Restriction map for
pFN21AE1306
(Linear) (Six-base) MAPSORT of: KIEE1306.seq Check: 3413 from: 1 to: 1233
KIEE1306.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AatII G_ACGT'C
Cuts at: 0 755 953 1233
Size: 755 198 280
Fragments arranged by size:
755 280 198
Acc65I G'GTAC_C
Cuts at: 0 485 1233
Size: 485 748
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 681 1233
Size: 681 552
AflIII A'CryG_T
Cuts at: 0 641 1233
Size: 641 592
AlwNI CAG_nnn'CTG
Cuts at: 0 129 1233
Size: 129 1104
AvaI C'yCGr_G
Cuts at: 0 1087 1233
Size: 1087 146
BanI G'GyrC_C
Cuts at: 0 485 1233
Size: 485 748
BanII G_rGCy'C
Cuts at: 0 560 687 1100 1187 1233
Size: 560 127 413 87 46
Fragments arranged by size:
560 413 127 87 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 1038 1233
Size: 1038 195
BbvCI CC'TCA_GC
Cuts at: 0 211 1039 1233
Size: 211 828 194
Fragments arranged by size:
828 211 194
BciVI GTATCCnnnnn_n'
Cuts at: 0 971 1233
Size: 971 262
BglI GCCn_nnn'nGGC
Cuts at: 0 413 1233
Size: 413 820
BlpI GC'TnA_GC
Cuts at: 0 977 1233
Size: 977 256
Bme1580I G_kGCm'C
Cuts at: 0 107 465 1233
Size: 107 358 768
Fragments arranged by size:
768 358 107
BmgBI CAC'GTC
Cuts at: 0 309 1233
Size: 309 924
BmrI ACTGGGnnnn_n'
Cuts at: 0 1188 1233
Size: 1188 45
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 969 1233
Size: 969 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 964 1233
Size: 316 648 269
Fragments arranged by size:
648 316 269
Bpu10I CC'TnA_GC
Cuts at: 0 211 985 1039 1233
Size: 211 774 54 194
Fragments arranged by size:
774 211 194 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 334 1233
Size: 334 899
BsaAI yAC'GTr
Cuts at: 0 642 1233
Size: 642 591
BsaHI Gr'CG_yC
Cuts at: 0 278 452 752 950 1233
Size: 278 174 300 198 283
Fragments arranged by size:
300 283 278 198 174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1068 1098 1132 1162 1233
Size: 1068 30 34 30 71
Fragments arranged by size:
1068 71 34 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 696 1105 1108 1140 1233
Size: 696 409 3 32 93
Fragments arranged by size:
696 409 93 32 3
BseYI C'CCAG_C
Cuts at: 0 474 1116 1233
Size: 474 642 117
Fragments arranged by size:
642 474 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 804 1233
Size: 24 780 429
Fragments arranged by size:
780 429 24
BsmI GAATG_Cn'
Cuts at: 0 371 1233
Size: 371 862
BsmBI CGTCTCn'nnnn_
Cuts at: 0 957 1233
Size: 957 276
Bsp1286I G_dGCh'C
Cuts at: 0 107 465 560 687 1100 1187 1233
Size: 107 358 95 127 413 87 46
Fragments arranged by size:
413 358 127 107 95 87 46
BspHI T'CATG_A
Cuts at: 0 77 1233
Size: 77 1156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1043 1233
Size: 1043 190
BsrBI CCG'CTC
Cuts at: 0 8 1233
Size: 8 1225
BsrFI r'CCGG_y
Cuts at: 0 146 184 217 367 731 1233
Size: 146 38 33 150 364 502
Fragments arranged by size:
502 364 150 146 38 33
BsrGI T'GTAC_A
Cuts at: 0 248 1233
Size: 248 985
BstXI CCAn_nnnn'nTGG
Cuts at: 0 894 1107 1120 1233
Size: 894 213 13 113
Fragments arranged by size:
894 213 113 13
Bsu36I CC'TnA_GG
Cuts at: 0 757 935 1233
Size: 757 178 298
Fragments arranged by size:
757 298 178
BtgI C'CryG_G
Cuts at: 0 893 1233
Size: 893 340
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1156 1233
Size: 1156 77
BtsI GCAGTG_nn'
Cuts at: 0 41 369 1233
Size: 41 328 864
Fragments arranged by size:
864 328 41
DrdI GACnn_nn'nnGTC
Cuts at: 0 91 1233
Size: 91 1142
EaeI y'GGCC_r
Cuts at: 0 32 182 401 426 650 848 1233
Size: 32 150 219 25 224 198 385
Fragments arranged by size:
385 224 219 198 150 32 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1021 1233
Size: 1021 212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 681 964 1233
Size: 316 365 283 269
Fragments arranged by size:
365 316 283 269
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1078 1233
Size: 1078 155
EcoO109I rG'GnC_Cy
Cuts at: 0 621 930 1133 1233
Size: 621 309 203 100
Fragments arranged by size:
621 309 203 100
FspI TGC'GCA
Cuts at: 0 726 1233
Size: 726 507
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 674 706 1233
Size: 674 32 527
Fragments arranged by size:
674 527 32
KpnI G_GTAC'C
Cuts at: 0 489 1233
Size: 489 744
MscI TGG'CCA
Cuts at: 0 403 652 1233
Size: 403 249 581
Fragments arranged by size:
581 403 249
MspA1I CmG'CkG
Cuts at: 0 126 474 506 675 1107 1116 1233
Size: 126 348 32 169 432 9 117
Fragments arranged by size:
432 348 169 126 117 32 9
NaeI GCC'GGC
Cuts at: 0 186 219 369 733 1233
Size: 186 33 150 364 500
Fragments arranged by size:
500 364 186 150 33
NgoMIV G'CCGG_C
Cuts at: 0 184 217 367 731 1233
Size: 184 33 150 364 502
Fragments arranged by size:
502 364 184 150 33
NspI r_CATG'y
Cuts at: 0 324 483 1233
Size: 324 159 750
Fragments arranged by size:
750 324 159
PasI CC'CwG_GG
Cuts at: 0 518 1233
Size: 518 715
PfoI T'CCnGG_A
Cuts at: 0 386 1233
Size: 386 847
PmlI CAC'GTG
Cuts at: 0 642 1233
Size: 642 591
PpuMI rG'GwC_Cy
Cuts at: 0 621 930 1133 1233
Size: 621 309 203 100
Fragments arranged by size:
621 309 203 100
PstI C_TGCA'G
Cuts at: 0 48 155 674 1132 1233
Size: 48 107 519 458 101
Fragments arranged by size:
519 458 107 101 48
PvuII CAG'CTG
Cuts at: 0 126 474 506 675 1116 1233
Size: 126 348 32 169 441 117
Fragments arranged by size:
441 348 169 126 117 32
SanDI GG'GwC_CC
Cuts at: 0 930 1233
Size: 930 303
SbfI CC_TGCA'GG
Cuts at: 0 1132 1233
Size: 1132 101
SfcI C'TryA_G
Cuts at: 0 44 151 670 714 1128 1233
Size: 44 107 519 44 414 105
Fragments arranged by size:
519 414 107 105 44 44
SphI G_CATG'C
Cuts at: 0 483 1233
Size: 483 750
StuI AGG'CCT
Cuts at: 0 382 1233
Size: 382 851
StyI C'CwwG_G
Cuts at: 0 179 567 925 1233
Size: 179 388 358 308
Fragments arranged by size:
388 358 308 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 280 514 1108 1233
Size: 280 234 594 125
Fragments arranged by size:
594 280 234 125
TatI w'GTAC_w
Cuts at: 0 248 1233
Size: 248 985
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1068 1100 1233
Size: 1068 32 133
Fragments arranged by size:
1068 133 32
Tth111I GACn'n_nGTC
Cuts at: 0 310 751 1233
Size: 310 441 482
Fragments arranged by size:
482 441 310
ZraI GAC'GTC
Cuts at: 0 753 951 1233
Size: 753 198 282
Fragments arranged by size:
753 282 198
Enzymes that do cut:
AatII Acc65I AcuI AflIII AlwNI AvaI BanI BanII
BbsI BbvCI BciVI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrGI BstXI Bsu36I BtgI BtgZI BtsI DrdI
EaeI EciI Eco57MI EcoNI EcoO109I FspI Hin4I KpnI
MscI MspA1I NaeI NgoMIV NspI PasI PfoI PmlI
PpuMI PstI PvuII SanDI SbfI SfcI SphI StuI
StyI TaqII TatI TstI Tth111I ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BclI BfrBI BglII
BmtI BpuEI BsaBI BsaWI BsiEI BsiHKAI BsiWI BspEI
BsrDI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
ClaI CspCI DraI DraIII EagI EarI EcoICRI EcoRI
EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI MmeI MslI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
SwaI XbaI XcmI XhoI XmaI XmnI