size: 485 794 293
Fragments arranged by size:
794 485 293
MspA1I CmG'CkG
Cuts at: 0 153 1572
Size: 153 1419
NdeI CA'TA_TG
Cuts at: 0 1164 1572
Size: 1164 408
NspI r_CATG'y
Cuts at: 0 882 1323 1572
Size: 882 441 249
Fragments arranged by size:
882 441 249
PfoI T'CCnGG_A
Cuts at: 0 494 1572
Size: 494 1078
PpuMI rG'GwC_Cy
Cuts at: 0 1005 1312 1572
Size: 1005 307 260
Fragments arranged by size:
1005 307 260
PspOMI G'GGCC_C
Cuts at: 0 9 1572
Size: 9 1563
PstI C_TGCA'G
Cuts at: 0 52 158 215 1572
Size: 52 106 57 1357
Fragments arranged by size:
1357 106 57 52
PvuII CAG'CTG
Cuts at: 0 153 1572
Size: 153 1419
SapI GCTCTTCn'nnn_
Cuts at: 0 46 868 1528 1572
Size: 46 822 660 44
Fragments arranged by size:
822 660 46 44
SfcI C'TryA_G
Cuts at: 0 48 154 211 1572
Size: 48 106 57 1361
Fragments arranged by size:
1361 106 57 48
SmlI C'TyrA_G
Cuts at: 0 836 1572
Size: 836 736
StyI C'CwwG_G
Cuts at: 0 1000 1025 1572
Size: 1000 25 547
Fragments arranged by size:
1000 547 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 797 1572
Size: 797 775
TatI w'GTAC_w
Cuts at: 0 859 1550 1572
Size: 859 691 22
Fragments arranged by size:
859 691 22
XbaI T'CTAG_A
Cuts at: 0 255 1572
Size: 255 1317
XmnI GAAnn'nnTTC
Cuts at: 0 315 1498 1572
Size: 315 1183 74
Fragments arranged by size:
1183 315 74
Enzymes that do cut:
AcuI AlfI AlwNI ApaI ApoI BanII BbsI BciVI
BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BseRI BseYI BsgI Bsp1286I BspHI BsrFI BsrGI BssSI
BstYI BtsI DraI EarI Eco57MI EcoO109I FalI HaeII
Hin4I HincII MslI MspA1I NdeI NspI PfoI PpuMI
PspOMI PstI PvuII SapI SfcI SmlI StyI TaqII
TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BclI BfrBI BglI BlpI BmgBI BmtI BsaAI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrDI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XcmI XhoI XmaI
ZraI