(Linear) (Six-base) MAPSORT of: KIEE1307.seq Check: 8539 from: 1 to: 1416 KIEE1307.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 244 1416 Size: 244 1172 AatII G_ACGT'C Cuts at: 0 464 1416 Size: 464 952 AccI GT'mk_AC Cuts at: 0 80 1286 1416 Size: 80 1206 130 Fragments arranged by size: 1206 130 80 Acc65I G'GTAC_C Cuts at: 0 572 1416 Size: 572 844 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 223 954 1327 1416 Size: 223 731 373 89 Fragments arranged by size: 731 373 223 89 AfeI AGC'GCT Cuts at: 0 826 1416 Size: 826 590 AhdI GACnn_n'nnGTC Cuts at: 0 153 589 1416 Size: 153 436 827 Fragments arranged by size: 827 436 153 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 997 1416 Size: 997 419 AlwNI CAG_nnn'CTG Cuts at: 0 112 245 419 723 781 977 1223 1416 Size: 112 133 174 304 58 196 246 193 Fragments arranged by size: 304 246 196 193 174 133 112 58 ApaI G_GGCC'C Cuts at: 0 345 1416 Size: 345 1071 AvaI C'yCGr_G Cuts at: 0 338 1416 Size: 338 1078 BanI G'GyrC_C Cuts at: 0 572 876 1416 Size: 572 304 540 Fragments arranged by size: 572 540 304 BanII G_rGCy'C Cuts at: 0 331 338 345 371 1124 1349 1376 1416 Size: 331 7 7 26 753 225 27 40 Fragments arranged by size: 753 331 225 40 27 26 7 7 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 231 265 1416 Size: 231 34 1151 Fragments arranged by size: 1151 231 34 BclI T'GATC_A Cuts at: 0 443 596 926 1416 Size: 443 153 330 490 Fragments arranged by size: 490 443 330 153 BlpI GC'TnA_GC Cuts at: 0 1174 1416 Size: 1174 242 Bme1580I G_kGCm'C Cuts at: 0 345 881 1416 Size: 345 536 535 Fragments arranged by size: 536 535 345 BmgBI CAC'GTC Cuts at: 0 324 1208 1416 Size: 324 884 208 Fragments arranged by size: 884 324 208 BmrI ACTGGGnnnn_n' Cuts at: 0 156 284 1416 Size: 156 128 1132 Fragments arranged by size: 1132 156 128 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 1111 1172 1416 Size: 67 1044 61 244 Fragments arranged by size: 1044 244 67 61 BsaI GGTCTCn'nnnn_ Cuts at: 0 528 1017 1416 Size: 528 489 399 Fragments arranged by size: 528 489 399 BsaHI Gr'CG_yC Cuts at: 0 461 1416 Size: 461 955 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1064 1094 1416 Size: 1064 30 322 Fragments arranged by size: 1064 322 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 291 1416 Size: 291 1125 BseYI C'CCAG_C Cuts at: 0 1112 1356 1416 Size: 1112 244 60 Fragments arranged by size: 1112 244 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 533 736 751 1416 Size: 337 196 203 15 665 Fragments arranged by size: 665 337 203 196 15 BsiEI CG_ry'CG Cuts at: 0 957 1416 Size: 957 459 BsiHKAI G_wGCw'C Cuts at: 0 1376 1416 Size: 1376 40 BsmBI CGTCTCn'nnnn_ Cuts at: 0 452 499 1416 Size: 452 47 917 Fragments arranged by size: 917 452 47 Bsp1286I G_dGCh'C Cuts at: 0 331 338 345 371 881 1124 1349 1376 Size: 331 7 7 26 510 243 225 27 Cuts at: 1376 1416 Size: 40 Fragments arranged by size: 510 331 243 225 40 27 26 7 7 BspHI T'CATG_A Cuts at: 0 593 1416 Size: 593 823 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 92 244 1266 1416 Size: 92 152 1022 150 Fragments arranged by size: 1022 152 150 92 BsrDI GCAATG_nn' Cuts at: 0 477 1416 Size: 477 939 BsrFI r'CCGG_y Cuts at: 0 182 1416 Size: 182 1234 BsrGI T'GTAC_A Cuts at: 0 1214 1416 Size: 1214 202 BstAPI GCAn_nnn'nTGC Cuts at: 0 245 1416 Size: 245 1171 BstXI CCAn_nnnn'nTGG Cuts at: 0 45 1326 1416 Size: 45 1281 90 Fragments arranged by size: 1281 90 45 BstYI r'GATC_y Cuts at: 0 755 1416 Size: 755 661 Bsu36I CC'TnA_GG Cuts at: 0 994 1416 Size: 994 422 BtsI GCAGTG_nn' Cuts at: 0 417 1416 Size: 417 999 DraIII CAC_nnn'GTG Cuts at: 0 608 1416 Size: 608 808 EaeI y'GGCC_r Cuts at: 0 131 425 1416 Size: 131 294 991 Fragments arranged by size: 991 294 131 EarI CTCTTCn'nnn_ Cuts at: 0 65 1051 1416 Size: 65 986 365 Fragments arranged by size: 986 365 65 EcoICRI GAG'CTC Cuts at: 0 1374 1416 Size: 1374 42 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 223 954 1111 1172 1327 1416 Size: 67 156 731 157 61 155 89 Fragments arranged by size: 731 157 156 155 89 67 61 EcoO109I rG'GnC_Cy Cuts at: 0 61 117 342 990 1075 1156 1416 Size: 61 56 225 648 85 81 260 Fragments arranged by size: 648 260 225 85 81 61 56 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 757 1416 Size: 757 659 FspI TGC'GCA Cuts at: 0 804 1416 Size: 804 612 HaeII r_GCGC'y Cuts at: 0 705 828 1194 1416 Size: 705 123 366 222 Fragments arranged by size: 705 366 222 123 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 139 171 313 345 575 607 711 743 Size: 139 32 142 32 230 32 104 32 Cuts at: 743 1016 1048 1384 1416 Size: 273 32 336 32 Fragments arranged by size: 336 273 230 142 139 104 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 147 1416 Size: 147 1269 KpnI G_GTAC'C Cuts at: 0 576 1416 Size: 576 840 MscI TGG'CCA Cuts at: 0 133 427 1416 Size: 133 294 989 Fragments arranged by size: 989 294 133 MslI CAynn'nnrTG Cuts at: 0 313 683 1416 Size: 313 370 733 Fragments arranged by size: 733 370 313 MspA1I CmG'CkG Cuts at: 0 245 431 649 1416 Size: 245 186 218 767 Fragments arranged by size: 767 245 218 186 PasI CC'CwG_GG Cuts at: 0 65 453 1384 1416 Size: 65 388 931 32 Fragments arranged by size: 931 388 65 32 PflMI CCAn_nnn'nTGG Cuts at: 0 423 1416 Size: 423 993 PpuMI rG'GwC_Cy Cuts at: 0 61 990 1416 Size: 61 929 426 Fragments arranged by size: 929 426 61 PspOMI G'GGCC_C Cuts at: 0 341 1416 Size: 341 1075 PstI C_TGCA'G Cuts at: 0 241 1277 1416 Size: 241 1036 139 Fragments arranged by size: 1036 241 139 PvuII CAG'CTG Cuts at: 0 245 1416 Size: 245 1171 SacI G_AGCT'C Cuts at: 0 1376 1416 Size: 1376 40 SapI GCTCTTCn'nnn_ Cuts at: 0 1051 1416 Size: 1051 365 SfcI C'TryA_G Cuts at: 0 237 1273 1416 Size: 237 1036 143 Fragments arranged by size: 1036 237 143 SmaI CCC'GGG Cuts at: 0 340 1416 Size: 340 1076 StuI AGG'CCT Cuts at: 0 923 1416 Size: 923 493 StyI C'CwwG_G Cuts at: 0 371 749 786 1350 1416 Size: 371 378 37 564 66 Fragments arranged by size: 564 378 371 66 37 TatI w'GTAC_w Cuts at: 0 1214 1416 Size: 1214 202 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1064 1096 1130 1162 1416 Size: 1064 32 34 32 254 Fragments arranged by size: 1064 254 34 32 32 Tth111I GACn'n_nGTC Cuts at: 0 723 1416 Size: 723 693 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 393 420 1326 1416 Size: 393 27 906 90 Fragments arranged by size: 906 393 90 27 XmaI C'CCGG_G Cuts at: 0 338 1416 Size: 338 1078 ZraI GAC'GTC Cuts at: 0 462 1416 Size: 462 954 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AhdI AlfI AlwNI ApaI AvaI BanI BanII BcgI BclI BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I HincII KpnI MscI MslI MspA1I PasI PflMI PpuMI PspOMI PstI PvuII SacI SapI SfcI SmaI StuI StyI TatI TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AclI AflII AflIII AgeI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BfrBI BglI BglII BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BsrBI BssHII BssSI BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspAI HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII XbaI XhoI XmnI