Restriction map for
pFN21AE1299
(Linear) (Six-base) MAPSORT of: KIEE1299.seq Check: 6536 from: 1 to: 765
KIEE1299.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 374 765
Size: 374 391
AlwNI CAG_nnn'CTG
Cuts at: 0 652 765
Size: 652 113
ApaI G_GGCC'C
Cuts at: 0 33 761 765
Size: 33 728 4
Fragments arranged by size:
728 33 4
ApaLI G'TGCA_C
Cuts at: 0 251 765
Size: 251 514
ApoI r'AATT_y
Cuts at: 0 151 226 765
Size: 151 75 539
Fragments arranged by size:
539 151 75
BanII G_rGCy'C
Cuts at: 0 33 58 644 761 765
Size: 33 25 586 117 4
Fragments arranged by size:
586 117 33 25 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 77 702 765
Size: 77 625 63
Fragments arranged by size:
625 77 63
BclI T'GATC_A
Cuts at: 0 501 765
Size: 501 264
BglI GCCn_nnn'nGGC
Cuts at: 0 689 765
Size: 689 76
BlpI GC'TnA_GC
Cuts at: 0 442 765
Size: 442 323
Bme1580I G_kGCm'C
Cuts at: 0 33 255 455 666 706 761 765
Size: 33 222 200 211 40 55 4
Fragments arranged by size:
222 211 200 55 40 33 4
BmrI ACTGGGnnnn_n'
Cuts at: 0 382 588 618 765
Size: 382 206 30 147
Fragments arranged by size:
382 206 147 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 765
Size: 205 560
BsaI GGTCTCn'nnnn_
Cuts at: 0 472 765
Size: 472 293
BsaAI yAC'GTr
Cuts at: 0 177 765
Size: 177 588
BseYI C'CCAG_C
Cuts at: 0 24 765
Size: 24 741
BsiHKAI G_wGCw'C
Cuts at: 0 255 578 644 765
Size: 255 323 66 121
Fragments arranged by size:
323 255 121 66
Bsp1286I G_dGCh'C
Cuts at: 0 33 58 255 455 578 644 666 706
Size: 33 25 197 200 123 66 22 40
Cuts at: 706 761 765
Size: 55 4
Fragments arranged by size:
200 197 123 66 55 40 33 25
22 4
BspHI T'CATG_A
Cuts at: 0 293 765
Size: 293 472
BsrDI GCAATG_nn'
Cuts at: 0 453 765
Size: 453 312
BssSI C'ACGA_G
Cuts at: 0 685 765
Size: 685 80
Bsu36I CC'TnA_GG
Cuts at: 0 338 765
Size: 338 427
EaeI y'GGCC_r
Cuts at: 0 135 765
Size: 135 630
EarI CTCTTCn'nnn_
Cuts at: 0 602 765
Size: 602 163
EcoICRI GAG'CTC
Cuts at: 0 642 765
Size: 642 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 765
Size: 205 560
EcoO109I rG'GnC_Cy
Cuts at: 0 29 30 582 757 758 765
Size: 29 1 552 175 1 7
Fragments arranged by size:
552 175 29 7 1 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 360 392 765
Size: 360 32 373
Fragments arranged by size:
373 360 32
HincII GTy'rAC
Cuts at: 0 429 765
Size: 429 336
MscI TGG'CCA
Cuts at: 0 137 765
Size: 137 628
MslI CAynn'nnrTG
Cuts at: 0 49 268 697 765
Size: 49 219 429 68
Fragments arranged by size:
429 219 68 49
MspA1I CmG'CkG
Cuts at: 0 319 765
Size: 319 446
PasI CC'CwG_GG
Cuts at: 0 666 765
Size: 666 99
PshAI GACnn'nnGTC
Cuts at: 0 655 765
Size: 655 110
PspOMI G'GGCC_C
Cuts at: 0 29 757 765
Size: 29 728 8
Fragments arranged by size:
728 29 8
SacI G_AGCT'C
Cuts at: 0 644 765
Size: 644 121
StuI AGG'CCT
Cuts at: 0 725 765
Size: 725 40
StyI C'CwwG_G
Cuts at: 0 720 753 765
Size: 720 33 12
Fragments arranged by size:
720 33 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 160 765
Size: 160 605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 154 765
Size: 154 611
Enzymes that do cut:
AhdI AlwNI ApaI ApaLI ApoI BanII BbsI BclI
BglI BlpI Bme1580I BmrI BpmI BsaI BsaAI BseYI
BsiHKAI Bsp1286I BspHI BsrDI BssSI Bsu36I EaeI EarI
EcoICRI Eco57MI EcoO109I Hin4I HincII MscI MslI MspA1I
PasI PshAI PspOMI SacI StuI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BglII BmgBI BmtI BplI Bpu10I BpuEI
BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI