(Linear) (Six-base) MAPSORT of: KIEE1302.seq  Check: 7448  from: 1  to: 435



KIEE1302.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:35  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     376     435 
   Size:        241     135      59

  Fragments arranged by size: 

                 241     135      59
 
AleI CACnn'nnGTG

Cuts at:      0      15     435 
   Size:         15     420
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     341     435 
   Size:        341      94
 
AlwNI CAG_nnn'CTG

Cuts at:      0     321     435 
   Size:        321     114
 
BamHI G'GATC_C

Cuts at:      0      62     435 
   Size:         62     373
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     229     435 
   Size:        229     206
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     107     435 
   Size:        107     328
 
BsaBI GATnn'nnATC

Cuts at:      0      49     435 
   Size:         49     386
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     255     435 
   Size:        255     180
 
BstYI r'GATC_y

Cuts at:      0      62     435 
   Size:         62     373
 
BtgI C'CryG_G

Cuts at:      0     176     435 
   Size:        176     259
 
BtsI GCAGTG_nn'

Cuts at:      0      10     138     285     408     435 
   Size:         10     128     147     123      27

  Fragments arranged by size: 

                 147     128     123      27      10
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     376     435 
   Size:        241     135      59

  Fragments arranged by size: 

                 241     135      59
 
MslI CAynn'nnrTG

Cuts at:      0      15     435 
   Size:         15     420
 
MspA1I CmG'CkG

Cuts at:      0     321     435 
   Size:        321     114
 
PasI CC'CwG_GG

Cuts at:      0     228     273     427     435 
   Size:        228      45     154       8

  Fragments arranged by size: 

                 228     154      45       8
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     182     435 
   Size:        182     253
 
PvuII CAG'CTG

Cuts at:      0     321     435 
   Size:        321     114
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     419     435 
   Size:        419      16
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI    BamHI     BbsI     BmrI    BsaBI
     BsgI    BstYI     BtgI     BtsI  Eco57MI     MslI   MspA1I     PasI
    PflMI    PvuII    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI