(Linear) (Six-base) MAPSORT of: KIEE1297.seq  Check: 937  from: 1  to: 378



KIEE1297.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:32  ..

AccI GT'mk_AC

Cuts at:      0     119     378 
   Size:        119     259
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     378 
   Size:        157     221
 
AfeI AGC'GCT

Cuts at:      0     259     372     378 
   Size:        259     113       6

  Fragments arranged by size: 

                 259     113       6
 
AflIII A'CryG_T

Cuts at:      0     122     378 
   Size:        122     256
 
AvaI C'yCGr_G

Cuts at:      0     310     378 
   Size:        310      68
 
BanI G'GyrC_C

Cuts at:      0     343     378 
   Size:        343      35
 
BanII G_rGCy'C

Cuts at:      0      43     378 
   Size:         43     335
 
Bme1580I G_kGCm'C

Cuts at:      0     346     378 
   Size:        346      32
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     129     378 
   Size:        129     249
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258     378 
   Size:        258     120
 
BsaAI yAC'GTr

Cuts at:      0     123     378 
   Size:        123     255
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     156     378 
   Size:        156     222
 
BsiEI CG_ry'CG

Cuts at:      0     293     378 
   Size:        293      85
 
Bsp1286I G_dGCh'C

Cuts at:      0      43     346     378 
   Size:         43     303      32

  Fragments arranged by size: 

                 303      43      32
 
BspHI T'CATG_A

Cuts at:      0     185     378 
   Size:        185     193
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     132     378 
   Size:        132     246
 
BsrBI CCG'CTC

Cuts at:      0      22     378 
   Size:         22     356
 
BsrFI r'CCGG_y

Cuts at:      0     157     378 
   Size:        157     221
 
BsrGI T'GTAC_A

Cuts at:      0     125     378 
   Size:        125     253
 
BstBI TT'CG_AA

Cuts at:      0     212     378 
   Size:        212     166
 
BstYI r'GATC_y

Cuts at:      0     281     378 
   Size:        281      97
 
BstZ17I GTA'TAC

Cuts at:      0     120     378 
   Size:        120     258
 
BtgI C'CryG_G

Cuts at:      0     176     378 
   Size:        176     202
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     204     378 
   Size:        204     174
 
DraIII CAC_nnn'GTG

Cuts at:      0     333     378 
   Size:        333      45
 
EaeI y'GGCC_r

Cuts at:      0     290     320     378 
   Size:        290      30      58

  Fragments arranged by size: 

                 290      58      30
 
EagI C'GGCC_G

Cuts at:      0     290     378 
   Size:        290      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     258     378 
   Size:        157     101     120

  Fragments arranged by size: 

                 157     120     101
 
HaeII r_GCGC'y

Cuts at:      0     261     374     378 
   Size:        261     113       4

  Fragments arranged by size: 

                 261     113       4
 
HincII GTy'rAC

Cuts at:      0     201     378 
   Size:        201     177
 
MscI TGG'CCA

Cuts at:      0     322     378 
   Size:        322      56
 
NcoI C'CATG_G

Cuts at:      0     176     378 
   Size:        176     202
 
SmaI CCC'GGG

Cuts at:      0     312     378 
   Size:        312      66
 
StyI C'CwwG_G

Cuts at:      0     176     347     378 
   Size:        176     171      31

  Fragments arranged by size: 

                 176     171      31
 
TatI w'GTAC_w

Cuts at:      0     125     362     378 
   Size:        125     237      16

  Fragments arranged by size: 

                 237     125      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     319     351     378 
   Size:        319      32      27

  Fragments arranged by size: 

                 319      32      27
 
XmaI C'CCGG_G

Cuts at:      0     310     378 
   Size:        310      68
 
XmnI GAAnn'nnTTC

Cuts at:      0     193     378 
   Size:        193     185
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AvaI     BanI    BanII Bme1580I
     BplI     BpmI    BsaAI    BseRI    BsiEI Bsp1286I    BspHI    BspMI
    BsrBI    BsrFI    BsrGI    BstBI    BstYI  BstZ17I     BtgI    BtgZI
   DraIII     EaeI     EagI  Eco57MI    HaeII   HincII     MscI     NcoI
     SmaI     StyI     TatI     TstI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrDI
   BssHII    BssSI   BstAPI   BstEII    BstXI   Bsu36I     BtsI     ClaI
    CspCI     DraI     DrdI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XhoI     ZraI