Restriction map for
pFN21AE1307
(Linear) (Six-base) MAPSORT of: KIEE1307.seq Check: 8539 from: 1 to: 1416
KIEE1307.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 244 1416
Size: 244 1172
AatII G_ACGT'C
Cuts at: 0 464 1416
Size: 464 952
AccI GT'mk_AC
Cuts at: 0 80 1286 1416
Size: 80 1206 130
Fragments arranged by size:
1206 130 80
Acc65I G'GTAC_C
Cuts at: 0 572 1416
Size: 572 844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 954 1327 1416
Size: 223 731 373 89
Fragments arranged by size:
731 373 223 89
AfeI AGC'GCT
Cuts at: 0 826 1416
Size: 826 590
AhdI GACnn_n'nnGTC
Cuts at: 0 153 589 1416
Size: 153 436 827
Fragments arranged by size:
827 436 153
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 997 1416
Size: 997 419
AlwNI CAG_nnn'CTG
Cuts at: 0 112 245 419 723 781 977 1223 1416
Size: 112 133 174 304 58 196 246 193
Fragments arranged by size:
304 246 196 193 174 133 112 58
ApaI G_GGCC'C
Cuts at: 0 345 1416
Size: 345 1071
AvaI C'yCGr_G
Cuts at: 0 338 1416
Size: 338 1078
BanI G'GyrC_C
Cuts at: 0 572 876 1416
Size: 572 304 540
Fragments arranged by size:
572 540 304
BanII G_rGCy'C
Cuts at: 0 331 338 345 371 1124 1349 1376 1416
Size: 331 7 7 26 753 225 27 40
Fragments arranged by size:
753 331 225 40 27 26 7 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 231 265 1416
Size: 231 34 1151
Fragments arranged by size:
1151 231 34
BclI T'GATC_A
Cuts at: 0 443 596 926 1416
Size: 443 153 330 490
Fragments arranged by size:
490 443 330 153
BlpI GC'TnA_GC
Cuts at: 0 1174 1416
Size: 1174 242
Bme1580I G_kGCm'C
Cuts at: 0 345 881 1416
Size: 345 536 535
Fragments arranged by size:
536 535 345
BmgBI CAC'GTC
Cuts at: 0 324 1208 1416
Size: 324 884 208
Fragments arranged by size:
884 324 208
BmrI ACTGGGnnnn_n'
Cuts at: 0 156 284 1416
Size: 156 128 1132
Fragments arranged by size:
1132 156 128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1111 1172 1416
Size: 67 1044 61 244
Fragments arranged by size:
1044 244 67 61
BsaI GGTCTCn'nnnn_
Cuts at: 0 528 1017 1416
Size: 528 489 399
Fragments arranged by size:
528 489 399
BsaHI Gr'CG_yC
Cuts at: 0 461 1416
Size: 461 955
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1064 1094 1416
Size: 1064 30 322
Fragments arranged by size:
1064 322 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 291 1416
Size: 291 1125
BseYI C'CCAG_C
Cuts at: 0 1112 1356 1416
Size: 1112 244 60
Fragments arranged by size:
1112 244 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 533 736 751 1416
Size: 337 196 203 15 665
Fragments arranged by size:
665 337 203 196 15
BsiEI CG_ry'CG
Cuts at: 0 957 1416
Size: 957 459
BsiHKAI G_wGCw'C
Cuts at: 0 1376 1416
Size: 1376 40
BsmBI CGTCTCn'nnnn_
Cuts at: 0 452 499 1416
Size: 452 47 917
Fragments arranged by size:
917 452 47
Bsp1286I G_dGCh'C
Cuts at: 0 331 338 345 371 881 1124 1349 1376
Size: 331 7 7 26 510 243 225 27
Cuts at: 1376 1416
Size: 40
Fragments arranged by size:
510 331 243 225 40 27 26 7
7
BspHI T'CATG_A
Cuts at: 0 593 1416
Size: 593 823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 92 244 1266 1416
Size: 92 152 1022 150
Fragments arranged by size:
1022 152 150 92
BsrDI GCAATG_nn'
Cuts at: 0 477 1416
Size: 477 939
BsrFI r'CCGG_y
Cuts at: 0 182 1416
Size: 182 1234
BsrGI T'GTAC_A
Cuts at: 0 1214 1416
Size: 1214 202
BstAPI GCAn_nnn'nTGC
Cuts at: 0 245 1416
Size: 245 1171
BstXI CCAn_nnnn'nTGG
Cuts at: 0 45 1326 1416
Size: 45 1281 90
Fragments arranged by size:
1281 90 45
BstYI r'GATC_y
Cuts at: 0 755 1416
Size: 755 661
Bsu36I CC'TnA_GG
Cuts at: 0 994 1416
Size: 994 422
BtsI GCAGTG_nn'
Cuts at: 0 417 1416
Size: 417 999
DraIII CAC_nnn'GTG
Cuts at: 0 608 1416
Size: 608 808
EaeI y'GGCC_r
Cuts at: 0 131 425 1416
Size: 131 294 991
Fragments arranged by size:
991 294 131
EarI CTCTTCn'nnn_
Cuts at: 0 65 1051 1416
Size: 65 986 365
Fragments arranged by size:
986 365 65
EcoICRI GAG'CTC
Cuts at: 0 1374 1416
Size: 1374 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 223 954 1111 1172 1327 1416
Size: 67 156 731 157 61 155 89
Fragments arranged by size:
731 157 156 155 89 67 61
EcoO109I rG'GnC_Cy
Cuts at: 0 61 117 342 990 1075 1156 1416
Size: 61 56 225 648 85 81 260
Fragments arranged by size:
648 260 225 85 81 61 56
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 757 1416
Size: 757 659
FspI TGC'GCA
Cuts at: 0 804 1416
Size: 804 612
HaeII r_GCGC'y
Cuts at: 0 705 828 1194 1416
Size: 705 123 366 222
Fragments arranged by size:
705 366 222 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 313 345 575 607 711 743
Size: 139 32 142 32 230 32 104 32
Cuts at: 743 1016 1048 1384 1416
Size: 273 32 336 32
Fragments arranged by size:
336 273 230 142 139 104 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 0 147 1416
Size: 147 1269
KpnI G_GTAC'C
Cuts at: 0 576 1416
Size: 576 840
MscI TGG'CCA
Cuts at: 0 133 427 1416
Size: 133 294 989
Fragments arranged by size:
989 294 133
MslI CAynn'nnrTG
Cuts at: 0 313 683 1416
Size: 313 370 733
Fragments arranged by size:
733 370 313
MspA1I CmG'CkG
Cuts at: 0 245 431 649 1416
Size: 245 186 218 767
Fragments arranged by size:
767 245 218 186
PasI CC'CwG_GG
Cuts at: 0 65 453 1384 1416
Size: 65 388 931 32
Fragments arranged by size:
931 388 65 32
PflMI CCAn_nnn'nTGG
Cuts at: 0 423 1416
Size: 423 993
PpuMI rG'GwC_Cy
Cuts at: 0 61 990 1416
Size: 61 929 426
Fragments arranged by size:
929 426 61
PspOMI G'GGCC_C
Cuts at: 0 341 1416
Size: 341 1075
PstI C_TGCA'G
Cuts at: 0 241 1277 1416
Size: 241 1036 139
Fragments arranged by size:
1036 241 139
PvuII CAG'CTG
Cuts at: 0 245 1416
Size: 245 1171
SacI G_AGCT'C
Cuts at: 0 1376 1416
Size: 1376 40
SapI GCTCTTCn'nnn_
Cuts at: 0 1051 1416
Size: 1051 365
SfcI C'TryA_G
Cuts at: 0 237 1273 1416
Size: 237 1036 143
Fragments arranged by size:
1036 237 143
SmaI CCC'GGG
Cuts at: 0 340 1416
Size: 340 1076
StuI AGG'CCT
Cuts at: 0 923 1416
Size: 923 493
StyI C'CwwG_G
Cuts at: 0 371 749 786 1350 1416
Size: 371 378 37 564 66
Fragments arranged by size:
564 378 371 66 37
TatI w'GTAC_w
Cuts at: 0 1214 1416
Size: 1214 202
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1064 1096 1130 1162 1416
Size: 1064 32 34 32 254
Fragments arranged by size:
1064 254 34 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 723 1416
Size: 723 693
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 393 420 1326 1416
Size: 393 27 906 90
Fragments arranged by size:
906 393 90 27
XmaI C'CCGG_G
Cuts at: 0 338 1416
Size: 338 1078
ZraI GAC'GTC
Cuts at: 0 462 1416
Size: 462 954
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AhdI AlfI
AlwNI ApaI AvaI BanI BanII BcgI BclI BlpI
Bme1580I BmgBI BmrI BpmI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoO109I FalI FspI
HaeII Hin4I HincII KpnI MscI MslI MspA1I PasI
PflMI PpuMI PspOMI PstI PvuII SacI SapI SfcI
SmaI StuI StyI TatI TstI Tth111I XcmI XmaI
ZraI
Enzymes that do not cut:
AclI AflII AflIII AgeI AleI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BbvCI BciVI BfrBI BglI BglII BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BsrBI
BssHII BssSI BstBI BstEII BstZ17I BtgI BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoNI EcoRI EcoRV
FseI FspAI HindIII HpaI KasI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII
XbaI XhoI XmnI